本文選題：FSH + 特發(fā)性少弱精子癥�。� 參考：《山東中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：初步探討純尿促卵泡素（FSH）對特發(fā)性少、弱精子癥患者DNA碎片的修復(fù)機(jī)理。通過分析比較注射FSH治療前后精子濃度、活力、畸形精子率、DNA碎片率及MDA水平，論證FSH能夠改善男性生育能力，初步揭示FSH對特發(fā)性少、弱精子癥患者DNA碎片的修復(fù)機(jī)理。方法：初步篩選2012年11月—2013年8月在我中心就診，并符合特發(fā)性少、弱精子癥西醫(yī)診斷標(biāo)準(zhǔn)的不育患者，入組病人148例，，規(guī)范化采集其精液標(biāo)本，檢測其精液量、精子濃度、活力、畸形精子率以及MDA水平。隨機(jī)分為三組，分別為生精散組50例、FSH組49例、安慰劑組49例。三組患者均不采取生活干預(yù)。生精散組服用生精散（2.3g po tid），用藥3個(gè)月。FSH組肌肉注射FSH（75IU imqod），用藥20天。安慰劑組服用安慰劑（2.3g po tid），用藥3個(gè)月。通過比較各組每個(gè)月治療前后患者精液的諸項(xiàng)指標(biāo)，特別是DNA碎片率，初步探討FSH的作用機(jī)理。結(jié)果：1、各組臨床總體療效治療前后的對比：FSH組和生精散組總體療效優(yōu)于安慰劑組，有統(tǒng)計(jì)學(xué)意義（P＜0.05）。兩組之間總體療效比較無統(tǒng)計(jì)學(xué)意義（P＞0.05）。2、治療前后精子濃度的對比：生精散組和FSH組治療后的精子濃度都顯著提高，有統(tǒng)計(jì)學(xué)意義（P＜0.05），且較安慰劑組治療比較有統(tǒng)計(jì)學(xué)意義（P＜0.05）。治療后，F(xiàn)SH組的精子濃度顯著高于生精散組，有統(tǒng)計(jì)學(xué)意義（P＜0.05）。3、治療前后精子活力的對比：FSH組和生精散組治療后的前向運(yùn)動精子和非前向運(yùn)動精子有顯著提高，有統(tǒng)計(jì)學(xué)意義（P＜0.05），且較安慰劑組治療比較有統(tǒng)計(jì)學(xué)意義（P＜0.05），但FSH組和生精散組之間比較無統(tǒng)計(jì)學(xué)意義（P＞0.05）。4、治療前后精子畸形率的比較：FSH組治療后的畸形精子率顯著降低，有統(tǒng)計(jì)學(xué)意義（P＜0.05），且較生精散組和安慰劑組治療后比較有統(tǒng)計(jì)學(xué)意義（P＜0.05）。生精散組和安慰劑組治療后比較無統(tǒng)計(jì)學(xué)意義（P＞0.05）。5、治療前后精子DNA碎片率的比較：FSH組和生精散組治療后的精子DNA碎片率有顯著降低，有統(tǒng)計(jì)學(xué)意義（P＜0.05），且較安慰劑組比較有統(tǒng)計(jì)學(xué)意義（P＜0.05），但FSH組和生精散組之間比較無統(tǒng)計(jì)學(xué)意義（P＞0.05）。6、治療前后精漿MDA水平的比較：FSH組和生精散組治療后MDA水平有顯著降低，有統(tǒng)計(jì)學(xué)意義（P＜0.05），且較安慰劑組比較有統(tǒng)計(jì)學(xué)意義（P＜0.05），但FSH組和生精散組之間比較無統(tǒng)計(jì)學(xué)意義（P＞0.05）。結(jié)論：本研究表明精子活力與DNA碎片率具有相關(guān)性。FSH通過降低MDA水平，提高精子活力，降低精子DNA碎片率。能夠明顯改善特發(fā)性少、弱精子癥患者精液質(zhì)量（包括提高精子濃度，提高前向運(yùn)動精子和非前向運(yùn)動精子活力，降低精子畸形率等），進(jìn)而提高其生育能力。MDA水平與精子DNA碎片率呈正相關(guān)。
[Abstract]:Aim: to investigate the repair mechanism of DNA fragments in patients with idiopathic oligozoospermia by pure urinary follicle stimulating hormone (FSH). By analyzing and comparing sperm concentration, motility, abnormal sperm rate and MDA level before and after FSH injection, it was demonstrated that FSH could improve male fertility, and the repair mechanism of DNA fragments in patients with idiopathic and weak spermatospermia was preliminarily revealed by FSH. Methods: a total of 148 infertile patients who met the diagnostic criteria of idiopathic oligozoospermia and western medicine were preliminarily selected from November 2012 to August 2013. The semen samples were collected from 148 patients, and their semen volume and sperm concentration were measured. Motility, abnormal sperm rate and MDA level. They were randomly divided into three groups: 50 cases of FSH group and 49 cases of placebo group. Three groups of patients did not take life intervention. Shengjingsan group was given 2. 3 g of Shengjingsan po tidai, and the group of 3 months. FSH group was intramuscularly injected with FSH(75IU imqodus for 20 days. The placebo group received placebo 2.3 g po tidd for 3 months. The action mechanism of FSH was preliminarily discussed by comparing the indexes of semen, especially the rate of DNA fragments, before and after every month of treatment in each group. Results compared with the control group before and after treatment, the total curative effect of the two groups was better than that of the placebo group (P < 0.05). There was no significant difference in the total therapeutic effect between the two groups (P > 0.05). The sperm concentration in Shengjingsan group and FSH group was significantly increased after treatment (P < 0.05), and was significantly higher than that in placebo group (P < 0.05). After treatment, the sperm concentration in FSH group was significantly higher than that in Shengjingsan group (P < 0.05). The sperm motility of FSH group was significantly higher than that of non-forward motile sperm group after treatment. There was significant difference (P < 0.05) between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjingsan group (P > 0.05). There was significant difference (P < 0.05) between Shengjingsan group and placebo group (P < 0.05). There was no significant difference after treatment between Shengjingsan group and placebo group (P > 0.05). The rate of sperm DNA fragment was significantly decreased in the two groups after treatment. There was significant difference (P < 0.05) between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjingsan group (P > 0.05). The level of MDA in seminal plasma was significantly lower in the control group than that in the control group before and after treatment, and the level of MDA in the control group was significantly lower than that in the control group (P < 0.05). There was significant difference between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjing San group (P > 0.05). Conclusion: this study indicates that sperm motility is correlated with DNA fragment rate. FSH can decrease MDA level, increase sperm motility and decrease DNA fragment rate. Can significantly improve the semen quality of patients with idiopathic oligozoospermia (including increased sperm concentration, forward motile sperm and non-forward motile sperm motility). There was a positive correlation between the sperm deformity rate and the rate of sperm DNA fragments.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R698

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 曹博,鄭俊波,郭筠秋;大鼠睪丸支持細(xì)胞的分離純化與鑒定[J];解剖科學(xué)進(jìn)展;2004年01期

2 王益鑫,張賢生,冷靜,陳斌,韓銀發(fā);156例男性性腺功能低下癥的診治[J];上海第二醫(yī)科大學(xué)學(xué)報(bào);2004年S1期

3 陳暉,陳松,費(fèi)江楓,趙躍華,金偉,曹堅(jiān),費(fèi)仁仁;1050例不育男性精子堿性核蛋白的分析[J];生殖醫(yī)學(xué)雜志;2005年03期

4 鄒俊華,梁紅業(yè),閔凌峰,吳白燕;枸杞子的抗衰老功效及增強(qiáng)DNA修復(fù)能力的作用[J];中國臨床康復(fù);2005年11期

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