【摘要】：目的：探討未成年SD大鼠行氯胺酮注射麻醉后對大鼠學(xué)習(xí)和記憶功能以及海馬即刻早期基因Egr-1表達(dá)的影響。 方法：選擇20只雄性SD未成年大鼠（21日齡），隨機分為2組，，每組10只，即氯胺酮（Ket）組、生理鹽水對照（Ns）組。未成年SD大鼠在實驗時間以外可以自由地取食和飲水。Ket組大鼠腹腔注射Ket50mg/kg （Ns稀釋至1ml），Ns組則給予等容量的生理鹽水，持續(xù)6天。在藥物注射期間注意觀察監(jiān)測并維持大鼠的生命體征正常。于給藥后24小時行Morris水迷宮定位航行實驗，測定其學(xué)習(xí)和記憶能力。于末次Morris水迷宮空間探索實驗結(jié)束后1h，腹腔注射麻醉劑（10%水合氯醛400mg/kg）后快速斷頭冰上取腦。采用實時熒光定量PCR法檢測未成年SD大鼠海馬Egr-1mRNA的表達(dá)情況，免疫印跡Western blotting法檢測海馬Egr-1蛋白的表達(dá)。 結(jié)果：（1）與Ns組比較， Ket組大鼠在定位航行和空間探索實驗中隱蔽平臺逃避潛伏期顯著延長（P 0.05）；活動總路徑縮短，穿越平臺次數(shù)明顯減少（P 0.05）。（2）兩實驗組海馬組織Egr-1mRNA均有一定量的表達(dá)，但與Ns組相比，Ket組Egr-1mRNA表達(dá)量下調(diào)，差異具有明顯的有統(tǒng)計學(xué)意義（P 0.05）。（3）Ket組大鼠海馬即刻早期基因Egr-1蛋白表達(dá)水平明顯低于Ns組（P 0.05）。 結(jié)論：未成年SD大鼠反復(fù)給予亞麻醉劑量的氯胺酮注射后，可降低大鼠在Morris水迷宮中的學(xué)習(xí)和記憶能力，并且同時也導(dǎo)致未成年SD大鼠海馬即刻早期基因Egr-1表達(dá)的降低。氯胺酮對未成年SD大鼠海馬即刻早期基因Egr-1表達(dá)的影響可能是其導(dǎo)致大鼠行為學(xué)改變的機制。
[Abstract]:Aim: to investigate the effects of ketamine injection anesthesia on learning and memory function and immediate early gene Egr-1 expression in hippocampus of juvenile SD rats. Methods: twenty male SD underage rats (21 days old) were randomly divided into two groups: ketamine (Ket) group (n = 10) and saline control group (Ns) group). The underage SD rats were free to eat and drink outside the experimental time. The rats in the Ket group were injected intraperitoneally with Ket50mg/kg (Ns diluted to 1ml), Ns group were given the same volume of saline for 6 days). Pay attention to observe, monitor and maintain the vital signs of rats during drug injection. The learning and memory ability of Morris water maze navigation experiment was measured 24 hours after administration. At the end of the last Morris water maze space exploration experiment, the brain was quickly decapitated on ice after intraabdominal injection of anesthetic (10% chloral hydrate 400mg/kg). The expression of Egr-1mRNA in hippocampus of juvenile SD rats was detected by real-time fluorescence quantitative PCR, and the expression of Egr-1 protein in hippocampus was detected by immunoblotting Western blotting. Results: (1) compared with Ns group, the escape latency of hidden platform in Ket group was significantly longer than that in Ket group. The expression of Egr-1mRNA in hippocampal tissue of both groups was significantly lower than that of Ns group, but the expression of Egr-1mRNA in Ket group was significantly lower than that in Ns group (P 0.05). (3). The expression of Egr-1 protein in hippocampus of Ket group was significantly lower than that of Ns group (P 0.05). Conclusion: repeated injection of ketamine into juvenile SD rats can decrease the learning and memory ability of rats in Morris water maze, and also lead to the decrease of immediate early gene Egr-1 expression in hippocampus of juvenile SD rats. The effect of ketamine on the expression of immediate early gene Egr-1 in hippocampus of juvenile SD rats may be the mechanism of behavioral changes in rats.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R614

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