丙泊酚對(duì)小腦皮層浦肯野細(xì)胞自發(fā)性活動(dòng)的影響[目的]丙泊酚,2,6-雙（1-甲基乙基）苯酚乳劑,是一種快速靜脈鎮(zhèn)靜催眠藥,廣泛用于麻醉的誘導(dǎo)與維持,也用于特護(hù)病房（ICU）患者的鎮(zhèn)靜。丙泊酚對(duì)整個(gè)中樞神經(jīng)系統(tǒng)均有抑制作用,其最常見(jiàn)副作用是劑量依存地抑制心肺功能。已證實(shí)丙泊酚具有活化γ氨基丁酸（GABA）A受體、抑制N-甲基-D-天門冬氨酸（NMDA）受體,并通過(guò)慢速鈣離子通道調(diào)節(jié)鈣內(nèi)流。在離體條件下,丙泊酚可通過(guò)活化GABAA和甘氨酸受體對(duì)脊髓、海馬和下丘腦神經(jīng)元的活動(dòng)產(chǎn)生抑制作用,然而,丙泊酚對(duì)活體動(dòng)物小腦皮層神經(jīng)元活動(dòng)的影響尚不清楚。因此,我們應(yīng)用細(xì)胞貼附式記錄和藥理學(xué)手段研究丙泊酚對(duì)烏拉坦麻醉小鼠浦肯野細(xì)胞自發(fā)性活動(dòng)的影響,旨在闡明靜脈麻醉藥丙泊酚對(duì)小腦皮層浦肯野細(xì)胞自發(fā)性單純性放電和復(fù)雜性放電的影響機(jī)制。[方法]成年ICR小鼠（6-8周齡）通過(guò)腹腔注射烏拉坦麻醉（1.3g/kg體重）后,行氣管插管以保證呼吸道暢通,然后將動(dòng)物固定在自制的腦立體定位儀上。在小腦部位制作一個(gè)灌流槽后,在小腦CrusⅡ區(qū)相對(duì)應(yīng)處行開(kāi)顱手術(shù),鉆一個(gè)直徑約為1-1.5mm小孔,暴露記錄部位的小腦表面,輕輕去... 

【文章來(lái)源】：延邊大學(xué)吉林省 211工程院校

【文章頁(yè)數(shù)】：88 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
Abbreviations
Chapter 1.Effect of propofol on spontaneous activity of cerebellar Purkinje cells in vivo inmice
    Abstract
    摘要
    1.1 Introduction
    1.2 Materials and methods
        1.2.1 Animals and Anesthesia
        1.2.2 Experiment Equipment
        1.2.3 Surgical procedures
        1.2.4 Electrophysiological recordings
        1.2.5 Drug administration
        1.2.6 Statistical analysis
    1.3 Results
        1.3.1 Cerebellar surface application of propofol depressed SS firing rate of PCs
A and GABA_B receptors activity did not prevented thepropofol-induced depression of PC SS firing rate">        1.3.2 Blockade of GABA_A and GABA_B receptors activity did not prevented thepropofol-induced depression of PC SS firing rate
A receptors activity abolished thepropofol-induced depression of PC SS firing rate">        1.3.3 Simultaneously blocking glycine and GABA_A receptors activity abolished thepropofol-induced depression of PC SS firing rate
    1.4 Discussion
        1.4.1 Propofol and GABAergic transmission
Aand glycine receptors activity">        1.4.2 Propofol depressed cerebellar PC SS firing rate via activation of both GABA_Aand glycine receptors activity
    1.5 Conclusion
    References
Chapter 2.Effect of propofol on sensory information transmission of cerebellar Purkinjecells in vivo in mice
    Abstract
    摘要
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Animals and Anesthesia
        2.2.2 Experiment Equipment
        2.2.3 Surgical procedures
        2.2.4 Electrophysiological recordings
        2.2.5 Drug administration
        2.2.6 Statistical analysis
    2.3 Results
        2.3.1 Effects of propofol on the sensory stimulation evoked responses of PCs
A receptor activity">        2.3.2 Effects of propofol on facial stimulation evoked responses in cerebellar PCs inthe absence of GABA_A receptor activity
    2.4 Discussion
        2.4.1 Propofol inhibited sensorry stimulation evoked GABAergic responses of PCs
        2.4.2 Propofol enhanced the sensory stimulation evoked parallel fiber-PCs excitatorytransmission
    2.5 Conclusions
    References
Review:Effects of propofol on central nervous system
    References
Publication
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