本文選題：膀胱移行細(xì)胞癌　切入點(diǎn)：熒光原位雜交　出處：《吉林大學(xué)》2014年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的：利用熒光原位雜交技術(shù)，測(cè)出膀胱移行細(xì)胞癌患者尿脫落細(xì)胞中3號(hào)染色體、7號(hào)染色體、17號(hào)染色體及9號(hào)染色體p16基因突變值，探討突變值與膀胱移行細(xì)胞癌病理分級(jí)之間是否存在相關(guān)性，為膀胱移行細(xì)胞癌的早期診斷及分級(jí)診斷提供一種新的方法。 方法： 1、選取實(shí)驗(yàn)對(duì)象100例（2012年10月至2013年5月期間吉林大學(xué)中日聯(lián)誼醫(yī)院確診膀胱占位性病變，且脫落細(xì)胞檢測(cè)發(fā)現(xiàn)尿中存在脫落細(xì)胞）。2、進(jìn)行熒光原位雜交檢查，并記錄尿脫落細(xì)胞中3號(hào)染色體、7號(hào)染色體、17號(hào)染色體及9號(hào)染色體p16基因突變值。3、術(shù)中留取組織標(biāo)本（已獲得醫(yī)學(xué)倫理委員會(huì)認(rèn)可），HE染色后，行組織病理學(xué)檢查，確定腫瘤分級(jí)。分級(jí)標(biāo)準(zhǔn)采用WHO2004分級(jí)法。 結(jié)果：FISH檢測(cè)所有患者尿脫落細(xì)胞均可見(jiàn)有熒光標(biāo)記，且3號(hào)染色體、17號(hào)染色體異常細(xì)胞個(gè)數(shù)及3號(hào)染色體、7號(hào)染色體、17號(hào)染色體異常細(xì)胞內(nèi)非整倍染色體的個(gè)數(shù)（即突變值）與腫瘤病理分級(jí)呈正相關(guān)性(Sig＜0.005)； 結(jié)論：3號(hào)、17號(hào)染色體異常細(xì)胞個(gè)數(shù)及3號(hào)、7號(hào)、17號(hào)染色體異常細(xì)胞內(nèi)非整倍染色體的個(gè)數(shù)（即突變值）與膀胱移行細(xì)胞癌病理分級(jí)呈正相關(guān)性，9號(hào)染色體p16基因的丟失與病理分級(jí)之間無(wú)明顯相關(guān)性�？烧J(rèn)為熒光原位雜交技術(shù)檢測(cè)能夠區(qū)分不同病理級(jí)別的膀胱移行細(xì)胞癌，，可用于臨床早期病理分級(jí)診斷。
[Abstract]:Objective: to detect the mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells of patients with transitional cell carcinoma of bladder by fluorescence in situ hybridization. To explore the correlation between mutation value and pathological grade of bladder transitional cell carcinoma (TCC), and to provide a new method for early diagnosis and grading diagnosis of TCC. Methods:. 1. From October 2012 to May 2013, 100 patients (from October 2012 to May 2013) were selected to confirm the bladder occupying lesions, and the exfoliated cells were detected by fluorescence in situ hybridization, and the exfoliated cells were found to exist in the urine. The mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells was recorded. The grade of tumor was determined. The standard of classification was WHO2004. Results the urine exfoliated cells of all the patients were detected with fluorescent markers by the urine sample. The number of abnormal cells on chromosome 3 and 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 were positively correlated with pathological grade of tumor. Conclusion: the number of abnormal cells on chromosome 3, 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 are positively correlated with the pathological grade of bladder transitional cell carcinoma. There is no significant correlation between loss and pathological grade. Fluorescence in situ hybridization can be used to distinguish transitional cell carcinoma of bladder with different pathological grades. It can be used for clinical early pathological grading diagnosis.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R737.14

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