本文選題：膀胱癌　切入點(diǎn)：脫落　出處：《北京化工大學(xué)》2014年碩士論文

【摘要】：膀胱癌是人泌尿系統(tǒng)中一種常見的癌癥,非侵入性早期檢測膀胱癌在臨床診斷上具有重要的意義,并且得到了廣泛的研究。本文通過研究尿液中脫落細(xì)胞捕獲和研究腫瘤相關(guān)基因檢測兩方面內(nèi)容,提高了細(xì)胞的捕獲效率,增強(qiáng)了檢測的靈敏度,希望應(yīng)用于膀胱癌的早期臨床診斷或者預(yù)后中。研究的主要內(nèi)容和結(jié)論如下： 第一章綜述了體液中脫落腫瘤細(xì)胞的分離捕獲方法,詳細(xì)闡述了膀胱癌早期檢測的方法及最新進(jìn)展。在此基礎(chǔ)上提出了論文的研究思路和方案。 第二章膀胱癌發(fā)生發(fā)展過程中腫瘤細(xì)胞會脫落到尿液中,為非侵入性檢測膀胱癌提供了可能。本文通過采用聚碳酸酯微孔膜和鎳微孔篩快速高效的捕獲尿液中的膀胱癌細(xì)胞,并且利用二氯甲烷溶膜的特性,簡單快捷的回收捕獲在聚碳酸酯微孔濾膜上的細(xì)胞。微孔過濾法捕獲細(xì)胞效率85%,分離富集時(shí)間20min,明顯優(yōu)于臨床上傳統(tǒng)的離心分離富集方法。本實(shí)驗(yàn)較為系統(tǒng)的考察了濾膜類型、固定劑以及流速對腫瘤細(xì)胞捕獲效率的影響,該方法速度快,成本低,并且環(huán)境友好,為今后應(yīng)用于臨床診斷研究提供了可能性及理論依據(jù)。 第三章體細(xì)胞突變和甲基化在腫瘤發(fā)生過程中起著重要作用。本文設(shè)計(jì)了通過基因分析非侵入性檢測膀胱癌的標(biāo)志物,該方法包括體細(xì)胞突
[Abstract]:Bladder cancer is a common cancer in human urinary system. Noninvasive early detection of bladder cancer is of great significance in clinical diagnosis and has been widely studied.In this paper, we studied the capture of exfoliated cells in urine and the detection of tumor-related genes in order to improve the efficiency of cell capture and enhance the sensitivity of detection. We hope to be used in the early clinical diagnosis or prognosis of bladder cancer.The main contents and conclusions of the study are as follows:In the first chapter, the methods of isolation and capture of tumor cells from body fluid are reviewed, and the methods and recent progress of early detection of bladder cancer are described in detail.On the basis of this, the paper's research ideas and schemes are put forward.In Chapter 2, the tumor cells fall off into urine during the development of bladder cancer, which provides the possibility for noninvasive detection of bladder cancer.In this paper, polycarbonate microporous membrane and nickel microporous screen were used to capture bladder cancer cells in urine rapidly and efficiently, and the characteristics of dichloromethane membrane were used to recover the cells captured on polycarbonate microporous filter membrane.The micropore filtration method was superior to the traditional centrifugation and enrichment method in capturing cell efficiency and separating and enriching cells for 20 min.The effects of filter membrane type, fixation agent and flow rate on the capture efficiency of tumor cells were investigated systematically in this experiment. The method was fast, low cost and environmentally friendly.It provides the possibility and theoretical basis for clinical diagnosis in the future.Chapter 3 somatic mutation and methylation play an important role in tumorigenesis.A noninvasive marker for the detection of bladder cancer by gene analysis has been designed. This method includes somatic processes.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.14

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 陳萬青;張思維;曾紅梅;鄭榮壽;鄒小農(nóng);趙平;吳良有;李光琳;赫捷;;中國2010年惡性腫瘤發(fā)病與死亡[J];中國腫瘤;2014年01期

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本文編號：1719440