【摘要】：目的比較霍山石斛(DHP)、鐵皮石斛(DOP)、流蘇石斛(DFP)、鼓槌石斛(DCP)、金釵石斛(DNP)和細莖石斛(DMP)等6種石斛多糖對亞急性酒精性肝損傷的保護作用并探討其可能機制。方法將實驗小鼠隨機分為正常對照組、模型對照組、陽性對照組(枸杞多糖200 mg·kg~(-1))和6種石斛多糖高(200 mg·kg~(-1))、中(100 mg·kg~(-1))、低(50 mg·kg~(-1))3個劑量處理組。其中,陽性對照組和石斛處理組分別經(jīng)口灌胃多糖樣品30 d,正常對照組和模型對照組給予等體積蒸餾水。從第16天起,模型對照組、陽性對照組和石斛處理組每天經(jīng)口灌胃30%乙醇(V/V,10 m L·kg~(-1)),建立亞急性酒精性肝損傷模型。末次給藥后,禁食4 h,麻醉、采血、取肝組織,檢測血清谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、堿性磷酸酶(ALP)、總膽紅素(TBIL)、總膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白膽固醇(LDL-C)和高密度脂蛋白膽固醇(HDL-C)的含量,以及肝臟總膽固醇、甘油三酯、乙醇脫氫酶(ADH)、乙醛脫氫酶(ALDH)、抗氧化酶(SOD、CAT、GSH-PX、GR、GST)、還原型谷胱甘肽(GSH)和丙二醛(MDA)等指標,觀察小鼠肝損傷組織病理學變化,并用RT-PCR檢測CYP2E1、TNF-α和IL-1的mRNA表達水平。結(jié)果 6種石斛多糖對亞急性酒精性肝損傷均有一定程度的保護作用。其中,霍山石斛和鐵皮石斛可有效抑制因酒精升高的血清谷丙轉(zhuǎn)氨酶、谷草轉(zhuǎn)氨酶、堿性磷酸酶、總膽紅素、總膽固醇、甘油三酯、低密度脂蛋白膽固醇及肝組織總膽固醇、甘油三酯和丙二醛的水平,提升因酒精降低的血清低密度脂蛋白膽固醇及肝組織SOD、CAT、GSH-PX、GR、GST和GSH的水平,上調(diào)肝組織乙醇脫氫酶和乙醛脫氫酶活性,下調(diào)肝組織CYP2E1、TNF-α和IL-1β的表達,減輕酒精對肝臟的病理損傷。結(jié)論石斛多糖可能是通過抑制肝組織氧化損傷和炎性損傷的途徑達到抗亞急性酒精性肝損傷的作用。6種石斛多糖抗酒精性肝損傷的活性各有不同,其中以霍山石斛和鐵皮石斛最為有效,這可能與不同石斛多糖的相對分子質(zhì)量、單糖組成和糖苷鍵鏈接方式等化學結(jié)構(gòu)不同有關(guān)。
[Abstract]:Objective to compare the (DFP), drumstick (DCP), of Dendrobium huoshan (DHP), Dendrobium candidum (DOP), with that of Dendrobium candidum (DFP),. Protective effects of six kinds of Dendrobium polysaccharides, Dendrobium nobile (DNP) and (DMP), on subacute alcoholic liver injury and their possible mechanisms. Methods the experimental mice were randomly divided into normal control group, model control group, positive control group (Lycium barbarum polysaccharide 200 mg kg~ (- 1) and six kinds of Dendrobium polysaccharide high in 200 mg kg~ (- 1),) (100 mg kg~ (- 1),). Low (50 mg kg~ (- 1) three dose treatment groups. Among them, the positive control group and the Dendrobium treatment group were treated with polysaccharide samples by oral administration for 30 days, and the normal control group and the model control group were given the same volume distilled water. From the 16th day, the model control group, positive control group and Dendrobium treatment group were treated with 30% ethanol (V 鈮，

本文編號：2486918