本文選題：前列腺癌抗原3　切入點：前列腺癌　出處：《山西醫(yī)科大學》2017年碩士論文

【摘要】：目的:研究外周血前列腺癌抗原3(PCA3)在前列腺特異性抗原(PSA)灰區(qū)(4-10ng/ml)時對前列腺癌的診斷價值;通過測定PCA3在前列腺癌不同病理分級患者中的表達量研究其與前列腺癌病理分級(分化程度)的關系。方法:隨機選取2016年4月至2016年12月間山西醫(yī)科大學第一醫(yī)院泌尿外科住院的前列腺癌患者30例作為試驗組,另選取良性前列腺增生患者34例作為對照組,并且其血PSA均為4-10ng/ml。采用實時熒光PCR技術檢測兩組外周血單個核細胞中PCA3 m RNA相對表達量,并統(tǒng)計前列腺癌組PCA3 m RNA相對表達量與高分化(Gleason評分2-4分)、中分化(Gleason評分5-6分)、低分化(Gleason評分7-10分)前列腺癌的關系。結果:在64例受試者中,30例經病理診斷為前列腺癌,其PCA3的相對表達量為2.50±1.79;34例為良性前列腺增生,其PCA3的相對表達量為1.01±0.54,P0.05,差異有統(tǒng)計學意義。表明PCA3 m RNA高表達于前列腺癌患者血液,在良性前列腺增生患者中低表達。30例前列腺癌患者中,8例Gleason評分為7-10分,其PCA3的相對表達量為4.40±2.27;17例Gleason評分為5-6分,其PCA3的相對表達量為2.13±0.76;5例Gleason評分為2-4分,其PCA3的相對表達量為0.72±0.28,三組之間兩兩相比差異均有統(tǒng)計學意義,表明低分化組前列腺癌患者PCA3 m RNA相對表達量高于中分化組及高分化組前列腺癌的患者,中分化組前列腺癌患者PCA3 m RNA相對表達量高于高分化組前列腺癌的患者。結論:在PSA處于灰區(qū)的患者中,PCA3 m RNA相對表達量越高,患前列腺癌可能性越大,可為前列腺癌篩查提供參考,穿刺活檢陽性率高;在PSA處于灰區(qū)的前列腺癌患者中,PCA3 m RNA相對表達量越高,前列腺癌病理分級越高,前列腺癌細胞惡性程度越大,分化程度越低,提示預后較差。
[Abstract]:Objective: to study the diagnostic value of peripheral blood prostate cancer antigen (PCA3) in the prostate specific antigen (PSA) gray region (4-10 ng / ml). The relationship between the expression of PCA3 and the pathological grade (differentiation) of prostate cancer was studied by measuring the expression of PCA3 in patients with different pathological grades of prostate cancer. Methods: the first one of Shanxi Medical University from April 2016 to December 2016 was randomly selected. Thirty patients with prostate cancer in urology department were treated as trial group. 34 patients with benign prostatic hyperplasia (BPH) were selected as control group and their blood PSA was 4-10 ng / ml. The relative expression of PCA3 m RNA in peripheral blood mononuclear cells was detected by real-time fluorescent PCR technique. The relationship between the relative expression of PCA3 m RNA and Gleason score 2-4, Gleason score 5-6, Gleason score 7-10) was analyzed. Results: 30 of 64 subjects were pathologically diagnosed as prostate cancer. The relative expression of PCA3 was 2.50 鹵1.79m in 34 patients with benign prostatic hyperplasia, and the relative expression of PCA3 was 1.01 鹵0.54g P0.05.The results showed that PCA3 m RNA was highly expressed in the blood of patients with prostate cancer. The Gleason score was 7-10 in 8 patients with benign prostatic hyperplasia, the relative expression of PCA3 was 5-6 in 17 patients with Gleason, and the relative expression of PCA3 was 2.13 鹵0.760.The Gleason score was 2-4 in 5 patients with benign prostatic hyperplasia. The relative expression of PCA3 was 0.72 鹵0.28, the difference between the three groups was statistically significant, which indicated that the relative expression of PCA3 m RNA in the low differentiated prostate cancer patients was higher than that in the moderately differentiated prostate cancer patients and the well-differentiated prostate cancer patients. The relative expression of PCA3 m RNA in moderately differentiated prostate cancer patients was higher than that in well differentiated prostate cancer patients. Conclusion: the higher the relative expression of PCA3 m RNA in patients with PSA in gray zone, the more likely it is to develop prostate cancer. The higher the relative expression of PCA3 m RNA, the higher the pathological grade of prostate cancer, and the greater the malignant degree of prostate cancer cells, the higher the positive rate of puncture biopsy in prostate cancer screening, the higher the relative expression of PCA3 m RNA in prostate cancer patients in the gray area of PSA, and the higher the pathological grade of prostate cancer is. The lower the degree of differentiation, the worse the prognosis.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.25

【參考文獻】

相關期刊論文 前6條

1 袁利榮;張承廣;魯來興;阮磊;蘭建宏;馮森強;羅金旦;;經會陰及經直腸前列腺穿刺活檢術的臨床應用分析[J];中華男科學雜志;2014年11期

2 韓蘇軍;張思維;陳萬青;李長嶺;;中國前列腺癌發(fā)病現狀和流行趨勢分析[J];臨床腫瘤學雜志;2013年04期

3 蘆志華;朱生才;朱剛;馬宏;萬奔;王建業(yè);;經會陰和經直腸途徑前列腺穿刺活檢并發(fā)癥的比較分析[J];臨床泌尿外科雜志;2008年05期

4 張元芳;;重視前列腺癌診斷和治療的研究[J];臨床外科雜志;2008年02期

5 翁志梁;余凱遠;毛曉露;陶志華;陳曉東;吳秀玲;胡元平;王思齊;李澄棣;陳占國;;外周血差異顯示編碼3mRNA定量檢測在前列腺癌患者診斷與治療監(jiān)測中的初步應用[J];中華醫(yī)學雜志;2006年41期

6 鐘晨陽,李榮強,陳敏,王文超,李颯英,鄧京平,魏東,王建業(yè),萬奔;臨床參數和磁共振成像預測前列腺癌病理分期的評價[J];中華泌尿外科雜志;2005年01期

，

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