本文選題：亞硝酸鹽　切入點(diǎn)：生殖毒性　出處：《解剖學(xué)報(bào)》2017年05期

【摘要】：目的探討亞硝酸鹽暴露對(duì)雄性小鼠生殖毒性的分子機(jī)制。方法 36只2月齡健康雄性小鼠,隨機(jī)分為對(duì)照組(生理鹽水)、低劑量組(60 mg/kg)和高劑量組(120 mg/kg),每組12只進(jìn)行亞硝酸鹽灌胃3個(gè)月,觀察小鼠的生長(zhǎng)狀況,HE染色法觀察睪丸組織病理變化,免疫熒光和Western blotting方法分析檢測(cè)睪丸組織細(xì)胞增殖與凋亡情況及DNA甲基化、組蛋白去乙�；嚓P(guān)酶的表達(dá)情況。結(jié)果亞硝酸鹽暴露組小鼠較對(duì)照組小鼠體重增加緩慢,睪丸指數(shù)降低(P0.01),形態(tài)發(fā)生病理性改變;亞硝酸鹽暴露組小鼠睪丸組織細(xì)胞增殖較對(duì)照組明顯減少,細(xì)胞凋亡較對(duì)照組明顯增加(P0.01);同時(shí)DNA甲基化和組蛋白去乙�；礁哂趯�(duì)照組(P0.01),且均具有劑量依賴性。結(jié)論亞硝酸鹽暴露通過(guò)抑制雄性小鼠生長(zhǎng)發(fā)育及睪丸生精細(xì)胞增殖,誘導(dǎo)睪丸生精細(xì)胞凋亡,造成雄性生殖毒性;DNA甲基化及組蛋白去乙酰化水平升高,提示表觀遺傳學(xué)可能參與了亞硝酸鹽暴露對(duì)雄性生殖系統(tǒng)的損傷過(guò)程及調(diào)控機(jī)制。
[Abstract]:Objective to explore the molecular mechanism of reproductive toxicity of nitrite exposure in male mice. They were randomly divided into two groups: control group (normal saline group), low dose group (60 mg / kg) and high dose group (120 mg / kg). 12 rats in each group were treated with nitrite for 3 months. The growth of mice was observed by HE staining method and the pathological changes of testicular tissue were observed. The proliferation and apoptosis of testicular cells and the expression of DNA methylation and histone deacetylation related enzymes were detected by immunofluorescence and Western blotting. Results the weight gain of nitrite exposed mice was slower than that of control mice. The testicular index decreased P0.01G and morphogenetic pathological changes, the proliferation of testicular cells in the nitrite exposed group was significantly lower than that in the control group. The levels of DNA methylation and histone deacetylation were higher than those of the control group in a dose-dependent manner. Conclusion nitrite exposure can inhibit the growth and development of male mice and the proliferation of spermatogenic cells. The apoptosis of testicular spermatogenic cells was induced and the DNA methylation and histone deacetylation level of male reproductive toxicity were increased, which suggested that epigenetics might be involved in the damage process and regulation mechanism of male reproductive system induced by nitrite exposure.
【作者單位】： 鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院人體解剖學(xué)系;河南大學(xué)神經(jīng)生物學(xué)研究所;
【基金】：高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金(20134101110014)
【分類號(hào)】：R698.2
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本文編號(hào)：1678902