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  本文選題：遠(yuǎn)程缺血預(yù)處理 + 缺血再灌注損傷�。� 參考：《浙江大學(xué)》2014年碩士論文

【摘要】：目的：通過建立腎臟缺血再灌注損傷動物模型,研究肢體遠(yuǎn)程缺血預(yù)處理對小鼠腎缺血再灌注損傷的保護(hù)作用,并探討其可能的機(jī)制。 實(shí)驗(yàn)方法：C57BL/6雄性小鼠15只,8～10周齡,體重20～25g,隨機(jī)分為假手術(shù)組(Sham組)、缺血再灌注組(I/R組)、肢體遠(yuǎn)程缺血預(yù)處理組(RIPC組),每組5只。假手術(shù)組(Sham組)打開腹腔,分離左側(cè)腎蒂,但不阻斷腎蒂,曠置觀察35min；缺血再灌注組(I/R)打開腹腔,分離左側(cè)腎蒂,用無損動脈夾夾閉左側(cè)腎蒂,35min后去除動脈夾并切除右側(cè)腎臟,再灌注24h；肢體遠(yuǎn)程缺血預(yù)處理組(RIPC組)：用橡皮經(jīng)結(jié)扎小鼠下肢根部5min,松開5min,反復(fù)4次行肢體遠(yuǎn)程缺血預(yù)處理,在最后一次5min再灌注后立即打開腹腔,分離左側(cè)腎蒂,用無損動脈夾阻斷左側(cè)腎蒂,35min后去除動脈夾并切除右側(cè)腎臟,再灌注24h。于再灌注24h后眼眶取血留取血清標(biāo)本測定血清肌酐及尿素氮,采用Luminex的方法檢測血清腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-6(IL-6)、白細(xì)胞介素-10(IL-10)濃度。處死小鼠留取腎組織伊紅染色觀察腎組織病理學(xué)。 結(jié)果：再灌注24h后,缺血再灌注組與假手術(shù)組相比,血清肌酐濃度、尿素氮濃度和腎組織病理學(xué)評分均顯著升高(p0.05),血清TNF-α、IL-6、濃度均升高(p0.05),血清IL-10濃度差異無統(tǒng)計(jì)學(xué)意義,(p0.05)；與缺血再灌注組比較,遠(yuǎn)程缺血預(yù)處理組血清肌酐、尿素氮濃度及腎組織病理學(xué)評分均降低,血清TNF-α、IL-6濃度降低,血清IL-10濃度升高(p0.05)。 結(jié)論：肢體遠(yuǎn)程缺血預(yù)處理對小鼠腎臟缺血再灌注損傷具有保護(hù)作用。肢體遠(yuǎn)程缺血預(yù)處理可能通過抑制促炎癥細(xì)胞因子TNF-α、IL-6的釋放,增加抗炎癥因子IL-10的合成,調(diào)整機(jī)體促炎癥與抗炎癥的平衡來實(shí)現(xiàn)腎臟的保護(hù)作用。
[Abstract]:Objective: to study the protective effect of limb remote ischemic preconditioning on renal ischemia-reperfusion injury in mice by establishing an animal model of renal ischemia-reperfusion injury, and to explore its possible mechanism. The rats were randomly divided into sham-operated group, ischemia-reperfusion group and limb remote ischemic preconditioning group, with 5 rats in each group. Sham group (sham group) opened the abdominal cavity, separated the left renal pedicle, but did not block the renal pedicle, and observed the left renal pedicle for 35 min. In the ischemia reperfusion group, I / R) opened the abdominal cavity, separated the left renal pedicle, clipped the left renal pedicle for 35 minutes and removed the artery clip and removed the right kidney. Limb remote ischemic preconditioning group (RIPC group): ligated the lower extremity root of mice with rubber for 5 minutes, loosened for 5 minutes, repeated four times of limb remote ischemic preconditioning, immediately after the last 5min reperfusion, opened the abdominal cavity and separated the left renal pedicle. The left renal pedicle was blocked with a non-invasive artery clip for 35 minutes, then the artery clip was removed and the right kidney was resected for 24 h after reperfusion. Serum creatinine and urea nitrogen were measured 24 hours after reperfusion. Serum levels of TNF- 偽, IL-6 and IL-10 were determined by Luminex method. Results: 24 hours after reperfusion, serum creatinine concentration in ischemia reperfusion group was higher than that in sham operation group. The concentrations of urea nitrogen and renal histopathology were significantly increased, serum TNF- 偽 and IL-6 were increased, and the concentrations of IL-10 were not significantly different from those in the ischemic reperfusion group, compared with those in the ischemic reperfusion group, the serum creatinine in the remote preconditioning group was higher than that in the ischemic preconditioning group, and there was no significant difference in the concentration of IL-10 between the two groups. The concentrations of urea nitrogen and renal histopathology were decreased, the concentration of serum TNF- 偽 and IL-6 decreased, and the concentration of serum IL-10 increased (p0.05). Conclusion: limb remote ischemic preconditioning has protective effect on renal ischemia-reperfusion injury in mice. Limb remote ischemic preconditioning may protect the kidney by inhibiting the release of TNF- 偽 IL-6, increasing the synthesis of anti-inflammatory cytokine IL-10 and adjusting the balance between pro-inflammatory and anti-inflammatory.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R692

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相關(guān)期刊論文 前1條

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