采用高胰島素—正葡萄糖鉗夾技術(shù)觀察耳針對家兔胰島素敏感性的影響
發(fā)布時間：2019-01-01 16:16
【摘要】：研究目的： 采用高胰島素-正葡萄糖鉗夾技術(shù)評價耳針對家兔血糖的干預作用,從而確定耳針是提高胰島素敏感性的一種方法,并闡明其干預作用的機制；為耳針治療2型糖尿病(T2DM)提供實驗依據(jù),為臨床推廣應用打下基礎。 實驗方法： 1.選取健康新西蘭家兔12只,隨機分為清醒組和麻醉組,每組6只(n=6)。清醒組：在非麻醉狀態(tài)下,行家兔雙側(cè)耳緣靜脈穿刺術(shù)。麻醉組：在麻醉狀態(tài)下行家兔頸動靜脈插管術(shù)。兩組家兔分別進行高胰島素-正葡萄糖鉗夾實驗,根據(jù)實驗周期、血糖波動等因素對兩種方法進行比較。 2.選取健康新西蘭家兔16只,隨機分為對照組和耳針組,每組8只(n=8)。耳針組：模擬人體取穴,在相當于人體耳穴胰點的家兔耳甲艇處,雙側(cè),電針治療,疏密波,40min。對照組：除未電針外,與耳針組相同。兩組家兔分別采用高胰島素-正葡萄糖鉗夾技術(shù)檢測葡萄糖輸注率(GIR),并測定家兔空腹血糖(FBG)、鉗夾實驗期間血糖及穩(wěn)態(tài)血糖,對家兔的胰島素敏感性進行評估。實驗結(jié)果： 1.非麻醉狀態(tài)下的家兔雙側(cè)耳緣靜脈穿刺的高胰島素-正葡萄糖鉗夾實驗中家兔血糖在正常范圍內(nèi)波動,操作過程簡單,成功率高；而麻醉狀態(tài)下的家兔頸動靜脈插管高胰島素-正葡萄糖鉗夾實驗中：家兔血糖波動較大,且不在正常范圍內(nèi),操作過程復雜,成功率低。 2.非麻醉狀態(tài)下的家兔雙側(cè)耳緣靜脈穿刺的高胰島素-正葡萄糖鉗夾實驗中對照組和耳針組的GIR變異系數(shù)(CVGIR)分別為1.28%、0.21%,耳針組明顯低于對照組(P0.05)。同時對照組、耳針組的空腹血糖與穩(wěn)態(tài)血糖比較后差異無統(tǒng)計學意義(P0.05)。 3.非麻醉狀態(tài)下的家兔雙側(cè)耳緣靜脈穿刺的高胰島素-正葡萄糖鉗夾實驗中：耳針組家兔的GIR明顯升高,與對照組比較差異顯著(P0.05)。耳針組與對照組家兔的GIR在各個時間點的比較：輸糖后40min、50min及60min時,耳針組GIR升高,差異顯著(P0.05)。 實驗結(jié)論： 1.在非麻醉狀態(tài)下,采用家兔雙側(cè)耳緣靜脈穿刺的高胰島素-正葡萄糖鉗夾實驗,具有一定的可行性和可靠性,穩(wěn)定性高、操作簡便、死亡率低、重復性好。即在經(jīng)典方法的基礎上,成功建立家兔高胰島素-正葡萄糖鉗夾模型,突破了國內(nèi)基礎研究中只在小動物(大鼠及小鼠)應用的限制。 2.實驗過程中家兔血糖逐漸趨于穩(wěn)定,鉗夾實驗的穩(wěn)定性較好。而耳針組鉗夾實驗結(jié)果具有更高的穩(wěn)定性。 3.在輸入葡萄糖后的40min、50min、60min時耳針組與對照組有明顯差異,提示耳針可有效提高機體的胰島素敏感性,增加外周組織對糖的利用率。它可能是通過改善肝臟和肌肉脂肪等外周組織對胰島素的敏感性,提高周圍組織對葡萄糖的利用率這一途徑來調(diào)節(jié)機體的糖代謝,最終達到降低血糖的效果。
[Abstract]:Objective: to evaluate the effect of hyperinsulin-glucose clamp on blood glucose in rabbits, and to determine that ear acupuncture is a method to increase insulin sensitivity and clarify the mechanism of intervention. To provide experimental basis for auricular acupuncture treatment of type 2 diabetes mellitus (T2DM) and lay a foundation for clinical application. Experimental method: 1. Twelve healthy New Zealand rabbits were randomly divided into awake group and anesthetic group with 6 rabbits in each group. Lucid group: under the condition of non-anaesthesia, bilateral auricular vein puncture was performed in rabbits. Anesthesia group: cervical arteriovenous intubation was performed in anesthetized rabbits. The hyperinsulin-glucose clamp test was carried out in two groups of rabbits, and the two methods were compared according to the experimental period and the fluctuation of blood glucose. 2. Sixteen healthy New Zealand rabbits were randomly divided into control group and ear acupuncture group (n = 8). Auricular acupuncture group: simulated human body to take points, at the rabbit ear nail boat equivalent to the human ear point pancreatic point, bilateral, electroacupuncture treatment, dredge wave, 40 minutes. The control group was the same as the ear acupuncture group except for no electroacupuncture. Hyperinsulin-positive glucose clamp technique was used to detect glucose infusion rate (GIR),) and fasting blood glucose (FBG),) clamp in two groups. The insulin sensitivity of rabbits was evaluated. Experimental results: 1. In the hyperinsulin-positive glucose clamp experiment of bilateral auricular vein puncture in non-anesthetized rabbits, the blood glucose fluctuated within the normal range, the operation process was simple and the success rate was high. However, in the hyperinsulin-positive glucose clamp experiment of rabbit cervical arteriovenous intubation under anesthesia, the blood glucose fluctuated greatly and was not within the normal range, the operation process was complicated and the success rate was low. 2. In the hyperinsulin-positive glucose clamp test of bilateral auricular vein puncture in non-anesthetized rabbits, the (CVGIR) of GIR in the control group and the ear acupuncture group was 1.28 and 0.21, respectively, and that in the ear acupuncture group was significantly lower than that in the control group (P0.05). At the same time, there was no significant difference in fasting blood glucose and steady-state blood glucose between the control group and the ear acupuncture group (P0.05). 3. In the hyperinsulin-positive glucose clamp experiment of bilateral auricular margin vein puncture in non-anesthetized rabbits, the GIR of the auricular acupuncture group was significantly higher than that of the control group (P0.05). The comparison of GIR between the auricular acupuncture group and the control group at each time point: the GIR of the auricular acupuncture group was significantly higher than that of the control group (P0.05). Experimental conclusions: 1. Under the condition of non-anaesthesia, the high insulin-glucose clamp test with double lateral auricular vein puncture in rabbits is feasible and reliable, with high stability, simple operation, low mortality and good reproducibility. On the basis of the classical method, the rabbit hyperinsulin-glucose clamp model was successfully established, which broke through the limitation of application in domestic basic research only in small animals (rats and mice). 2. During the experiment, the blood sugar of rabbits tended to be stable, and the stability of clamp experiment was better. The results of the pinching experiment in the ear acupuncture group were more stable than those in the ear acupuncture group. 3. There was a significant difference between the auricular acupuncture group and the control group at 40 min, 50 min and 60 min after glucose infusion, which suggested that ear acupuncture could effectively improve the insulin sensitivity of the body and increase the utilization rate of glucose in peripheral tissues. It may regulate glucose metabolism by improving the sensitivity of peripheral tissues such as liver and muscle fat to insulin and increasing the utilization rate of glucose in peripheral tissues.
【學位授予單位】：安徽中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R245
[Abstract]:Objective: to evaluate the effect of hyperinsulin-glucose clamp on blood glucose in rabbits, and to determine that ear acupuncture is a method to increase insulin sensitivity and clarify the mechanism of intervention. To provide experimental basis for auricular acupuncture treatment of type 2 diabetes mellitus (T2DM) and lay a foundation for clinical application. Experimental method: 1. Twelve healthy New Zealand rabbits were randomly divided into awake group and anesthetic group with 6 rabbits in each group. Lucid group: under the condition of non-anaesthesia, bilateral auricular vein puncture was performed in rabbits. Anesthesia group: cervical arteriovenous intubation was performed in anesthetized rabbits. The hyperinsulin-glucose clamp test was carried out in two groups of rabbits, and the two methods were compared according to the experimental period and the fluctuation of blood glucose. 2. Sixteen healthy New Zealand rabbits were randomly divided into control group and ear acupuncture group (n = 8). Auricular acupuncture group: simulated human body to take points, at the rabbit ear nail boat equivalent to the human ear point pancreatic point, bilateral, electroacupuncture treatment, dredge wave, 40 minutes. The control group was the same as the ear acupuncture group except for no electroacupuncture. Hyperinsulin-positive glucose clamp technique was used to detect glucose infusion rate (GIR),) and fasting blood glucose (FBG),) clamp in two groups. The insulin sensitivity of rabbits was evaluated. Experimental results: 1. In the hyperinsulin-positive glucose clamp experiment of bilateral auricular vein puncture in non-anesthetized rabbits, the blood glucose fluctuated within the normal range, the operation process was simple and the success rate was high. However, in the hyperinsulin-positive glucose clamp experiment of rabbit cervical arteriovenous intubation under anesthesia, the blood glucose fluctuated greatly and was not within the normal range, the operation process was complicated and the success rate was low. 2. In the hyperinsulin-positive glucose clamp test of bilateral auricular vein puncture in non-anesthetized rabbits, the (CVGIR) of GIR in the control group and the ear acupuncture group was 1.28 and 0.21, respectively, and that in the ear acupuncture group was significantly lower than that in the control group (P0.05). At the same time, there was no significant difference in fasting blood glucose and steady-state blood glucose between the control group and the ear acupuncture group (P0.05). 3. In the hyperinsulin-positive glucose clamp experiment of bilateral auricular margin vein puncture in non-anesthetized rabbits, the GIR of the auricular acupuncture group was significantly higher than that of the control group (P0.05). The comparison of GIR between the auricular acupuncture group and the control group at each time point: the GIR of the auricular acupuncture group was significantly higher than that of the control group (P0.05). Experimental conclusions: 1. Under the condition of non-anaesthesia, the high insulin-glucose clamp test with double lateral auricular vein puncture in rabbits is feasible and reliable, with high stability, simple operation, low mortality and good reproducibility. On the basis of the classical method, the rabbit hyperinsulin-glucose clamp model was successfully established, which broke through the limitation of application in domestic basic research only in small animals (rats and mice). 2. During the experiment, the blood sugar of rabbits tended to be stable, and the stability of clamp experiment was better. The results of the pinching experiment in the ear acupuncture group were more stable than those in the ear acupuncture group. 3. There was a significant difference between the auricular acupuncture group and the control group at 40 min, 50 min and 60 min after glucose infusion, which suggested that ear acupuncture could effectively improve the insulin sensitivity of the body and increase the utilization rate of glucose in peripheral tissues. It may regulate glucose metabolism by improving the sensitivity of peripheral tissues such as liver and muscle fat to insulin and increasing the utilization rate of glucose in peripheral tissues.
【學位授予單位】：安徽中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R245
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相關(guān)期刊論文 前10條
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