本文選題：前列腺癌　切入點：垂體腫瘤轉(zhuǎn)化基因　出處：《中華男科學(xué)雜志》2017年07期 　論文類型：期刊論文

【摘要】：目的:探討下調(diào)垂體腫瘤轉(zhuǎn)化基因1(PTTG1)表達(dá)對雄激素非依賴性前列腺癌LNCa P-AI細(xì)胞增殖、侵襲、凋亡,以及對雄激素拮抗劑敏感性的影響。方法:LNCa P-AI細(xì)胞轉(zhuǎn)染靶向PTTG1基因的siRNA,MTT法檢測細(xì)胞增殖,Transwell法檢測細(xì)胞侵襲,流式細(xì)胞術(shù)檢測細(xì)胞凋亡,Western印跡檢測PTTG1、p-Akt、p-ERK等蛋白表達(dá)水平,瓊脂糖凝膠電泳法檢測PTTG1 mRNA表達(dá)水平。結(jié)果:siRNA有效抑制了PTTG1的表達(dá);下調(diào)PTTG1表達(dá)有效抑制了LNCa P-AI細(xì)胞在去雄激素培養(yǎng)液中的增殖,24、48、72 h抑制率分別為(19.47±2.12)%、(24.01±2.13)%、(48.02±2.22)%,組間比較有顯著性差異(P0.05);降低其侵襲力,Transwell試驗顯示,對照組、轉(zhuǎn)染24、48、72 h后穿過聚碳酸酯膜細(xì)胞個數(shù)分別為111.11±13.47、74.67±9.85、56.44±8.66、37.33±6.14,組間比較有顯著性差異(P0.01);siRNA-PTTG1轉(zhuǎn)染LNCa P-AI細(xì)胞后,空白對照組、轉(zhuǎn)染24、48、72 h后凋亡率分別為(2.17±0.49)%、(18.32±0.94)%、(19.94±1.30)%、(21.73±1.88)%,各組間比較有顯著性差異(P0.05)。siRNA聯(lián)合氟他胺組對LNCa P-AI增殖的抑制作用和促凋亡作用顯著強于siRNA或者氟他胺組,并呈氟他胺濃度相關(guān)性;50 nmol/L氟他胺、siRNA聯(lián)合50 nmol/L氟他胺對LNCa P-AI的抑制率分別為(27.13±3.52)%、(67.51±5.13)%,兩組間比較有顯著性差異(P0.05);凋亡率分別為(3.94±0.48)%、(19.93±1.72)%,兩組間比較有顯著性差異(P0.05);100 nmol/L氟他胺、siRNA聯(lián)合100 nmol/L氟他胺的抑制率分別為(43.72±3.90)%、(73.19±4.78)%,兩組間比較有顯著性差異(P0.05);凋亡率分別為(5.33±0.66)%、(23.43±1.76)%,兩組間比較有顯著性差異(P0.05)。結(jié)論:siRNA下調(diào)PTTG1表達(dá)能夠抑制LNCa P-AI的增殖和侵襲,促進(jìn)凋亡,提高了LNCa P-AI細(xì)胞對雄激素拮抗劑氟他胺的敏感性,抑制PTTG1表達(dá)可能會強化雄激素剝奪治療晚期前列腺癌的作用。
[Abstract]:Objective: To investigate the downregulation of pituitary tumor transforming gene 1 (PTTG1) expression on the proliferation of androgen independent prostate cancer LNCa P-AI cells, invasion, apoptosis, and the effect of androgen antagonist sensitivity. Methods: siRNA LNCa cells were transfected with P-AI targeting PTTG1 gene, MTT cell proliferation assay, cell invasion was detected by Transwell assay. Flow cytometry, PTTG1 p-Akt, Western blot, the expression level of p-ERK protein, detected the expression of PTTG1 mRNA by agarose gel electrophoresis. Results: siRNA inhibited the expression of PTTG1 down regulated PTTG1 expression; effective suppression of LNCa in P-AI cells to androgen in the culture medium of proliferation, the inhibition rate of H 24,48,72 respectively (19.47 + 2.12)% and (24.01 + 2.13)% and (48.02 + 2.22)%, there were significant difference between two groups (P0.05); reduce the invasion force, Transwell test showed that the control group, h after transfection of 24,48,72 through poly carbon 閰擱叝鑶滅粏鑳?yōu)涓暟鍒嗗埆湄?fù)111.11鹵13.47,74.67鹵9.85,56.44鹵8.66,37.33鹵6.14,緇勯棿姣旇緝鏈夋樉钁楁，

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