本文選題：腎缺血再灌注損傷 + 磁共振成像�。� 參考：《南方醫(yī)科大學》2017年碩士論文

【摘要】：第一部分磁共振Tlrho成像監(jiān)測大鼠腎臟缺血再灌注損傷進程的初步研究研究目的研究3.0T磁共振Tlrho技術對大鼠腎IRI的發(fā)生、發(fā)展的監(jiān)測價值,判斷診斷大鼠不同腎損傷效能,并初步闡述該模型Tlrho值變化的影響因素。材料與方法制作同時夾閉雙側腎動脈45min制成大鼠腎IRI模型,將對照組及各模型組行磁共振Tlrho檢查,掃描結束采取靜脈血檢測腎功能、處死取腎組織行病理檢查,各組Tlrho值與病理結果統(tǒng)計學分析。結果各組血肌酐及尿素氮與對照組比較,僅1d組明顯升高(P0.05),其余組稍升高,但無統(tǒng)計學差異且與腎損傷評分不存在相關性(P0.05)。對照組及模型組1h、1d、3d、7d、14d、28d 組的 Tlrho 值均數(shù)分別為:149.605±14.77ms、172.560±17.91ms、201.321±21.60ms、271.321±36.59ms、219.703±28.01ms、185.111±17.58ms、170.520±11.61ms,對照組與各模型組的Tlrho值的差異均具有統(tǒng)計學意義(P0.05)。模型組相鄰兩組比較,除了 14d組與28d組之外,其余組間的Tlrho值比較具有統(tǒng)計學差異。Tlrho值與腎損傷評分呈高度正相關,r=0.893,P0.01。利用ROC預測腎損傷由低-高評分組的AUC分別為0.934、0.957、0.946、0.980。結論磁共振Tlrho技術在監(jiān)測大鼠腎IRI隨時間的發(fā)展及判斷腎損傷程度的應用中具有較高的價值。第二部分磁共振T1-mapping技術在大鼠腎IRI模型的初步應用以及與Tlrho成像的對比研究研究目的運用3.0T磁共振T1-mapping技術探討皮髓質T1值在大鼠腎IRI的初步應用。對比研究T1-mapping與Tlrho技術在腎IRI模型的評價效能。材料與方法制作大鼠腎IRI模型后,將對照組及各模型組行磁共振T1-mapping檢查,掃描結束后采取靜脈血檢測腎功能,處死后腎組織行病理檢查,各組皮髓質T1值與病理結果進行統(tǒng)計學分析,各組T1值與Tlrho值對比研究。結果對照組及模型組 lh、1d、3d、7d、14d、28d 髓質 T1 值均數(shù) 1402.172±71.14ms、1398.475±95.54ms、1574.935±87.55ms、1732.881±110.06ms、1710.61±86.21ms、1550.904±127.49ms、1462.976±60.90ms;皮質 T1 值均值分別為1040.278±43.68ms、1094.6±123.00ms、1242.221±89.97ms、1417.038±115.81ms、1376.789±73.40ms、1148.500±90.19ms、1059.579±54.l0ms。對照組與各模型組的髓質、皮質T1值進行比較,均除了與lh及28d組無統(tǒng)計學差異外(P0.05),與其他組的差異均具有統(tǒng)計學意義(P0.05)。模型組相鄰兩組比較,皮髓質相鄰兩個時間點組間比較,均除了 3d組與7d組之間比較無統(tǒng)計學差異外,其余各組間的差異均有統(tǒng)計學意義。髓皮質T1值與腎損傷評分呈高度正相關,髓質相關系數(shù)r=0.769,P0.01,皮質r=0.787,P0.01。利用ROC預測腎損傷由低到高評分組,Tlrho值在各組的AUC均大于對應皮髓質T1值,Tlrho值、髓質 T1 值、皮質 T1 值得 AUC 各組分別為 0.934、0.957、0.946、0.980;0.792、0.834、0.925、0.920;0.836、0.887、0.919、0.915。結論 1、磁共振 T1-mapping成像在大鼠腎IRI模型中具有較高的應用價值。2、Tlrho對大鼠腎IRI模型的損傷程度診斷效能較T1-mapping好,兩種技術聯(lián)合運用,可以提高對腎IRI發(fā)生、發(fā)展的更全面的監(jiān)測能力。
[Abstract]:The first part of magnetic resonance imaging for monitoring Tlrho renal ischemia reperfusion injury in rats to study the process of 3.0T magnetic resonance Tlrho technology on the kidney of IRI rats, the monitoring value of development, determine whether rat renal injury and discuss factors affecting the effectiveness of the model. The changes of Tlrho value of production materials and methods at the same time occlusion of bilateral renal artery 45min into rat renal IRI model, the control group and the model group underwent magnetic resonance Tlrho examination, the end of the scan take venous blood detection of renal function and renal tissue were sacrificed for histological examination, statistical analysis and pathological results were Tlrho value. Results the serum creatinine and urea nitrogen compared with the control group only, group 1D was significantly higher (P0.05), the other group was slightly increased, but the difference was not statistically significant and the score of renal injury and there is no correlation (P0.05). The control group and the model group, 1H, 1D, 3D, 7d, 14d, 28d group Tlrho The mean was 149.605 + 14.77ms, 172.560 + 17.91ms, 201.321 + 21.60ms, 271.321 + 36.59ms, 219.703 + 28.01ms, 185.111 + 17.58ms, 170.520 + 11.61ms, the control group and the model group differences in Tlrho values were statistically significant (P0.05). The model group adjacent to the two groups, except group 14d with the 28d group, the other groups, the Tlrho value was statistically difference in.Tlrho score was positively correlated with renal damage, r=0.893 damage, grouped by the low high rated AUC respectively with high application value 0.934,0.957,0.946,0.980. conclusion MRI Tlrho technique in renal IRI rats were monitored to determine the time with the development and severity of kidney damage the prediction of ROC by P0.01.. The second part renal magnetic resonance T1-mapping technology in the application of rat renal IRI model and Tlrho imaging to study the use of 3.0T magnetic resonance T1-mapping technology study Corticomedular T1 value preliminary application in rat renal IRI. A comparative study on T1-mapping and Tlrho technology in the evaluation of renal IRI model. The efficiency of rat renal IRI model making materials and methods, the control group and the model group underwent magnetic resonance T1-mapping examination, renal function tests take venous blood after scanning, after the execution of the kidney histologic examination, each medullary T1 values were analyzed statistically with pathological results, the T1 values of each group compared with the Tlrho value. The results of the control group and the model group LH, 1D, 3D, 7d, 14d, 28d, medulla T1 values are 1402.172 + 71.14ms, 1398.475 + 95.54ms, 1574.935 + 87.55ms, 1732.881 + 110.06ms. 1710.61 + 86.21ms, 1550.904 + 127.49ms, 1462.976 + 60.90ms; the mean cortical T1 values were 1040.278 + 43.68ms, 1094.6 + 123.00ms, 1242.221 + 89.97ms, 1417.038 + 115.81ms, 1376.789 + 73.40ms, 1148.500 + 90.19ms, 1059.579 + 54.l0ms. and the control group The model group of the medulla, cortex T1 value, are in addition to the LH and 28d group have no significant difference (P0.05), and the other group differences were statistically significant (P0.05). The model group adjacent to the two groups, corticomedular two adjacent time points were compared between the two groups, except between 3D and 7d group were no significant difference among other groups, the differences were statistically significant. Medullary cortical T1 values score was positively correlated with the renal medulla injury, correlation coefficient r=0.769, P0.01, r=0.787 P0.01. by ROC cortex, prediction of renal injury from low to high score, the Tlrho value corresponding to corticomedular T1 was greater than AUC in each group value, Tlrho value, T1 value is T1 medulla, cortex of AUC groups were 0.934,0.957,0.946,0.980; 0.792,0.834,0.925,0.920; 0.836,0.887,0.919,0.915. 1 conclusion, the application value of.2 T1-mapping magnetic resonance imaging with high IRI in kidney in a rat model of Tlrh. O is more effective than T1-mapping in diagnosing the degree of injury of rat kidney IRI model. Combined application of the two technologies can improve the monitoring ability of the occurrence and development of kidney IRI.

【學位授予單位】：南方醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R445.2;R692

【參考文獻】

相關期刊論文 前1條

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本文編號：1745297