本文關(guān)鍵詞： 微小RNA 腎透明細(xì)胞癌 基因 Bcl-　出處：《重慶醫(yī)學(xué)》2015年15期 　論文類型：期刊論文

【摘要】：目的研究微小RNA204(miR-204)在人腎透明細(xì)胞癌(RCCC)中的表達(dá)及臨床意義。方法收集泌尿外科行手術(shù)切除的RCCC及對(duì)應(yīng)癌旁正常腎臟組織標(biāo)本共65例,運(yùn)用qRT-PCR技術(shù)檢測(cè)miR-204在RCCC及癌旁組織中的表達(dá)水平,統(tǒng)計(jì)分析miR-204表達(dá)水平與患者臨床病理資料間的相關(guān)性;采用人工合成的miR-204模擬物轉(zhuǎn)染人RCCCCaki-1細(xì)胞,分別采用qRT-PCR及Western-Blot檢測(cè)轉(zhuǎn)染后miR-204下游潛在靶點(diǎn)Bcl-2及SIRT1 mRNA及蛋白的表達(dá)變化。結(jié)果miR-204在RCCC組織中表達(dá)水平顯著低于對(duì)應(yīng)癌旁正常腎組織(P0.05);miR-204低表達(dá)與腫瘤體積增大(3cm,P0.05)、腫瘤淋巴結(jié)轉(zhuǎn)移(P0.05)及高TNM分期(Ⅲ+Ⅳ期,P0.05)顯著相關(guān);miR-204轉(zhuǎn)染人RCCCCaki-1細(xì)胞后可顯著降低細(xì)胞內(nèi)Bcl-2及SIRT1mRNA及蛋白的表達(dá)水平(P0.05)。結(jié)論 miR-204在RCCC組織中表達(dá)下調(diào)并與腫瘤惡性臨床病理特征有關(guān),miR-204可能通過(guò)下調(diào)Bcl-2及SIRT1的表達(dá)來(lái)抑制RCCC的發(fā)生、發(fā)展過(guò)程。
[Abstract]:Objective to study the expression and clinical significance of minimal RNA204miR-204 in human renal clear cell carcinoma (RCCC). Methods 65 cases of RCCC and adjacent normal renal tissues were collected from urological surgery. QRT-PCR technique was used to detect the expression of miR-204 in RCCC and adjacent tissues, and the correlation between miR-204 expression and clinical and pathological data of patients was analyzed statistically. Human RCCCCaki-1 cells were transfected with synthetic miR-204 mimics. QRT-PCR and Western-Blot were used to detect the expression of Bcl-2, SIRT1 mRNA and protein downstream of miR-204. Results the expression of miR-204 in RCCC tissues was significantly lower than that in normal renal tissues adjacent to cancer. The expression level of Bcl-2, SIRT1mRNA and protein in human RCCCCaki-1 cells was significantly decreased after transfection of p0.05) and high TNM stage (鈪，

本文編號(hào)：1544753