本文選題：前列腺炎 + IL-17　； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]探討細(xì)胞因子IL-17和趨化因子CCL2在實(shí)驗(yàn)性自身免疫性前列腺炎大鼠模型中表達(dá)及其病理作用。[方法]將2個(gè)月齡的SD大鼠44只隨機(jī)分為正常對(duì)照組(Control組,n=10)、模型組(EAP組,n=10);剩余24只建模制成EAP大鼠后隨機(jī)分為他克莫司組(Tacrolimus組,n=8)、塞來(lái)昔布組(Celecoxib組,n=8)和鹽水對(duì)照組(NS組,n=8)。分別將EAP組和Control組進(jìn)行Von Frey filament行為測(cè)試;將Tacrolimus組、Celecoxib組和NS組于干預(yù)治療后進(jìn)行痛覺(jué)測(cè)試。取各組SD大鼠前列腺組織進(jìn)行HE染色和免疫組化、RT-PCR檢測(cè)、Western blots測(cè)定觀察IL-17和CCL2的表達(dá)。[結(jié)果](1)痛覺(jué)反應(yīng)測(cè)試中:EAP組異常痛覺(jué)誘發(fā)明顯多于Control組;Celecoxib組異常下降頻率較快,而Tacrolimus組則是緩慢下降,NS組則僅為小幅下降(P0.05)。(2)HE染色:EAP組大鼠前列腺呈不規(guī)則改變,腺體間質(zhì)內(nèi)淋巴細(xì)胞和中性粒細(xì)胞浸潤(rùn),腺體周?chē)溲?Control組大鼠前列腺未見(jiàn)明顯炎癥細(xì)胞浸潤(rùn)及充血。免疫組化提示:IL-17和CCL2在EAP組中呈陽(yáng)性表達(dá)。(3)RT-PCR結(jié)果、Western blots方法檢測(cè)發(fā)現(xiàn),與Control組相比,建模后50d,EAP大鼠前列腺I(mǎi)L-17和CCL2的表達(dá)明顯上調(diào)(P0.05)。Celecoxib組、Tacrolimus組在干預(yù)后30 d,與NS組相比,大鼠前列腺的IL-17和CCL2表達(dá)均明顯下調(diào)(P0.05)。[結(jié)論]IL-17和CCL2在實(shí)驗(yàn)性自身免疫性前列腺炎發(fā)病中有重要作用,并且可能對(duì)盆腔疼痛有介導(dǎo)作用;他克莫司和塞來(lái)昔布減少EAP大鼠模型前列腺組織中的炎癥細(xì)胞且減輕骨盆疼痛,其作用機(jī)制可能與抑制IL-17和CCL2的產(chǎn)生有關(guān)。
[Abstract]:[objective] to investigate the expression of cytokine IL-17 and chemokine CCL2 in experimental autoimmune prostatitis in rats. [methods] Forty-four SD rats aged 2 months were randomly divided into control group (n = 10) and model group (n = 10), and the remaining 24 rats were randomly divided into two groups: tacrolimus group (Tacrolimus group), celecoxib group (n = 8) and saline control group (NS group, n = 8). The behavior of Von Frey filament was tested in EAP group and Control group, and pain was tested in Tacrolimus group and NS group after intervention. The prostatic tissues of SD rats in each group were stained with HE and detected by RT-PCR. The expression of IL-17 and CCL2 were detected by Western blots. [results] in the pain response test, abnormal nociceptive response was induced more frequently in the Control group than in the Celecoxib group, while in the Tacrolimus group, the abnormal decrease rate was only slightly decreased P0.05N. T2HE staining showed irregular changes in the prostate gland of the rats in the Tacrolimus group. Interstitial lymphocytes and neutrophils were infiltrated in glandular mesenchymal cells, but no inflammatory cell infiltration and congestion were found in the prostate of rats in the control group. Immunohistochemistry showed that the positive expression of CCL2 and IL-17 in EAP group was detected by RT-PCR. Western blots method showed that the expression of IL-17 and CCL2 in the prostate of 50 days after modeling was significantly up-regulated in the control group compared with that in the NS group, compared with the NS group, and the expression of IL-17 and CCL2 in the prostate of 50 d after modeling was significantly up-regulated in the control group, compared with that in the NS group, and the expression of IL-17 and CCL2 in the prostatic gland of the control group was significantly higher than that in the control group (P < 0.05). The expression of IL-17 and CCL2 in rat prostate was significantly down-regulated (P 0.05). [conclusion] IL-17 and CCL2 may play an important role in the pathogenesis of experimental autoimmune prostatitis and may mediate pelvic pain. Tacrolimus and celecoxib reduce inflammatory cells in prostate tissue and relieve pelvic pain in EAP rats. The mechanism of tacrolimus and celecoxib may be related to the inhibition of IL-17 and CCL2 production.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R697.33

【參考文獻(xiàn)】

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本文編號(hào)：1843604