【摘要】：目的：SD大鼠行星狀神經(jīng)節(jié)阻滯，觀察星狀神經(jīng)節(jié)阻滯對(duì)Langendorff離體心臟灌流裝置離體心功能的影響，為星狀神經(jīng)節(jié)阻滯用于臨床改善心功能減少圍手術(shù)期心血管事件發(fā)生提供理論依據(jù)。 方法：將24只8～10周齡體重180～220克的雄性SD大鼠采用隨機(jī)數(shù)字表法隨機(jī)分為3組，每組8只：(1)左側(cè)星狀神經(jīng)節(jié)阻滯組(LS組)；(2)右側(cè)星狀神經(jīng)節(jié)阻滯組(RS組)；(3)手術(shù)對(duì)照組（C組）。大鼠腹腔內(nèi)注射完成麻醉后，鈍性分開左右胸頭肌，沿食管旁找到星狀神經(jīng)節(jié)，埋管并縫皮，7天后行星狀神經(jīng)節(jié)阻滯實(shí)驗(yàn)，觀察是否出現(xiàn)霍納綜合征，并連續(xù)給藥兩周。星狀神經(jīng)節(jié)阻滯模型建立成功后，應(yīng)用Langendorff離體心臟灌流裝置建立SD大鼠離體心臟灌流模型。大鼠腹腔注射肝素后，仰臥位固定，迅速開胸，取出心臟，置于4℃冷的K-H液中分離主動(dòng)脈，懸掛于Langendorff離體心臟灌流裝置上。K-H液灌注15～20分鐘，達(dá)到平衡末時(shí)測(cè)定冠脈流量（CF），記錄相關(guān)的心功能指標(biāo)：心率（HR）左室發(fā)展壓（LVDP）左室舒張末壓（LVEDP）收縮期心室內(nèi)壓力升高速率（+dp/dt）舒張期心室內(nèi)壓力升高速率（-dp/dt）。同時(shí)取下心臟離心后取上清，經(jīng)過加標(biāo)準(zhǔn)品加樣加抗體洗滌以及加底物等步驟后，用ELISA法檢測(cè)心肌組織中腎上腺素（EPI）去甲腎上腺素（NE）神經(jīng)肽Y（NPY）和血管活性腸肽（VIP）的濃度。 結(jié)果：1.與C組相比，RSGB組LVDP+dp/dtmax-dp/dtmaxCF及HR值均升高有統(tǒng)計(jì)學(xué)意義(P＜0.05或0.01)，RSGB組的LVEDP值降低有統(tǒng)計(jì)學(xué)意義（P＜0.01）；與C組相比，LSGB組LVDP+dp/dtmax-dp/dtmax及CF值均升高有統(tǒng)計(jì)學(xué)意義(P＜0.05或0.01)，LSGB組的LVEDP值降低有統(tǒng)計(jì)學(xué)意義（P＜0.05）。與LSGB組相比，RSGB組LVDP+dp/dtmax及-dp/dtmax值升高有統(tǒng)計(jì)學(xué)意義（P＜0.05），RSGB組LVEDP值降低有統(tǒng)計(jì)學(xué)意義（P＜0.05）。2.與C組相比，RSGB組和LSGB組EPINENPY及VIP值均升高有統(tǒng)計(jì)學(xué)意義(P＜0.05或0.01)。與LSGB組相比，RSGB組EPINE及NPY值均升高有統(tǒng)計(jì)學(xué)意義（P＜0.05）。結(jié)論：1.星狀神經(jīng)節(jié)阻滯通過增強(qiáng)心肌的收縮和舒張功能，對(duì)SD大鼠離體心功能有促進(jìn)作用；2.左右兩側(cè)星狀神經(jīng)節(jié)對(duì)心臟的交感神經(jīng)支配區(qū)域不同，，兩側(cè)星狀神經(jīng)節(jié)阻滯對(duì)大鼠離體心功能的影響存在差異，右側(cè)星狀神經(jīng)節(jié)阻滯增強(qiáng)心功能的效果更佳。
[Abstract]:Objective: To observe the effect of stellate ganglion block on the isolated cardiac function of the isolated cardiac perfusion device in the rat of SD rat. So as to provide a theoretical basis for reducing the occurrence of cardiovascular events in the perioperative period. Method:24 to 10 weeks Age? Male SD rats weighing 180 ~ 220 g were randomly divided into 3 groups by random digital method.8 of each group: (1) left stellate ganglion block (LS group); (2) right stellate ganglion block (RS group); (3) operation control group (group C (1) After the intraperitoneal injection of the rat was completed, the left and right pectoral muscles were separated, and the stellate ganglion, the buried tube and the skin were found along the esophagus. After 7 days, the planetary ganglion block was used to observe the presence of the Horner's syndrome. Week. After the establishment of the stellate ganglion block model, the isolated cardiac perfusion model of SD rats was established by the Langendorff in-vitro cardiac perfusion device. Type: After the rats were injected with heparin, the rats were fixed in the supine position, the chest was quickly opened, the heart was removed, the aorta was isolated from the K-H solution cold at 4 鈩

本文編號(hào)：2501886