[目的]N-甲基-D-天冬氨酸受體是離子型谷氨酸受體,它參與神經(jīng)元突觸后反應(yīng)以及調(diào)控突觸前神經(jīng)遞質(zhì)的釋放。在中樞神經(jīng)系統(tǒng)中,它對突觸傳遞的調(diào)控起著關(guān)鍵作用。眾所周知,NMDA受體在哺乳動物的小腦皮層、顆粒細(xì)胞膜、平行纖維以及分子層中間神經(jīng)元上廣泛表達。在離體條件下,NMDA受體的活化增強浦肯野細(xì)胞的抑制性突觸后電流。然而,在在體條件下,NMDA對小腦神經(jīng)元的活動影響還不清楚。因此,在我們的實驗中,應(yīng)用電生理膜片鉗記錄和藥理學(xué)方法,探索外源性NMDA對烏拉坦麻醉小鼠小腦皮層浦肯野細(xì)胞自發(fā)性活動的影響,了解NMDA對小鼠小腦皮層浦肯野細(xì)胞自發(fā)性簡單峰電位放電的影響機制。[方法]實驗選取25只6-8周齡成年ICR小鼠,并對其進行腹腔注射烏拉坦麻醉（1.3 g/kg）。待40分鐘麻醉結(jié)束后,小鼠行氣管插管術(shù),使其呼吸道保持通暢,并將小鼠固定在腦立體定位裝置上。在小腦部位制作一個圓形的灌流槽,對小鼠小腦蚓部行開顱手術(shù),打孔直徑約為1-1.5 mm,暴露小腦表面的記錄區(qū)域,并輕輕地揭掉硬腦膜,使用蠕動泵在小腦表面持續(xù)不斷地灌流（0.4 ml/min）含95%02和5% CO2的人工腦脊液。用體溫維... 

【文章來源】：延邊大學(xué)吉林省 211工程院校

【文章頁數(shù)】：88 頁

【學(xué)位級別】：博士

【文章目錄】：
ABBREVIATION
Chapter 1 Effect of NMD A on spontaneous simple spikes of cerebellar Purkinje cellsin vivo in mice
    Abstract
    摘要
    1.1 Introduction
    1.2 Materials and Methods
        1.2.1 Animals and Anesthesia
        1.2.2 Experiment Equipment
        1.2.3 Surgical procedures
        1.2.4 Electrophysiological recordings
        1.2.5 Drug administration
        1.2.6 Statistical analysis
    1.3 Results
        1.3.1 Cerebellar surface application of NMDA decreased SS firing of PCs
A receptor">        1.3.2 NMDA reduced PC SS firing via the activation of GABA_A receptor
        1.3.3 Cerebellar surface application of NMDA evoked the excitation of MLIs
    1.4 Discussion
        1.4.1 NMDA onduced inhibition rather than excitation of cerebellar PCs
        1.4.2 NMDA mediated the excitation of cerebellar MLIs
        1.4.3 MLIs play a key rolr in controlling the output of PCs
    1.5 Conclusion
    References
Chapter 2 Effect of NMDA on spontaneous complex spikes of cerebellar Purkinjecells in vivo in mice
    Abstract
    摘要
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Animals and Anesthesia
        2.2.2 Experiment Equipment
        2.2.3 Surgical procedures
        2.2.4 Electrophysiological recordings
        2.2.5 Drug administration
        2.2.6 Statistical analysis
    2.3 Results
        2.3.1 Effects of NMDA on CS activity of PCs in the cerebellar cortex
        2.3.2 NMDA enhanced CS-evoked AHP currents
        2.3.3 Blockade of NMDAR attenuated the strength of spontaneous CS
        2.3.4 Blockade AMPARs abolished spontaneous CS firing and NMDARs activity
    2.4 Discussion
    2.5 Conclusions
    References
Review NMDA receptor and it's funcation in cerebellar cortex
    References
Publication
Acknowledgement



本文編號：3067861