發(fā)布時間：2018-05-06 08:48

  本文選題：壓力性尿失禁 + NO��； 參考：《山西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：1．建立壓力性尿失禁大鼠模型，觀察SUI模型大鼠和對照大鼠的泌尿生殖道組織和恥尾肌的組織形態(tài)學(xué)差異。2.檢測SUI大鼠和對照組盆底組織中神經(jīng)遞質(zhì)NO、NPY的表達(dá)情況，探究其與SUI發(fā)生的關(guān)系。 方法：1.40只SD大鼠，，隨機(jī)分為對照組和實(shí)驗(yàn)組。對照組常規(guī)飼養(yǎng)，不作任何處理。實(shí)驗(yàn)組采用模擬難產(chǎn)，常規(guī)飼養(yǎng)一周后切除雙側(cè)卵巢。四周后所有大鼠行尿流動力學(xué)檢測最大膀胱容量和漏尿點(diǎn)壓力，并行噴嚏實(shí)驗(yàn)。取建模成功的15只大鼠作為實(shí)驗(yàn)組。取同樣條件喂養(yǎng)下的正常大鼠20只作為對照組。2.處死所有大鼠，取尿道、陰道前壁及恥尾肌，行HE染色，觀察上述標(biāo)本的組織形態(tài)學(xué)差異。3.行免疫組化染色方法檢測上述標(biāo)本中N0與NPY的表達(dá)，積分光密度值比較采用獨(dú)立樣本t檢驗(yàn)。 結(jié)果：1.對照組和實(shí)驗(yàn)組大鼠最大膀胱容量分別為（1.95±0.13）ml和（1.22±0.15）ml。漏尿點(diǎn)壓力分別為（27.18±4.90）mmHg和（13.63±4.22）mmHg。實(shí)驗(yàn)組最大膀胱容量和漏尿點(diǎn)壓力均小于對照組，差異有統(tǒng)計(jì)學(xué)意義(P0.01)。噴嚏實(shí)驗(yàn)中，SUI組全為陽性，對照組均為陰性。2. HE染色：對照組可見尿道、陰道前壁、恥尾肌染色均勻，結(jié)構(gòu)完整并緊密相連，肌纖維排列整齊；實(shí)驗(yàn)組可見染色不均勻,組織萎縮變薄,肌纖維走形紊亂、斷裂。3.免疫組化染色：對照組大鼠尿道、陰道前壁、恥尾肌一氧化氮(NO)的積分光密度值分別為：（18.49±2.54）；（17.95±1.37）；（14.78±2.62）。實(shí)驗(yàn)組一氧化氮(NO)的積分光密度值分別為：（8.17±0.88）；（8.04±0.73）；（7.19±1.08）。對照組神經(jīng)肽Y的積分光密度值分別為：（14.04±1.68）；（15.18±2.47）；（13.19±1.72）。實(shí)驗(yàn)組神經(jīng)肽Y的積分光密度值分別為：（6.83±0.58）；（7.27±0.91）；（7.37±0.94）。與對照組相比，實(shí)驗(yàn)組大鼠盆底組織中NO與NPY表達(dá)的積分光密度值降低，差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 結(jié)論：l. SUI大鼠盆底肌肉萎縮變薄、肌纖維斷裂使盆底支持力量減弱可能與SUI發(fā)生有關(guān)。2. NO和NPY在壓力性尿失禁大鼠的尿道、陰道前壁和恥尾肌組織中表達(dá)減少，提示其可能參與了SUI的發(fā)生和發(fā)展。
[Abstract]:Purpose 1. A rat model of stress urinary incontinence was established to observe the histomorphologic differences of genitourinary tissues and pubic caudal muscles between SUI model rats and control rats. The expression of neurotransmitter No-NPY in pelvic floor tissues of SUI rats and control group was detected to explore the relationship between NON-NPY and the pathogenesis of SUI. Methods 1.40 Sprague-Dawley rats were randomly divided into control group and experimental group. The control group was fed regularly without any treatment. The experimental group was fed with analogue dystocia for one week and both ovaries were resected. Four weeks later, the maximal bladder volume and leak point pressure were measured by urodynamics in all rats, and sneezing test was performed. Fifteen rats were selected as experimental group. 20 normal rats fed under the same conditions were used as control group. 2. 2. All the rats were killed, the urethra, vagina anterior wall and pubic tail muscle were taken, and HE staining was used to observe the histomorphologic difference of the above specimens. 3. The expression of N0 and NPY in the above specimens was detected by immunohistochemical staining. The integral optical density was compared by independent sample t test. The result is 1: 1. The maximal bladder volume of control group and experimental group were 1.95 鹵0.13)ml and 1.22 鹵0.15 ml, respectively. The pressure of leak point was 27.18 鹵4.90)mmHg and 13.63 鹵4.22mm Hg, respectively. The maximum bladder volume and leak point pressure in the experimental group were lower than those in the control group, and the difference was statistically significant (P 0.01). In the sneezing test, the SUI group was all positive, and the control group was negative. 2. HE staining: in the control group, the urethra, the anterior wall of vagina, the pubic and caudal muscles were stained evenly, the structure was complete and closely connected, and the muscle fibers were arranged neatly. In the experimental group, the staining was uneven, the tissue atrophy became thinner, the muscle fiber was disorganized, and the muscle fiber was broken. 3. Immunohistochemical staining: the integral optical density of nitric oxide (no) in urethra, vagina anterior wall and pubic caudal muscle of rats in control group was 18.49 鹵2.54, 17.95 鹵1.37 and 14.78 鹵2.62, respectively. The integral optical density of nitric oxide (no) in the experimental group was 8.17 鹵0.88, 8.04 鹵0.73, 7.19 鹵1.08, respectively. The integral optical density of neuropeptide Y in the control group was 14.04 鹵1.68, 15.18 鹵2.47, 13.19 鹵1.72, respectively. The integral optical density of neuropeptide Y in the experimental group was 6.83 鹵0.58, 7.27 鹵0.91 and 7.37 鹵0.94, respectively. Compared with the control group, the integral optical density of no and NPY expression in the pelvic floor of the experimental group was significantly lower than that in the control group (P 0.01). Conclusion: 1. In SUI rats, the pelvic floor muscle atrophy became thinner and the pelvic floor support strength decreased due to the breakage of muscle fiber, which may be related to the occurrence of SUI. The decreased expression of no and NPY in urethra, anterior vaginal wall and pubic muscle of rats with stress urinary incontinence suggests that no and NPY may be involved in the occurrence and development of SUI.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R694.54

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