【摘要】：榜那為常用藏藥,民間及臨床的應(yīng)用均取其消炎、鎮(zhèn)痛及麻醉等功效,主要用于腰肌勞損,風(fēng)濕關(guān)節(jié)痛等疾病的治療。榜那藥材為毛茛科烏頭屬植物,含有多種生物堿,其中雙酯型生物堿是一類毒性較大的成分。由于榜那藥材中所含生物堿既是活性成分又是其毒性成分,所以建立完善的榜那質(zhì)量標準對保證其臨床用藥安全有效有重要意義。 《中國藥典》(2010年版一部)未收載榜那藥材的質(zhì)量標準,在《衛(wèi)生部藥品標準·藏藥(第一冊)》(1995年版)中也只有部分簡單的規(guī)定,除有[性狀]、[鑒別]、[性味]、[功能與主治]、[用法與用量]和[貯藏]等項外,缺乏作為現(xiàn)代藥品質(zhì)量標準標志的活性或毒性成分的[含量]及[檢查]項等的規(guī)定,標準極不完善,為了有效的控制藥物的安全性及有效性,需要建立一個完善的質(zhì)量標準。 本研究參照《中國藥典》2010版一部,對其水分、灰分和浸出物進行了測定,并暫定其限度分別為12.5%、7%和15%；采用高效液相色譜法,建立了烏頭堿的限量測定法,該方法專屬性強,耐用性好,精密度高,平均回收率為99.15%,RSD=0.7%；采用紫外-可見分光光度法測定,建立了總生物堿的含量測定方法,本法測定的重復(fù)性良好,RSD=2.54%,平均加樣回收率為95.41%,RSD=0.43%。另外,對榜那藥材的薄層鑒別也進行了研究,但研究結(jié)果顯示,所有藥材找不到共有斑點,故無法建立薄層鑒別。
[Abstract]:Pangna is a commonly used Tibetan medicine, folk and clinical use of its anti-inflammatory, analgesic and anesthetic efficacy, mainly used in the treatment of lumbar muscle strain, rheumatic arthralgia and other diseases. Pongna is a kind of aconitum plant of Ranunculaceae, which contains many alkaloids, among which diester alkaloid is a kind of toxic component. Since the alkaloids contained in Pangna are both active and toxic, it is of great significance to establish a perfect quality standard for the safety and effectiveness of their clinical use. The Chinese Pharmacopoeia (2010 edition No. 1) does not contain the quality standard of that medicine. In the Drug Standard Tibetan Medicine of the Ministry of Health (Volume 1) (1995 edition), there are only some simple provisions, except for [characters], [identification]. With the exception of [sexual taste], [function and main treatment], [usage and dosage] and [storage], there is a lack of [content] and [examination] items, which are used as markers of quality standards for modern medicines, and so on, and the standards are extremely imperfect, In order to effectively control the safety and effectiveness of drugs, it is necessary to establish a perfect quality standard. According to the first part of the 2010 edition of Chinese Pharmacopoeia, the moisture, ash and extract were determined, and the tentative limits were 12.5%, 7% and 15%, respectively. The limit determination of aconitine was established by high performance liquid chromatography (HPLC). The method has high specificity, good durability and high precision. The average recovery is 99.15% and RSD is 0.7%. A method for the determination of total alkaloids by UV-vis spectrophotometry was established. The repeatability of the method was good. The average recovery of RSD=2.54%, and RSD were 95.41% and 0.43%, respectively. In addition, the TLC identification of Pangna was also studied, but the results showed that no common spots could be found in all medicinal materials, so the TLC identification could not be established.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R29

【參考文獻】

相關(guān)期刊論文 前10條

1 陳鳳娥;;鐵棒錘的研究進展[J];安徽農(nóng)業(yè)科學(xué);2007年32期

2 劉世巍;劉立紅;楊敏麗;張廣宏;梁永鋒;;正交設(shè)計法優(yōu)化伏毛鐵棒錘根中總生物堿的提取工藝[J];安徽農(nóng)業(yè)科學(xué);2008年21期

3 劉立紅;梁永鋒;劉世巍;丁建海;;微波提取伏毛鐵棒錘總生物堿工藝的優(yōu)化[J];安徽農(nóng)業(yè)科學(xué);2010年36期

4 顧潔;王俊;王鴻;彭勵;;伏毛鐵棒錘不同器官生物堿含量動態(tài)變化研究[J];西北植物學(xué)報;2010年01期

5 林麗;魏學(xué)明;馬偉麗;李碩;杜_";;甘肅栽培鐵棒錘與野生鐵棒錘生藥學(xué)研究[J];甘肅中醫(yī)學(xué)院學(xué)報;2009年05期

6 胡君茹;姜華;;藏藥鐵棒錘的化學(xué)成分及藥理作用研究進展[J];甘肅中醫(yī);2006年11期

7 周友紅;呼海濤;;淺述烏頭堿類藥物的功效及毒性[J];河南中醫(yī);2008年07期

8 劉智勇,阿萍,劉振東,劉兵,王曙;榜那不同基源品種的鑒別和含量測定[J];華西藥學(xué)雜志;2003年05期

9 黃學(xué)娣;王曙;嚴曉梁;;HPLC-ELSD測定工布烏頭中的塔拉薩敏[J];華西藥學(xué)雜志;2008年02期

10 曾洪學(xué);王俊;張守宗;屈興紅;關(guān)雅靜;;伏毛鐵棒錘研究概況[J];江蘇中醫(yī)藥;2007年06期

，

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