本文選題：前列腺增生 + 模型　； 參考：《寧夏醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:1.建立大鼠前列腺增生動物模型,篩選丙酸睪酮誘導(dǎo)去勢大鼠前列腺增生模型的造模最佳時間點。2.探討去勢大鼠前列腺增生模型注射重組鼠源性HMGB1蛋白對大鼠前列腺的影響及其可能機制。方法:1.48只成年雄性SD大鼠隨機分成為6組,每組8只:對照組、去勢對照組、7d組(皮下注射丙酸睪酮7天后取材)、14d組、21d組、28d組;測量大鼠體重、前列腺體積、濕重及前列腺指數(shù);采用HE染色法觀察前列腺組織病理學(xué)改變;免疫組織化學(xué)及Western blot法檢測大鼠前列腺組織中HMGB1、RAGE、TLR4的表達(dá)變化。2.32只成年雄性SD大鼠隨機分成為4組,每組8只:21d模型組、低劑量組(去勢后往大鼠前列腺組織注射100mg重組鼠源性HMGB1蛋白,同時皮下注射丙酸睪酮21天后取材)、中劑量組(200mg)、高劑量組(400mg);測量大鼠體重、前列腺體積、濕重及前列腺指數(shù);采用HE染色法觀察前列腺組織病理學(xué)改變;免疫組織化學(xué)及Western blot法檢測大鼠前列腺組織中HMGB1、RAGE、TLR4的表達(dá)變化。結(jié)果:1.大鼠去勢后前列腺組織萎縮,與對照組及7d組比較,14d組、21d及28d組大鼠前列腺體積、前列腺濕重、前列腺指數(shù)均明顯增加(P0.05)。且21d組與28d組前列腺體積較14d組明顯增大(P0.05),但21d組與28d間各數(shù)值無統(tǒng)計學(xué)差異。14d組大鼠前列腺上皮細(xì)胞和間質(zhì)組織出現(xiàn)增生改變,21d和28d組大鼠前列腺上皮細(xì)胞和間質(zhì)組織明顯增生,且存在炎性細(xì)胞浸潤。與對照組比較,21d組大鼠前列腺組織HMGB1、RAGE、TLR4的表達(dá)升高(P0.05);與對照組比較,28d組大鼠前列腺組織HMGB1、TLR4的表達(dá)升高(P0.05)。2.與21d組比較,HMGB1中、高劑量組指數(shù)明顯增加(P0.05),其余組之間無統(tǒng)計學(xué)差異;與21d組比較,HMGB1大劑量組前列腺組織中HMGB1、RAGE、TLR4的表達(dá)明顯增加(P0.05),其余組之間無統(tǒng)計學(xué)差異。結(jié)論:1.采用去勢后皮下注射丙酸睪酮的方法,21天就可成功構(gòu)建大鼠前列腺增生模型,且在此過程中伴隨HMGB1、RAGE、TLR4的表達(dá)升高。2.采用去勢大鼠前列腺增生模型中注射中、高劑量重組鼠源性HMGB1蛋白的方法,可使大鼠前列腺指數(shù)明顯增加,且在此過程中伴隨HMGB1、RAGE、TLR4的表達(dá)升高。
[Abstract]:Purpose 1. To establish an animal model of prostatic hyperplasia in rats, the optimal time point of prostatic hyperplasia model induced by testosterone propionate in ovariectomized rats was selected. To investigate the effect of recombinant murine HMGB1 protein injection on rat prostate and its possible mechanism in ovariectomized rats with benign prostatic hyperplasia (BPH). Methods one hundred and forty-eight adult male Sprague-Dawley rats were randomly divided into 6 groups: control group (n = 8) and castrated control group (n = 7). Wet weight and prostate index; HE staining method were used to observe the pathological changes of prostate tissue; immunohistochemistry and Western blot method were used to detect the expression of HMGB1 and RAGEGE-TLR4. 2.32 adult male Sprague-Dawley rats were randomly divided into 4 groups. Eight rats in each group were divided into two groups: low dose group (100mg recombinant rat HMGB1 protein was injected into prostate tissue after castration, testosterone propionate was injected subcutaneously for 21 days), middle dose group (200 mg / g), high dose group (400 mg / g), body weight and volume of prostate were measured. The wet weight and prostate index, the pathological changes of prostate tissue were observed by HE staining, and the expression of HMGB1 and RAGEGE-TLR4 in rat prostate tissue was detected by immunohistochemistry and Western blot. The result is 1: 1. Compared with control group and 7 day group, the prostate volume, wet weight and prostatic index of rats in 14 d group and 28 d group were significantly increased compared with control group and 7 d group. The prostate volume of 21 d group and 28 d group was significantly larger than that of 14 d group, but there was no significant difference between 21 d group and 28 d group. There was no significant difference between 21 d group and 28 d group. The interstitial tissue was obviously proliferated. And inflammatory cell infiltration. Compared with the control group, the expression of HMGB1-RAGEGE-TLR4 in the prostate tissue of rats in 21 d group was higher than that in the control group, and the expression of HMGB1 + TLR4 in the prostate tissue of the control group was significantly higher than that in the control group. Compared with the 21D group, the index of HMGB1 increased significantly in high dose group, but there was no statistical difference between the other groups, and the expression of HMGB1 RAGEGE-TLR4 in prostate tissue of high-dose HMGB1 group was significantly higher than that of 21D group, but there was no statistical difference between the other groups. Conclusion 1. The rat model of prostatic hyperplasia was successfully constructed by subcutaneous injection of testosterone propionate 21 days after ovariectomy, and the expression of TLR4 was increased with the increase of HMGB1, RAGEG, TLR4. The high dose recombinant HMGB1 protein in ovariectomized rat model of prostatic hyperplasia could significantly increase the prostatic index, and the expression of HMGB1 / RAGEGE-TLR4 was increased in the process.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R697.3

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本文編號：2019757