【摘要】：目的探討不同濃度的依托咪酯是否具有直接誘導(dǎo)人肝癌HepG2細(xì)胞凋亡的作用,尋找依托咪酯臨床治療的新途徑。方法人肝癌細(xì)胞株HepG2體外擴(kuò)增培養(yǎng)備用,選取不同濃度的依托咪酯E1、E2、E3和對照組(空白),分別培養(yǎng)12h、24h、48h后觀察。結(jié)果與空白對照組相比,培養(yǎng)12h、24h、48h后E1組、E2組人肝癌細(xì)胞株HepG2細(xì)胞凋亡率無明顯差異(P0.05),E3組凋亡率不斷增加,具有顯著性差異(P0.05);隨著培養(yǎng)時間的延長及濃度的不斷增加,E1與E2組對比人肝癌細(xì)胞株HepG2細(xì)胞凋亡率無明顯差異(t=1.732,P0.05),E1與E3組對比人肝癌細(xì)胞株HepG2細(xì)胞凋亡率具有顯著性差異(t=1.864,P0.05),E2與E3組對比人肝癌細(xì)胞株HepG2細(xì)胞凋亡率具有明顯差異(t=1.691,P0.05)。結(jié)論依托咪酯在臨床安全有效的濃度下不直接誘導(dǎo)人肝癌HepG2細(xì)胞的體外凋亡,濃度及時間達(dá)到一定峰值時可具有直接誘導(dǎo)人肝癌HepG2細(xì)胞體外凋亡的作用。
[Abstract]:Objective to investigate whether different concentrations of etomidate can directly induce apoptosis of human hepatocellular carcinoma HepG2 cells and to find a new way of clinical treatment of etomidate. Methods Human liver cancer cell line HepG2 was expanded and cultured in vitro. Different concentrations of etomidate E1, E2, E3 and control group (blank) were cultured for 12 hours, 24 hours and 48 hours respectively. Results compared with the blank control group, there was no significant difference in the apoptosis rate of human hepatocellular carcinoma cell line HepG2 in E1 group and E2 group 48 hours after culture for 12 hours, but the apoptosis rate in E3 group increased significantly (P 0.05). With the prolongation of culture time and the increase of concentration, there was no significant difference in apoptosis rate between E1 group and E2 group (t 鈮，

本文編號：2484629