本文選題：大鼠 + 輸精管結(jié)扎�。� 參考：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:定量研究大鼠遠(yuǎn)端輸精管結(jié)扎對睪丸間質(zhì)內(nèi)各類細(xì)胞總數(shù)的影響。方法:12只成年雄性Sprague-Dawley大鼠,經(jīng)腹股溝管行單側(cè)遠(yuǎn)端輸精管結(jié)扎,術(shù)后37天取雙側(cè)睪丸,制作甲基丙烯酸樹脂包埋切片,用過碘酸-Schiff試劑和蘇木精染色后進(jìn)行組織學(xué)觀察,并運(yùn)用體視學(xué)方法——光學(xué)體視框——定量研究睪丸間質(zhì)內(nèi)所有種類細(xì)胞(紅細(xì)胞除外)的總數(shù)。結(jié)果:與對照側(cè)相比,5只大鼠結(jié)扎側(cè)睪丸的精子發(fā)生有明顯損害。不論精子發(fā)生是否損害,結(jié)扎側(cè)睪丸間質(zhì)內(nèi)Leydig細(xì)胞、內(nèi)皮細(xì)胞或肌樣細(xì)胞的總數(shù),都無顯著性變化。結(jié)論:大鼠輸精管結(jié)扎短期內(nèi)對睪丸間質(zhì)的細(xì)胞數(shù)沒有影響,對精子發(fā)生的影響與Leydig細(xì)胞數(shù)無關(guān)。
[Abstract]:Aim: to study quantitatively the effect of distal vasectomy on the total number of various cells in rat testis stroma. Methods Twenty twelve adult male Sprague-Dawley rats were treated with unilateral distal vasectomy via inguinal canal. After 37 days of operation, the bilateral testis were taken and the sections were made into methacrylic resin embedded sections. The specimens were stained with iodate -Schiff reagent and hematoxylin for histological observation. The total number of all kinds of cells (except red blood cells) in the stroma of testis was quantitatively studied by stereological method-optical stereoscopic frame. Results: the spermatogenesis of testis ligated in 5 rats was significantly damaged compared with the control side. There was no significant change in the total number of Leydig cells, endothelial cells or myoid cells in the stroma of the ligated testis, regardless of whether the spermatogenesis was damaged or not. Conclusion: the effect of vasectomy on the number of interstitial cells in testis is not related to the number of Leydig cells.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R699.8

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