本文選題：可溶性CD + 早期糖尿病腎病�。� 參考：《中國醫(yī)科大學(xué)學(xué)報(bào)》2017年11期

【摘要】：目的探討血清可溶性CD146(s CDl46)水平在早期糖尿病腎病(DN)診斷中的作用。方法選用SPF級(jí)SD雄性大鼠32只,隨機(jī)分為2組:空白組(C組)和模型組(DN組),每組16只。造模開始至實(shí)驗(yàn)結(jié)束,每周1次測量大鼠體質(zhì)量、血糖,收集24 h尿液測定尿白蛋白、尿α1徽球蛋白(AIM)、尿轉(zhuǎn)鐵蛋白(TRU)、尿糖定性、尿N-乙酰-β-D-氨基葡萄糖苷酸(NAG)等指標(biāo)。分別于成模第1天、第3周2個(gè)時(shí)間點(diǎn),測定血肌酐(Scr)、血尿素氮(BUN)、s CD146水平,并計(jì)算大鼠腎質(zhì)量/體質(zhì)量比(RW/BW)。腎組織PAS染色觀察腎皮質(zhì),計(jì)算腎小球系膜面積(MMA)。結(jié)果 DN組大鼠尿白蛋白、AIM、TRU、NAG指標(biāo)明顯高于C組(均P0.01)。模型制備成功后第3周Scr、BUN指標(biāo)DN組均高于C組,但差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。模型制備成功后第1天、第3周模型組血清s CD146水平均較C組增高(均P0.05)。RW/BW以及腎組織PAS染色結(jié)果顯示,DN組RW/BW以及MMA均高于C組(P0.01)。結(jié)論與C組比較,DN組中血清s CD146水平較早較快增加,s CD146能夠更敏感地反映腎臟損害程度,對(duì)監(jiān)測DN的發(fā)生與發(fā)展有重要臨床意義。
[Abstract]:Objective to investigate the role of serum soluble CD146(s CDL 46 in the diagnosis of early diabetic nephropathy. Methods Thirty-two SPF male SD rats were randomly divided into two groups: blank group (C group) and model group (16 rats in each group). From the beginning to the end of the experiment, the body mass and blood sugar of rats were measured once a week. Urine albumin, urine 偽 1 globulin (AIMA), urinary transferrin (TRU), urine glucose and urine N-acetyl- 尾 -Daminoglycoside acid (NAG) were collected for 24 hours to determine urinary albumin, urinary 偽 1 globulin, urinary transferrin, urine sugar and urine N-acetyl- 尾 -Daminoglycoside acid (NAG). The serum creatinine (SCR) and blood urea nitrogen (bun) CD146 were measured at the first day and the third week of the model, and the RW / BW ratio was calculated. Renal cortex was observed by PAS staining and glomerular Mesangial area was calculated. Results urinary albumin in DN group was significantly higher than that in C group (P 0.01). The Scrn bun index in DN group was higher than that in C group at the 3rd week after the model was made, but the difference was not statistically significant (P 0.05). The serum s CD146 levels in the model group were higher than those in the C group at the 3rd week after the model was successfully prepared. The results of P0.05).RW/BW and PAS staining showed that the RW/BW and MMA in the DN group were higher than those in the C group (P 0.01). Conclusion compared with group C, the level of serum s CD146 in DN group can be more sensitive to reflect the degree of renal damage than that in group C, and it is of great clinical significance to monitor the occurrence and development of DN.
【作者單位】： 中國醫(yī)科大學(xué)附屬盛京醫(yī)院實(shí)驗(yàn)研究中心動(dòng)物實(shí)驗(yàn)室;中國醫(yī)科大學(xué)附屬盛京醫(yī)院內(nèi)分泌科;
【基金】：沈陽市科學(xué)技術(shù)計(jì)劃(F13-316-1-32)
【分類號(hào)】：R587.2;R692.9

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