本文選題：溶瘤腺病毒 + 膀胱腫瘤細胞��； 參考：《蘭州大學》2014年碩士論文

【摘要】：目的：探討溶瘤腺病毒聯(lián)合放療對膀胱腫瘤細胞的影響。 方法：使用CCK-8(cell counting kit-8)法檢測對膀胱腫瘤細胞生存率的影響；流式細胞儀檢測細胞周期和凋亡率；實時熒光定量PCR檢測Bax、Bcl-2mRNA,自噬相關基因BECN、Atg-5mRNA, CAR mRNA表達情況；Western blot檢測E1A、Bax、Bcl-2、細胞色素C蛋白表達情況。 結果：聯(lián)合治療可以明顯降低膀胱腫瘤細胞的生存率,呈劑量依賴性,并且具有協(xié)同作用；流式細胞儀檢測顯示聯(lián)合治療可以誘導膀胱腫瘤細胞周期阻滯并發(fā)生凋亡；實時熒光定量PCR檢測顯示聯(lián)合治療組Bax mRNA表達升高(p0.05),Bcl-2mRNA表達下降(p0.05)；自噬相關基因BECN, Atg-5mRNA與對照組相比表達下降(p0.05)；聯(lián)合組與單純作用組相比CAR mRNA表達水平是增高的(p0.05)；Western blot檢測顯示實驗組E1A蛋白、Bax、細胞質中細胞色素C增高,Bcl-2、線粒體中細胞色素C降低。 結論：聯(lián)合治療組對腫瘤細胞殺傷具有協(xié)同效應有可能是通過線粒體凋亡通路來誘導膀胱腫瘤細胞發(fā)生凋亡,從而發(fā)揮抑制腫瘤增殖效應。
[Abstract]:Objective: to investigate the effect of adenolysin combined with radiotherapy on bladder tumor cells. Methods: CCK-8(cell counting kit-8 method was used to detect the survival rate of bladder tumor cells, flow cytometry was used to detect cell cycle and apoptosis rate. Baxan Bcl-2 mRNAs were detected by real-time fluorescence quantitative PCR, autophagy related gene BECN-5mRNAs, CAR mRNA expression was detected by Western blot, and cytochrome C protein expression was detected by Western blot. Results: combined therapy could significantly reduce the survival rate of bladder tumor cells in a dose-dependent manner and had synergistic effects. Flow cytometry showed that combined therapy could induce cell cycle arrest and apoptosis of bladder tumor cells. Real-time fluorescence quantitative PCR analysis showed that the expression of Bax mRNA increased in the combined treatment group and decreased in the combination group. The expression of autophagy related gene BECNN, Atg-5mRNA was lower than that of the control group, and the expression level of CAR mRNA in the combined group was higher than that in the control group. The expression level of CAR mRNA in the combined group was higher than that in the control group, and the expression level of CAR mRNA in the combined group was higher than that in the control group. The results showed that cytochrome C in cytochrome C increased and cytochrome C in mitochondria decreased in the experimental group. Conclusion: the synergistic effect of combined treatment group on tumor cell killing may be induced by mitochondrial apoptosis pathway to induce apoptosis of bladder tumor cells and thus play a role in inhibiting tumor proliferation.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.14

【參考文獻】

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1 鄒曉峰;張國璽;肖日海;袁源湖;廖云峰;伍耿青;王曉寧;吳玉婷;薛義軍;劉佛林;楊軍;鐘辛;徐剛;江波;龍大治;徐輝;劉敏;;經(jīng)陰道純NOTES腎囊腫去頂術1例報告并文獻復習[J];臨床泌尿外科雜志;2011年03期

2 鄒曉峰;張國璽;肖日海;袁源湖;龍大治;劉佛林;伍耿青;王曉寧;薛義軍;楊軍;吳玉婷;徐輝;廖云峰;鐘辛;江波;徐剛;;經(jīng)陰道純NOTES腎切除術2例報告并文獻復習[J];臨床泌尿外科雜志;2011年09期

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本文編號：1988900