本文選題：腎缺血再灌注損傷 + 磁共振成像��； 參考：《南方醫(yī)科大學(xué)》2017年碩士論文

【摘要】：第一部分磁共振Tlrho成像監(jiān)測(cè)大鼠腎臟缺血再灌注損傷進(jìn)程的初步研究研究目的研究3.0T磁共振Tlrho技術(shù)對(duì)大鼠腎IRI的發(fā)生、發(fā)展的監(jiān)測(cè)價(jià)值,判斷診斷大鼠不同腎損傷效能,并初步闡述該模型Tlrho值變化的影響因素。材料與方法制作同時(shí)夾閉雙側(cè)腎動(dòng)脈45min制成大鼠腎IRI模型,將對(duì)照組及各模型組行磁共振Tlrho檢查,掃描結(jié)束采取靜脈血檢測(cè)腎功能、處死取腎組織行病理檢查,各組Tlrho值與病理結(jié)果統(tǒng)計(jì)學(xué)分析。結(jié)果各組血肌酐及尿素氮與對(duì)照組比較,僅1d組明顯升高(P0.05),其余組稍升高,但無(wú)統(tǒng)計(jì)學(xué)差異且與腎損傷評(píng)分不存在相關(guān)性(P0.05)。對(duì)照組及模型組1h、1d、3d、7d、14d、28d 組的 Tlrho 值均數(shù)分別為:149.605±14.77ms、172.560±17.91ms、201.321±21.60ms、271.321±36.59ms、219.703±28.01ms、185.111±17.58ms、170.520±11.61ms,對(duì)照組與各模型組的Tlrho值的差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。模型組相鄰兩組比較,除了 14d組與28d組之外,其余組間的Tlrho值比較具有統(tǒng)計(jì)學(xué)差異。Tlrho值與腎損傷評(píng)分呈高度正相關(guān),r=0.893,P0.01。利用ROC預(yù)測(cè)腎損傷由低-高評(píng)分組的AUC分別為0.934、0.957、0.946、0.980。結(jié)論磁共振Tlrho技術(shù)在監(jiān)測(cè)大鼠腎IRI隨時(shí)間的發(fā)展及判斷腎損傷程度的應(yīng)用中具有較高的價(jià)值。第二部分磁共振T1-mapping技術(shù)在大鼠腎IRI模型的初步應(yīng)用以及與Tlrho成像的對(duì)比研究研究目的運(yùn)用3.0T磁共振T1-mapping技術(shù)探討皮髓質(zhì)T1值在大鼠腎IRI的初步應(yīng)用。對(duì)比研究T1-mapping與Tlrho技術(shù)在腎IRI模型的評(píng)價(jià)效能。材料與方法制作大鼠腎IRI模型后,將對(duì)照組及各模型組行磁共振T1-mapping檢查,掃描結(jié)束后采取靜脈血檢測(cè)腎功能,處死后腎組織行病理檢查,各組皮髓質(zhì)T1值與病理結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析,各組T1值與Tlrho值對(duì)比研究。結(jié)果對(duì)照組及模型組 lh、1d、3d、7d、14d、28d 髓質(zhì) T1 值均數(shù) 1402.172±71.14ms、1398.475±95.54ms、1574.935±87.55ms、1732.881±110.06ms、1710.61±86.21ms、1550.904±127.49ms、1462.976±60.90ms;皮質(zhì) T1 值均值分別為1040.278±43.68ms、1094.6±123.00ms、1242.221±89.97ms、1417.038±115.81ms、1376.789±73.40ms、1148.500±90.19ms、1059.579±54.l0ms。對(duì)照組與各模型組的髓質(zhì)、皮質(zhì)T1值進(jìn)行比較,均除了與lh及28d組無(wú)統(tǒng)計(jì)學(xué)差異外(P0.05),與其他組的差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。模型組相鄰兩組比較,皮髓質(zhì)相鄰兩個(gè)時(shí)間點(diǎn)組間比較,均除了 3d組與7d組之間比較無(wú)統(tǒng)計(jì)學(xué)差異外,其余各組間的差異均有統(tǒng)計(jì)學(xué)意義。髓皮質(zhì)T1值與腎損傷評(píng)分呈高度正相關(guān),髓質(zhì)相關(guān)系數(shù)r=0.769,P0.01,皮質(zhì)r=0.787,P0.01。利用ROC預(yù)測(cè)腎損傷由低到高評(píng)分組,Tlrho值在各組的AUC均大于對(duì)應(yīng)皮髓質(zhì)T1值,Tlrho值、髓質(zhì) T1 值、皮質(zhì) T1 值得 AUC 各組分別為 0.934、0.957、0.946、0.980;0.792、0.834、0.925、0.920;0.836、0.887、0.919、0.915。結(jié)論 1、磁共振 T1-mapping成像在大鼠腎IRI模型中具有較高的應(yīng)用價(jià)值。2、Tlrho對(duì)大鼠腎IRI模型的損傷程度診斷效能較T1-mapping好,兩種技術(shù)聯(lián)合運(yùn)用,可以提高對(duì)腎IRI發(fā)生、發(fā)展的更全面的監(jiān)測(cè)能力。
[Abstract]:The first part of magnetic resonance imaging for monitoring Tlrho renal ischemia reperfusion injury in rats to study the process of 3.0T magnetic resonance Tlrho technology on the kidney of IRI rats, the monitoring value of development, determine whether rat renal injury and discuss factors affecting the effectiveness of the model. The changes of Tlrho value of production materials and methods at the same time occlusion of bilateral renal artery 45min into rat renal IRI model, the control group and the model group underwent magnetic resonance Tlrho examination, the end of the scan take venous blood detection of renal function and renal tissue were sacrificed for histological examination, statistical analysis and pathological results were Tlrho value. Results the serum creatinine and urea nitrogen compared with the control group only, group 1D was significantly higher (P0.05), the other group was slightly increased, but the difference was not statistically significant and the score of renal injury and there is no correlation (P0.05). The control group and the model group, 1H, 1D, 3D, 7d, 14d, 28d group Tlrho The mean was 149.605 + 14.77ms, 172.560 + 17.91ms, 201.321 + 21.60ms, 271.321 + 36.59ms, 219.703 + 28.01ms, 185.111 + 17.58ms, 170.520 + 11.61ms, the control group and the model group differences in Tlrho values were statistically significant (P0.05). The model group adjacent to the two groups, except group 14d with the 28d group, the other groups, the Tlrho value was statistically difference in.Tlrho score was positively correlated with renal damage, r=0.893 damage, grouped by the low high rated AUC respectively with high application value 0.934,0.957,0.946,0.980. conclusion MRI Tlrho technique in renal IRI rats were monitored to determine the time with the development and severity of kidney damage the prediction of ROC by P0.01.. The second part renal magnetic resonance T1-mapping technology in the application of rat renal IRI model and Tlrho imaging to study the use of 3.0T magnetic resonance T1-mapping technology study Corticomedular T1 value preliminary application in rat renal IRI. A comparative study on T1-mapping and Tlrho technology in the evaluation of renal IRI model. The efficiency of rat renal IRI model making materials and methods, the control group and the model group underwent magnetic resonance T1-mapping examination, renal function tests take venous blood after scanning, after the execution of the kidney histologic examination, each medullary T1 values were analyzed statistically with pathological results, the T1 values of each group compared with the Tlrho value. The results of the control group and the model group LH, 1D, 3D, 7d, 14d, 28d, medulla T1 values are 1402.172 + 71.14ms, 1398.475 + 95.54ms, 1574.935 + 87.55ms, 1732.881 + 110.06ms. 1710.61 + 86.21ms, 1550.904 + 127.49ms, 1462.976 + 60.90ms; the mean cortical T1 values were 1040.278 + 43.68ms, 1094.6 + 123.00ms, 1242.221 + 89.97ms, 1417.038 + 115.81ms, 1376.789 + 73.40ms, 1148.500 + 90.19ms, 1059.579 + 54.l0ms. and the control group The model group of the medulla, cortex T1 value, are in addition to the LH and 28d group have no significant difference (P0.05), and the other group differences were statistically significant (P0.05). The model group adjacent to the two groups, corticomedular two adjacent time points were compared between the two groups, except between 3D and 7d group were no significant difference among other groups, the differences were statistically significant. Medullary cortical T1 values score was positively correlated with the renal medulla injury, correlation coefficient r=0.769, P0.01, r=0.787 P0.01. by ROC cortex, prediction of renal injury from low to high score, the Tlrho value corresponding to corticomedular T1 was greater than AUC in each group value, Tlrho value, T1 value is T1 medulla, cortex of AUC groups were 0.934,0.957,0.946,0.980; 0.792,0.834,0.925,0.920; 0.836,0.887,0.919,0.915. 1 conclusion, the application value of.2 T1-mapping magnetic resonance imaging with high IRI in kidney in a rat model of Tlrh. O is more effective than T1-mapping in diagnosing the degree of injury of rat kidney IRI model. Combined application of the two technologies can improve the monitoring ability of the occurrence and development of kidney IRI.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R445.2;R692

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Liver biopsy:complications and risk factors[J];World Journal of Gastroenterology;1999年04期

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本文編號(hào)：1745297