目的:探討右美托咪定通過p38通路改善發(fā)育期大鼠七氟醚麻醉后認(rèn)知功能障礙。方法:將90只發(fā)育期大鼠根據(jù)隨機(jī)數(shù)字法分為對照組（C組）、七氟醚組（S組）、七氟醚+右美托咪定組（S+D組）,每組30只。缺口末端標(biāo)記法（TUNEL）染色測定大鼠海馬CA1區(qū)凋亡細(xì)胞數(shù),免疫熒光染色測定大鼠海馬CA1區(qū)Caspase-3、B淋巴細(xì)胞瘤-2基因（Bcl-2）及相關(guān)的蛋白（Bax）表達(dá),免疫組化染色測定大鼠海馬CA1區(qū)p38絲裂原活化蛋白激酶（p38MAPK）表達(dá),采用Western blot測定海馬CA1區(qū)p38MAPK、磷酸化p38MAPK（p-p38MAPK）蛋白水平,Morris水迷宮實(shí)驗(yàn)測定認(rèn)知功能。結(jié)果:與C組比較,S組和S+D組大鼠海馬CA1區(qū)凋亡細(xì)胞數(shù)升高（P<0.05）,海馬Caspase-3、Bax陽性細(xì)胞數(shù)和熒光強(qiáng)度升高（P<0.05）,Bcl-2陽性細(xì)胞數(shù)和熒光強(qiáng)度降低（P<0.05）,海馬CA1區(qū)p38MAPK免疫組化IHS評分升高（P<0.05）,海馬CA1區(qū)p-p38MAPK蛋白水平升高（P<0.05）,逃避潛伏期延長（P<0.05）,... 

【文章來源】：中國免疫學(xué)雜志. 2020,36(09)北大核心CSCD

【文章頁數(shù)】：6 頁

【部分圖文】：

大鼠海馬CA1區(qū)凋亡細(xì)胞TUNEL染色(×400)

表3 大鼠海馬CA1區(qū)p38MAPK免疫組化IHS評分比較 ( x ˉ ±s) Tab.3 Comparison of immunohistochemical IHS scores of p38MAPK in hippocampal CA1 region of rats ( x ˉ ±s) Groups n p38MAPK IHS scores(score) Group C 10 0.57±0.42 Group S 10 3.15±0.571) Group S+D 10 1.46±0.531)2) F 65.869 P 0.000 Note:Compared with group C,1)P<0.05;compared with group S,2)P<0.05.表4 大鼠海馬CA1區(qū)p38MAPK、p-p38MAPK蛋白水平比較 ( x ˉ ±s) Tab.4 Comparison of p38MAPK and p-p38MAPK protein levels in hippocampal CA1 region of rats ( x ˉ ±s) Groups n p38MAPK protein p-p38MAPK protein Group C 10 0.24±0.08 0.11±0.04 Group S 10 0.22±0.11 0.92±0.131) Group S+D 10 0.29±0.10 0.65±0.111)2) F 1.368 166.765 P 0.272 0.000 Note:Compared with group C,1)P<0.05;compared with group S,2)P<0.05.

表4 大鼠海馬CA1區(qū)p38MAPK、p-p38MAPK蛋白水平比較 ( x ˉ ±s) Tab.4 Comparison of p38MAPK and p-p38MAPK protein levels in hippocampal CA1 region of rats ( x ˉ ±s) Groups n p38MAPK protein p-p38MAPK protein Group C 10 0.24±0.08 0.11±0.04 Group S 10 0.22±0.11 0.92±0.131) Group S+D 10 0.29±0.10 0.65±0.111)2) F 1.368 166.765 P 0.272 0.000 Note:Compared with group C,1)P<0.05;compared with group S,2)P<0.05.表5 大鼠水迷宮結(jié)果比較 ( x ˉ ±s) Tab.5 Comparison of water maze results of rat ( x ˉ ±s) Groups n Escape latency(s) Number of crossing theoriginal platform(times) 1 d 2 d 3 d 4 d Group C 10 26.41±3.52 15.46±3.38 12.16±2.28 9.42±2.35 8.79±1.74 Group S 10 42.25±3.411) 33.57±3.521) 28.72±2.311) 25.47±2.461) 4.35±1.581) Group S+D 10 34.17±3.261)2) 22.15±3.371)2) 19.03±2.161)2) 15.71±2.171)2) 6.21±1.641)2) F 54.322 71.527 136.620 120.501 18.159 P 0.000 0.000 0.000 0.000 0.000 Note:Compared with group C,1)P<0.05;compared with group S,2)P<0.05.

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