【摘要】：目的研究刺五加皂苷對脊髓損傷后組織內(nèi)腦源性神經(jīng)營養(yǎng)因子(BDNF)和脊髓內(nèi)神經(jīng)生長因子(NGF)兩種蛋白表達的影響,探討刺五加皂苷對損傷脊髓的保護作用。方法將45只雄性大鼠隨機分為3組:假手術組、模型組和刺五加皂苷干預組,每組15只。將實驗動物用10%水合氯醛麻醉后,用改良Allen’s法進行急性脊髓損傷模型造模。造模后,假手術組予正常飼養(yǎng),模型組予連續(xù)7d生理鹽水腹腔注射,刺五加皂苷干預組進行連續(xù)7d100mg/kg刺五加皂苷持續(xù)腹腔注射。ELISA法檢測血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,通過病理切片分析大鼠脊髓組織病理變化,蛋白質(zhì)印跡法檢測大鼠脊髓內(nèi)BDNF和NGF表達。結果與假手術組比較,模型組和刺五加皂苷干預組脊髓有明顯損傷,且大鼠血清中MDA含量增加(P0.05),SOD活性降低(P0.05),大鼠脊髓組織中的BDNF和NGF蛋白表達量上升(P0.05)。與模型組相比,刺五加皂苷組大鼠脊髓損傷明顯改善,血清中MDA含量降低(P0.05),SOD活性升高(P0.05),大鼠脊髓組織中的BDNF和NGF蛋白表達量上升(P0.05)。結論刺五加皂苷促進脊髓組織中神經(jīng)元細胞內(nèi)BDNF和NGF表達,利于損傷神經(jīng)元的修復,對大鼠急性損傷的脊髓具有保護作用。
[Abstract]:Objective to study the effect of Acanthopanax senticosus saponins on the expression of brain-derived neurotrophic factor (BDNF) and spinal cord nerve growth factor (NGF) after spinal cord injury, and to explore the protective effect of Acanthopanax senticosus saponins on spinal cord injury. Methods 45 male rats were randomly divided into three groups: sham operation group, model group and Acanthopanax senticosus saponins intervention group, with 15 rats in each group. The experimental animals were anesthetized with 10% chloral hydrate and the model of acute spinal cord injury was established by modified Allen' s method. After the model was established, the model group was fed with normal saline for 7 days, and the intervention group was treated with continuous intraabdominal injection of Acanthopanax senticosus saponins. The content of malondialdehyde (MDA) and the activity of SOD (SOD) in serum were detected by Elisa, the pathological changes of spinal cord tissue were analyzed by pathological section, and the expression of BDNF and NGF in spinal cord of rats was detected by Western imprinting. Results compared with the pseudo-operation group, the spinal cord injury was obvious in the model group and Acanthopanax senticosus saponins intervention group, and the content of MDA in serum was increased (P 0.05), and the expression of BDNF and NGF protein in spinal cord tissue was increased (P 0.05). Compared with the model group, the spinal cord injury of rats in Acanthopanax senticosus saponins group was significantly improved, the content of MDA in serum was decreased (P 0.05), and the expression of BDNF and NGF protein in spinal cord tissue was increased (P 0.05). Conclusion Acanthopanax senticosus saponins promote the expression of BDNF and NGF in spinal cord tissue, which is beneficial to the repair of injured neurons and has protective effect on acute spinal cord injury in rats.
【作者單位】： 第二軍醫(yī)大學長海醫(yī)院骨科;
【分類號】：R651.2

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