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  本文關(guān)鍵詞：腺病毒介導(dǎo)的PDCD4基因過表達(dá)促進(jìn)腎癌BTG1蛋白的表達(dá)和786-O細(xì)胞的凋亡　出處：《中國老年學(xué)雜志》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 腎癌 PDCD 腺病毒 -O

【摘要】：目的探討過表達(dá)抑癌基因PDCD4的腺病毒對腎透明細(xì)胞腺癌細(xì)胞株(786-O)的影響及腎臟注射后對腎癌的治療效果。方法體外構(gòu)建過表達(dá)抑癌基因PDCD4的腺病毒;培養(yǎng)786-O細(xì)胞株,轉(zhuǎn)染腺病毒體外干預(yù),觀察細(xì)胞的死亡情況;將SD大鼠隨機(jī)分為對照組和移植組,兩組均在前肢腋部皮下注射786-O細(xì)胞株構(gòu)建腎癌模型,造模14 d后,對照組瘤中注射0.1 ml D-Hanks,移植組則注射含1×10~(11)vp/m L的表達(dá)抑癌基因PDCD4的腺病毒的D-Hanks 0.1 ml。結(jié)果體外觀察轉(zhuǎn)染病毒后,786-O細(xì)胞的生長活力下降,死亡率升高;體內(nèi)注射病毒后,可以觀察到腎臟中抑癌基因PDCD4的上調(diào)和BTG1蛋白表達(dá)水平的升高。結(jié)論過表達(dá)抑癌基因PDCD4的腺病毒注射治療腎癌具有可行性。
[Abstract]:Objective to investigate the effect of adenovirus overexpression of tumor suppressor gene PDCD4 on renal clear cell adenocarcinoma cell line 786-O). Methods Adenoviruses expressing tumor suppressor gene PDCD4 were constructed in vitro. 786-O cell line was cultured and transfected with adenovirus in vitro to observe the cell death. SD rats were randomly divided into two groups: control group and transplantation group. Both groups were injected with 786-O cell line subcutaneously into the armpit of forelimb to construct renal cell carcinoma model, and the model was made 14 days later. In the control group, 0.1 ml D-Hanks were injected into the tumor. The transplanted group was injected with D-Hanks 0.1 ml of adenovirus containing 1 脳 10 ~ (-1) v / m L PDCD4. Results after transfection of the virus in vitro, the expression of tumor suppressor gene PDCD4 was observed. The growth activity of 786-O cells decreased and the mortality increased. After the virus was injected into the body. The upregulation of tumor suppressor gene PDCD4 and the increase of BTG1 protein expression can be observed in kidney. Conclusion it is feasible to treat renal cell carcinoma with adenovirus injection with overexpression of tumor suppressor gene PDCD4.
【作者單位】： 金華市人民醫(yī)院泌尿外科;
【基金】：金華市科學(xué)技術(shù)局資助課題(No.2013-3-057)
【分類號】：R737.11
【正文快照】： 加深對腎癌的深層認(rèn)知,從基因水平尋找突破口將是治療腎癌有效的途徑〔1,2〕。程序化凋亡因子(PDCD)4是一種抑癌基因,能夠通過啟動e IF4A、e IF4G達(dá)到抑制細(xì)胞生長的效果。癌組織中的PDCD4表達(dá)下降甚至缺失,提示我們上調(diào)PDCD4表達(dá)可能是啟動癌細(xì)胞凋亡的分子開關(guān)〔3〕。腺病毒

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