泡沫硬化劑聯(lián)合電凝閉合兔靜脈病理學(xué)研究
發(fā)布時(shí)間：2019-06-18 15:26
【摘要】：目的：本研究通過(guò)動(dòng)物實(shí)驗(yàn)，動(dòng)態(tài)觀察運(yùn)用聚桂醇泡沫硬化劑聯(lián)合電凝閉合兔靜脈病理學(xué)變化過(guò)程，運(yùn)用VEGF免疫組織化學(xué)染色，觀察實(shí)驗(yàn)兔的靜脈血管內(nèi)皮細(xì)胞的破壞、再生及血管是否再通等情況。觀察、比較運(yùn)用聚桂醇泡沫硬化劑聯(lián)合電凝對(duì)靜脈閉合效果以及靜脈血管再通的影響。為聚桂醇泡沫硬化劑及其聯(lián)合電凝用于治療下肢靜脈曲張?zhí)峁⿲?shí)驗(yàn)病理學(xué)依據(jù)。 方法：健康成年普通家兔75只，隨機(jī)分為3組，再將各組按取標(biāo)本時(shí)間再分別隨機(jī)等分5小組，每組5只家兔，做好編號(hào)。以不同方法處理實(shí)驗(yàn)家兔雙側(cè)股靜脈，A組行聚桂醇泡沫硬化劑注射，應(yīng)用1%濃度聚桂醇注射液與空氣按1：4配制泡沫硬化劑；B組行電凝術(shù)，電凝能量選擇30～40W；C組聯(lián)合運(yùn)用兩種方法，電凝后注射硬化劑。實(shí)驗(yàn)處理后將各再等分小組家兔分別1d、3d、7d、14d、3m以過(guò)量麻醉處死，取家兔股靜脈及其周圍組織作為標(biāo)本，10％甲醛溶液固定，常規(guī)石蠟包埋，行HE染色和VEGF免疫組織化學(xué)染色。光鏡下觀察：無(wú)菌性炎癥改變情況，血管壁內(nèi)皮損傷程度及周圍組織破壞、血栓形成情況，纖維化的程度，血管再通等；觀察VEGF陽(yáng)性結(jié)果為血管內(nèi)皮細(xì)胞及血管周圍細(xì)胞胞漿染成淡黃至棕黃色，每張切片隨機(jī)觀察l0個(gè)高倍視野(×400)，統(tǒng)計(jì)各組各時(shí)段VEGF陽(yáng)性細(xì)胞百分比(PP)及陽(yáng)性細(xì)胞染色強(qiáng)度（SI），計(jì)算免疫反應(yīng)積分（IRS=PP*SI）,各組各時(shí)段評(píng)分采用多樣本方差分析，q檢驗(yàn)。用SPSS19.0軟件進(jìn)行統(tǒng)計(jì)處理。 結(jié)果：術(shù)后1d，三組均出現(xiàn)血管內(nèi)皮細(xì)胞破壞，血管壁細(xì)胞水腫，血小板聚集，血栓形成；電凝組及聯(lián)合組血管周圍組織破壞嚴(yán)重，纖維細(xì)胞凝固樣壞死，平滑肌細(xì)胞結(jié)構(gòu)不清，無(wú)菌性炎癥較聚桂醇泡沫硬化劑組嚴(yán)重；VEGF陽(yáng)性細(xì)胞免疫反應(yīng)積分（IRS）示三組無(wú)明顯差異（P0.05）。術(shù)后3d，聚桂醇泡沫硬化劑組血管內(nèi)皮細(xì)胞破壞、管壁細(xì)胞水腫、無(wú)菌性炎癥反應(yīng)等達(dá)到高峰，電凝組及聯(lián)合組較術(shù)后1d無(wú)明顯變化；三組VEGF陽(yáng)性細(xì)胞IRS均低，且P0.05，差別無(wú)統(tǒng)計(jì)學(xué)意義。術(shù)后7d，三組均有部分血栓機(jī)化與管壁黏連，血管壁破壞加重，炎癥細(xì)胞浸潤(rùn)；三組VEGF陽(yáng)性細(xì)胞IRS增高，且P0.05，差別無(wú)統(tǒng)計(jì)學(xué)意義。術(shù)后14d，，，三組均有血栓機(jī)化，血管壁嚴(yán)重破壞、輪廓形態(tài)不清或消失，炎癥反應(yīng)減輕；三組VEGF陽(yáng)性細(xì)胞IRS繼續(xù)增高，且P0.05，差別無(wú)統(tǒng)計(jì)學(xué)意義。術(shù)后3m，三組均出現(xiàn)閉塞靜脈血管再通，周圍壞死組織吸收，炎癥消失。 結(jié)論：1、聚桂醇泡沫硬化劑、電凝或兩者聯(lián)合均可有效閉塞家兔靜脈。 2、聚桂醇泡沫硬化劑、電凝或兩者聯(lián)合使用閉塞家兔靜脈都存在血管內(nèi)皮細(xì)胞再生現(xiàn)象，遠(yuǎn)期閉塞靜脈再通。 3、聚桂醇泡沫硬化劑聯(lián)合電凝較單獨(dú)運(yùn)用兩種方法閉塞靜脈后不增加也不減少內(nèi)皮細(xì)胞增生及血管再通的程度。
[Abstract]:Objective: To observe the changes of venous pathology in the rabbit by using the combination of polycinnamyl alcohol and foam sclerosing agent, and to observe the damage, regeneration and the vascular recanalization of the vascular endothelial cells in the experimental rabbits by using the immunohistochemical staining of VEGF. The effect of combined electrocoagulation on the effect of combined electrocoagulation on the effect of vein closure and the recanalization of venous blood vessels was compared. The invention provides experimental and pathological basis for the treatment of varicose veins of the lower limbs by using the polycinnamyl alcohol foam hardener and the combined electrocoagulation thereof. Methods:75 healthy adult rabbits were randomly divided into 3 groups. The two-sided femoral vein of the experimental rabbits was treated with different methods. The group A was injected with the foam hardener of the group A. The foam hardening agent was prepared by 1:4 with the concentration of 1%. The electrocoagulation of group B and the selection of electrocoagulation energy were 30-40W, and the combination of group C and group C was used in combination. Method of injection hardening after electrocoagulation The rabbit femoral vein and its surrounding tissues were used as the specimen, the 10% formaldehyde solution was fixed, the normal paraffin was embedded, the HE staining and the immunohistochemical staining of VEGF were performed. Color. Under light microscope, the condition of aseptic inflammation, the degree of vascular wall injury, the destruction of surrounding tissues, the formation of thrombus, the degree of fibrosis, and the recanalization of the blood vessel were observed. The positive results of VEGF were observed as vascular endothelial cells and the cytoplasm of the peripheral cells of the blood vessel were stained with pale yellow to brown yellow. The percentage of VEGF-positive cells (PP) and positive-cell staining intensity (SI) in each group of each group were counted randomly, and the percentage of VEGF-positive cells (PP) and positive-cell staining intensity (SI) in each group were counted, and the immune response integral (IRS = PP * SI) was calculated. Inspection. Statistical Office with SPSS19.0 software Results: In the first day of the operation, vascular endothelial cell destruction, vascular wall cell edema, platelet aggregation and thrombosis were observed in three groups. The results showed that there was no significant difference between the three groups (P0.05). 05).3 d after the operation, the vascular endothelial cells of the polycyl foam hardener group were damaged, the wall cell edema and the aseptic inflammation reaction reached the peak, and there was no significant change in the electrocoagulation group and the joint group after the operation. The expression of VEGF positive cells in the three groups was higher than that of the other three groups, and there was no statistical difference between the three groups. The expression of VEGF-positive cells in three groups was not clear or disappeared, and the inflammatory response was relieved. The IRS of three groups of VEGF-positive cells continued to increase, and the difference of the three groups was not statistically significant. The results showed that 3 m after operation, the three groups of the three groups showed a recanalization of the vein, the surrounding necrotic tissue was absorbed, and the inflammation Conclusion:1, Cassia foam hardening agent, electrocoagulation, or both can be effectively closed. The blood vessel endothelial cell regeneration was found in the vein of the rabbit, the foam hardening agent, the electrocoagulation, or the combination of both. 3. The combined electrocoagulation of the polycinnamyl alcohol foam sclerosing agent and the electrocoagulation alone did not increase the increase of the endothelial cell by two methods.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R654.4
本文編號(hào)：2501592
[Abstract]:Objective: To observe the changes of venous pathology in the rabbit by using the combination of polycinnamyl alcohol and foam sclerosing agent, and to observe the damage, regeneration and the vascular recanalization of the vascular endothelial cells in the experimental rabbits by using the immunohistochemical staining of VEGF. The effect of combined electrocoagulation on the effect of combined electrocoagulation on the effect of vein closure and the recanalization of venous blood vessels was compared. The invention provides experimental and pathological basis for the treatment of varicose veins of the lower limbs by using the polycinnamyl alcohol foam hardener and the combined electrocoagulation thereof. Methods:75 healthy adult rabbits were randomly divided into 3 groups. The two-sided femoral vein of the experimental rabbits was treated with different methods. The group A was injected with the foam hardener of the group A. The foam hardening agent was prepared by 1:4 with the concentration of 1%. The electrocoagulation of group B and the selection of electrocoagulation energy were 30-40W, and the combination of group C and group C was used in combination. Method of injection hardening after electrocoagulation The rabbit femoral vein and its surrounding tissues were used as the specimen, the 10% formaldehyde solution was fixed, the normal paraffin was embedded, the HE staining and the immunohistochemical staining of VEGF were performed. Color. Under light microscope, the condition of aseptic inflammation, the degree of vascular wall injury, the destruction of surrounding tissues, the formation of thrombus, the degree of fibrosis, and the recanalization of the blood vessel were observed. The positive results of VEGF were observed as vascular endothelial cells and the cytoplasm of the peripheral cells of the blood vessel were stained with pale yellow to brown yellow. The percentage of VEGF-positive cells (PP) and positive-cell staining intensity (SI) in each group of each group were counted randomly, and the percentage of VEGF-positive cells (PP) and positive-cell staining intensity (SI) in each group were counted, and the immune response integral (IRS = PP * SI) was calculated. Inspection. Statistical Office with SPSS19.0 software Results: In the first day of the operation, vascular endothelial cell destruction, vascular wall cell edema, platelet aggregation and thrombosis were observed in three groups. The results showed that there was no significant difference between the three groups (P0.05). 05).3 d after the operation, the vascular endothelial cells of the polycyl foam hardener group were damaged, the wall cell edema and the aseptic inflammation reaction reached the peak, and there was no significant change in the electrocoagulation group and the joint group after the operation. The expression of VEGF positive cells in the three groups was higher than that of the other three groups, and there was no statistical difference between the three groups. The expression of VEGF-positive cells in three groups was not clear or disappeared, and the inflammatory response was relieved. The IRS of three groups of VEGF-positive cells continued to increase, and the difference of the three groups was not statistically significant. The results showed that 3 m after operation, the three groups of the three groups showed a recanalization of the vein, the surrounding necrotic tissue was absorbed, and the inflammation Conclusion:1, Cassia foam hardening agent, electrocoagulation, or both can be effectively closed. The blood vessel endothelial cell regeneration was found in the vein of the rabbit, the foam hardening agent, the electrocoagulation, or the combination of both. 3. The combined electrocoagulation of the polycinnamyl alcohol foam sclerosing agent and the electrocoagulation alone did not increase the increase of the endothelial cell by two methods.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R654.4
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 董國(guó)祥;電凝法治療下肢靜脈曲張[J];北京醫(yī)科大學(xué)學(xué)報(bào);2000年02期
2 張德守,郭廣銘,郅樹升,徐健;電凝法微創(chuàng)治療下肢靜脈曲張[J];臨床外科雜志;2003年04期
3 吳丹明,王成剛,易巍;介入導(dǎo)管電凝術(shù)治療下肢靜脈曲張的臨床研究[J];中國(guó)臨床醫(yī)學(xué)影像雜志;2004年08期
4 王銀中;韓朝陽(yáng);;電凝加股段大隱靜脈剝脫治療下肢靜脈曲張76例[J];中國(guó)現(xiàn)代普通外科進(jìn)展;2010年11期
5 王永光;;聚桂醇:新型的微創(chuàng)硬化治療技術(shù)[J];微創(chuàng)醫(yī)學(xué);2011年01期
6 張立偉;郭修海;黃智勇;金輝;;泡沫硬化療法治療靜脈疾病的并發(fā)癥和不良反應(yīng)[J];醫(yī)學(xué)綜述;2011年01期
7 符偉國(guó),徐欣,王玉琦,郭大喬,陳斌,楊玨,蔣俊豪;微創(chuàng)刨吸術(shù)治療靜脈曲張22例報(bào)告[J];中國(guó)實(shí)用外科雜志;2003年01期
8 劉小平;溫朝陽(yáng);郭偉;梁發(fā)啟;程志剛;唐杰;;泡沫硬化療法治療大隱靜脈曲張34例報(bào)告[J];中國(guó)實(shí)用外科雜志;2006年02期
9 王成剛;吳丹明;周玉斌;易巍;賈琪;孫雨莘;;電凝術(shù)治療下肢靜脈曲張的評(píng)價(jià)[J];中國(guó)實(shí)用外科雜志;2006年06期
10 單平;李鳴;金煒;張鴻坤;趙海格;陳旭東;田路;潘松齡;;射頻閉合與傳統(tǒng)剝脫術(shù)治療下肢靜脈曲張的對(duì)照研究[J];中華普通外科雜志;2006年02期
本文編號(hào)：2501592
本文鏈接：http://sikaile.net/yixuelunwen/mazuiyixuelunwen/2501592.html
最近更新
教材專著
