本文關鍵詞： 小鼠 腎積水 缺氧誘導因子-1α 血管內皮生長因子 microRNA-210 UUO　出處：《南昌大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的和意義： 腎積水是多種腎內外因素作用于泌尿系后引起的一種常見的臨床表現(xiàn)。如果引起腎積水的因素不能得到及時解除，腎臟皮質會因為受到尿液壓迫而引起腎組織的缺血缺氧，因此盡快解除引起腎積水的因素對于腎功能的保護有著極為重要的意義。研究發(fā)現(xiàn)新生血管的發(fā)生發(fā)展主要通過VEGF信號通路來發(fā)揮調控功能。最近研究報道發(fā)現(xiàn)miRNA可以調控血管新生，缺血缺氧后組織中HIF-1α表達上調，某些miRNA表達也會發(fā)生顯著的改變。研究還提示miRNA-210這一作用可能是通過調控下游基因參與血管生成的信號通路并以此影響血管生成。 本研究通過制作小鼠單側腎積水病理模型，觀察小鼠腎積水后缺氧誘導因子-1α(HIF-1α)、血管內皮生長因子（VEGF）、microRNA-210在不同時間點的的表達變化并探討其調控機制。 方法： 采用絲線結扎單側輸尿管的方法制備急性完全性單側腎積水病理模型（UUO模型），分為假手術對照組和單側腎積水組，于單側腎積水后2d、5d、9d、14d分別頸椎脫臼處死小鼠收集患腎。采用熒光實時定量PCR的方法檢測單側腎積水后患腎各個時間點HIF-1mRNA、VEGFmRNA及microRNA-210的表達變化，采用western-blot方法檢測單側腎積水后各時間點HIF-1α蛋白的表達變化趨勢。 結果： HIF-1αmRNA表達水平在腎積水后表達量逐漸上調直到處死該實驗動物（P均＜0.05）。VEGFmRNA及microRNA-210表達水平在單側腎積水后2d升高并達到高峰，于單側腎積水后5d、9d、14d表達量逐漸下調（P均＜0.05）。HIF-1α蛋白表達量逐漸上調。 結論： 實驗結果表明單側腎積水后患腎HIF-1αmRNA及蛋白表達逐步上調，VEGFmRNA及microRNA-210表達出現(xiàn)一過性上調，可能與單側腎積水后患腎皮質被尿液壓迫，，機體對缺氧、缺血產生適應性反應有一定關系。
[Abstract]:Purpose and significance:
Hydronephrosis is a common clinical manifestation of renal function of various internal and external factors in the urinary system caused by hydronephrosis caused by factors. If not be lifted in time because of renal cortex caused by compression of urine kidney tissue ischemia and hypoxia, so as soon as possible to lift the protection factors to cause hydronephrosis and renal function has a very important. The study found that the development of new blood vessels mainly through the VEGF pathway to play a regulatory function. Recent studies suggest that miRNA can regulate angiogenesis, upregulation of HIF-1 expression after ischemia and hypoxia, some miRNA expression will also change significantly. This study also suggests a possible role of miRNA-210 signaling pathway is involved in blood vessel generated by the regulation of downstream genes and thus affect angiogenesis.
In this study, we made a pathological model of unilateral hydronephrosis in mice, and observed the expression changes of hypoxia inducible factor -1 alpha (HIF-1 alpha), vascular endothelial growth factor (VEGF) and microRNA-210 at different time points after hydronephrosis in mice, and discussed the regulation mechanism.
Method錛

本文編號：1526334