發(fā)布時(shí)間：2018-06-08 04:25

  本文選題：早期生長(zhǎng)反應(yīng)蛋白 + 基因轉(zhuǎn)染��； 參考：《中國(guó)病理生理雜志》2015年09期

【摘要】：目的:觀察Egr-1基因轉(zhuǎn)染對(duì)糖尿病小鼠腎組織腫瘤壞死因子α(TNF-α)及細(xì)胞間黏附分子1(ICAM-1)表達(dá)的影響,探討Egr-1在糖尿病腎病發(fā)病機(jī)制中的作用。方法:制備糖尿病小鼠模型,成模后隨機(jī)選取10只作為糖尿病組,剩余40只每周1次經(jīng)尾靜脈分別注射空質(zhì)粒、Egr-1表達(dá)質(zhì)粒和Egr-1 siRNA質(zhì)粒,并設(shè)立正常對(duì)照組。實(shí)驗(yàn)共4周,于第4周末收集各組小鼠腎組織標(biāo)本,分別應(yīng)用western blot和免疫組織化學(xué)染色測(cè)定腎組織中Egr-1、TNF-α和ICAM-1的表達(dá);應(yīng)用電鏡觀察腎小球超微結(jié)構(gòu)的改變。結(jié)果:糖尿病小鼠腎組織Egr-1、TNF-α及ICAM-1表達(dá)增強(qiáng),基底膜普遍增厚,系膜區(qū)基質(zhì)增多,上皮細(xì)胞足突融合;Egr-1基因轉(zhuǎn)染后上述變化趨勢(shì)更加顯著。siRNA質(zhì)粒轉(zhuǎn)染組上述變化較糖尿病組明顯減輕。結(jié)論:Egr-1可上調(diào)TNF-α及ICAM-1表達(dá),促進(jìn)系膜細(xì)胞增殖及系膜外基質(zhì)積聚,可能是加速腎小球硬化的可能機(jī)制之一。
[Abstract]:Aim: to investigate the effect of Egr-1 gene transfection on the expression of TNF- 偽 TNF- 偽 and ICAM-1 in renal tissue of diabetic mice, and to explore the role of Egr-1 in the pathogenesis of diabetic nephropathy. Methods: ten diabetic mice were randomly selected as diabetic group. The remaining 40 mice were injected with empty plasmids Egr-1 and Egr-1 siRNA plasmids via caudal vein once a week, and normal control group was set up. At the end of the 4th week, the expression of Egr-1 TNF- 偽 and ICAM-1 were detected by western blot and immunohistochemical staining, and the ultrastructure of glomeruli was observed by electron microscope. Results: the expression of Egr-1 TNF- 偽 and ICAM-1 was increased, basement membrane thickened and Mesangial matrix increased in diabetic mice. The changes of Egr-1 gene in epithelial cells were more significant after transfection of Egr-1 gene. The changes of Egr-1 gene in the siRNA plasmid transfection group were significantly less than those in the diabetic group. Conclusion the expression of TNF- 偽 and ICAM-1 can be upregulated, and the proliferation of Mesangial cells and the accumulation of Mesangial matrix can be promoted by WEgr-1, which may be one of the possible mechanisms of accelerating glomerulosclerosis.
【作者單位】： 河北大學(xué)附屬醫(yī)院內(nèi)分泌科;河北大學(xué)附屬醫(yī)院腎內(nèi)科;滿城縣醫(yī)院腫瘤內(nèi)分泌科;保定市第一醫(yī)院兒科;河北大學(xué)附屬醫(yī)院科研處;
【基金】：保定市科學(xué)技術(shù)研究與發(fā)展指導(dǎo)計(jì)劃項(xiàng)目(No.13ZF115)
【分類號(hào)】：R587.2;R692.9

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本文編號(hào)：1994448