本文選題：RNA干擾 + 前列腺腫瘤��； 參考：《第二軍醫(yī)大學學報》2016年06期

【摘要】：目的采用RNA干擾(RNAi)技術抑制前列腺癌PC3細胞系中Bloom解旋酶基因的表達,探討B(tài)loom解旋酶基因表達下調(diào)后對PC3細胞的抑制作用。方法使用實驗室前期成功構建的兩條針對于Bloom解旋酶基因的RNAi載體shRNA-1和shRNA-2轉染前列腺癌PC3細胞,以未轉染RNAi載體為對照組,分別在轉染24、48、72h后通過MTT法檢測細胞增殖情況,轉染48h后通過Transwell小室法檢驗細胞侵襲、遷移能力,細胞劃痕實驗檢測細胞遷移情況,Hoechst/PI雙染法檢測細胞凋亡情況。結果轉染RNAi載體后,與未轉染對照組相比,各實驗時間點的轉染組細胞增殖率降低(P0.05),Transwell細胞侵襲和遷移實驗中穿過室膜的細胞數(shù)與對照組比均減少(侵襲:119±24、118±30vs 227±38;遷移:122±13、121±47vs 277±32,P0.05),劃痕愈合率與劃痕遷移距離均降低,48h時差異有統(tǒng)計學意義(P0.05),而且細胞凋亡明顯增加。結論以RNAi載體干擾Bloom解旋酶基因的表達可抑制前列腺癌PC3細胞的增殖、遷移和侵襲能力并促進其凋亡,為前列腺癌的靶向基因治療提供了依據(jù)。
[Abstract]:Objective to inhibit the expression of Bloom helicase gene in prostate cancer PC3 cell line by RNA interference RNAi technique and to investigate the inhibitory effect of Bloom helicase gene expression on PC3 cells. Methods two RNAi vectors shRNA-1 and shRNA-2 targeting the Bloom helicase gene were used to transfect the prostate cancer PC3 cells. The untransfected RNAi vector was used as the control group. The proliferation of the cells was detected by MTT assay 72 hours after transfection. After 48 hours of transfection, cell invasion and migration were detected by Transwell chamber assay, cell migration was detected by scratch assay and apoptosis was detected by Hoechst/ Pi double staining. Results after transfection of RNAi vector, compared with the control group, The number of cells passing through the ventricular membrane in the invasion and migration of P0.05 30vs cells in each experimental time point decreased compared with that in the control group (invasion: 119 鹵24118 鹵30vs 227 鹵38; migration: 122 鹵13121 鹵47vs 277 鹵32P 0.05). The rate of scratch healing and the distance between scratch migration and scratch healing were decreased at 48h. The difference was statistically significant (P 0.05), and apoptosis increased significantly. Conclusion interfering the expression of Bloom helicase gene with RNAi vector can inhibit the proliferation, migration, invasion and apoptosis of prostate cancer PC3 cells, and provide the basis for the targeted gene therapy of prostate cancer.
【作者單位】： 貴州大學生命科學學院;貴州大學動物科學學院高原山地動物遺傳育種與繁殖教育部重點實驗室;貴州大學農(nóng)學院;
【基金】：國家自然科學基金(31361406) 貴州省國際合作項目[黔科合外G字(2011)7008號]~~
【分類號】：R737.25

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本文編號：1974209