【摘要】：目的：探討促紅細(xì)胞生成素(EPO)對SD大鼠坐骨神經(jīng)損傷后相應(yīng)節(jié)段脊髓組織內(nèi)Caspase-3和GAP-43表達(dá)情況及神經(jīng)修復(fù)的影響，為臨床治療周圍神經(jīng)損傷提供實(shí)驗(yàn)性依據(jù)。 方法：生長發(fā)育正常的潔凈SD大鼠45只，雌雄不限，按照隨機(jī)原則分為3個(gè)組，即對照組、模型組和實(shí)驗(yàn)組，每組15只。坐骨神經(jīng)損傷模型制作方法：大鼠麻醉完全后取俯臥位固定，手術(shù)區(qū)域常規(guī)消毒、覆蓋無菌單，在右側(cè)大腿中部梨狀肌下充分暴露坐骨神經(jīng)后用普通外科持針器壓榨坐骨神經(jīng)2次，每次30秒鐘，間隔10秒鐘，損傷長度約為2mm。對照組正常飼養(yǎng)，模型組每日腹腔注射生理鹽水，實(shí)驗(yàn)組每日腹腔注射促紅細(xì)胞生成素。于術(shù)后的1w、2w、3w每組隨機(jī)提取5只大鼠行坐骨神經(jīng)功能指數(shù)檢測，受損坐骨神經(jīng)組織學(xué)觀察，PCR方法檢測相應(yīng)節(jié)段脊髓組織內(nèi)Caspase-3和GAP-43的表達(dá)情況。 結(jié)果：坐骨神經(jīng)功能指數(shù)檢測顯示對照組各時(shí)間段正常，，模型組與實(shí)驗(yàn)組顯著降低，但第1w無明顯差異（P〉0.05），第2w、3w實(shí)驗(yàn)組神經(jīng)功能恢復(fù)明顯優(yōu)于模型組（P〈0.05）。HE染色顯示對照組坐骨神經(jīng)組織結(jié)構(gòu)基本正常，神經(jīng)纖維排列整齊、染色較均勻，組織未見明顯水腫和炎細(xì)胞浸潤；模型組的組織切片顯示軸突腫脹明顯、空泡變性，細(xì)胞核固縮，神經(jīng)組織腫脹，炎細(xì)胞浸潤明顯；實(shí)驗(yàn)組同期病理表現(xiàn)較模型組明顯減輕。相應(yīng)節(jié)段的脊髓組織Caspase-3和GAP-43在對照組呈低表達(dá)狀態(tài)，模型組和實(shí)驗(yàn)組在神經(jīng)損傷后表達(dá)均增加，第1w兩組表達(dá)情況差異較輕微，第2w、3w實(shí)驗(yàn)組Caspase-3表達(dá)較模型組明顯減少，GAP-43實(shí)驗(yàn)組較模型組表達(dá)明顯增加。 結(jié)論：EPO可能通過抑制受損神經(jīng)組織相應(yīng)節(jié)段脊髓內(nèi)Caspase-3的表達(dá)，促進(jìn)GAP-43的表達(dá)，對SD大鼠坐骨神經(jīng)損傷發(fā)揮保護(hù)、修復(fù)作用。
[Abstract]:Aim: to investigate the effect of erythropoietin (EPO) (EPO) on the expression of Caspase-3 and GAP-43 in the spinal cord of SD rats after sciatic nerve injury, and the effect of erythropoietin on nerve repair in order to provide experimental evidence for clinical treatment of peripheral nerve injury. Methods: 45 clean SD rats with normal growth and development were randomly divided into three groups: control group, model group and experimental group, with 15 rats in each group. The model of sciatic nerve injury was made: the rats were anesthetized completely and fixed in prone position, the operation area was routinely sterilized, and the sterile single was covered. After fully exposing the sciatic nerve under the piriform muscle in the middle part of the right thigh, the sciatic nerve was squeezed twice with a general surgical needle holder for 30 seconds at an interval of 10 seconds, and the length of the injury was about 2 mm. The model group was intraperitoneally injected with saline, and the experimental group was intraperitoneally injected with erythropoietin (Erythropoietin). At 1 week, 2 weeks and 3 weeks after operation, 5 rats in each group were randomly extracted for sciatic nerve function index detection, histological observation of injured sciatic nerve, and PCR method was used to detect the expression of Caspase-3 and GAP-43 in the corresponding spinal cord tissue. Results: the sciatic nerve function index showed that the control group was normal at each period of time, the model group and the experimental group decreased significantly, but there was no significant difference at the 1st week (P > 0.05), at the 2nd week, The recovery of nerve function in the experimental group was significantly better than that in the model group (P < 0. 05). HE staining showed that the sciatic nerve in the control group was basically normal, the nerve fibers were arranged neatly, the staining was more uniform, and there was no obvious edema and infiltration of inflammatory cells in the tissue. The histological sections of the model group showed obvious axonal swelling, vacuolar degeneration, nuclear shrinkage, nerve tissue swelling and inflammatory cell infiltration, and the pathological manifestations of the experimental group were significantly less than those of the model group at the same time. The expression of Caspase-3 and GAP-43 in the spinal cord tissue of the corresponding segments was low in the control group, and increased in both the model group and the experimental group after nerve injury. The difference between the two groups at the 1st week was slight, and the expression at the 2nd week was slightly different between the two groups. After 3 weeks, the expression of Caspase-3 in experimental group was significantly lower than that in model group, and the expression in GAP-43 group was significantly higher than that in model group. Conclusion: EPO may protect and repair sciatic nerve injury in SD rats by inhibiting the expression of Caspase-3 in the corresponding segments of the injured nerve tissue and promoting the expression of GAP-43.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R651.3

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相關(guān)期刊論文 前3條

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