丹龍醒腦方對(duì)腦缺血再灌注大鼠血管新生的影響及機(jī)理探討
發(fā)布時(shí)間：2018-10-23 18:40
【摘要】：目的：通過(guò)動(dòng)物實(shí)驗(yàn),觀察丹龍醒腦方對(duì)腦缺血再灌注損傷大鼠大腦血管新生及其因子的影響,探討其對(duì)缺血性中風(fēng)病理機(jī)制的干預(yù)作用。 方法：本實(shí)驗(yàn)采用MCAO法建立SD雄性大鼠腦缺血再灌注損傷模型,將造模成功的存活大鼠按隨機(jī)數(shù)字表法隨機(jī)分成6組：丹龍醒腦方大劑量組、丹龍醒腦方中劑量組、丹龍醒腦方小劑量組、尼莫地平陽(yáng)性對(duì)照組、模型組、假手術(shù)組,每組8只,除假手術(shù)組外,其余各組均采用MCAO法造模,術(shù)后2h恢復(fù)再灌注,假手術(shù)組只結(jié)扎頸外動(dòng)脈,不進(jìn)行缺血再灌注。術(shù)后12h進(jìn)行神經(jīng)功能學(xué)評(píng)分。從術(shù)后第一天開(kāi)始灌胃進(jìn)行藥物干預(yù),每天一次,連續(xù)七天,假手術(shù)組及模型組給予等體積蒸餾水,七天后麻醉處死,迅速斷頭取腦,梗死面積測(cè)定采用TTC染色法,病例形態(tài)學(xué)觀察采用石蠟包埋切片,在高倍顯微鏡下觀察,通過(guò)標(biāo)記腦缺血組織局部CD34表達(dá)來(lái)測(cè)定內(nèi)皮細(xì)胞數(shù)和微血管密度,VEGF、Flk-1、Ang-1、bFGF等生化指標(biāo)的測(cè)定采用免疫組化方法。 結(jié)果：(1)神經(jīng)功能學(xué)評(píng)分：模型組與假手術(shù)組相比,神經(jīng)功能學(xué)評(píng)分明顯升高(P0.01);各用藥組與模型組相比神經(jīng)功能學(xué)評(píng)分降低,有統(tǒng)計(jì)學(xué)意義(P0.05)；丹龍醒腦方各組與尼莫地平組比較無(wú)差異(P0.05)；丹龍醒腦方各組間比較無(wú)明顯差異(p0.05)。(2)腦梗死面積：模型組與假手術(shù)組相比,腦梗死面積明顯增大(P0.01);各用藥組組與模型組相比腦梗死面積降低,有統(tǒng)計(jì)學(xué)意義(P0.05)；丹龍醒腦方大、中劑量組和尼莫地平組有差異(P0.05),丹龍醒腦方小劑量組與尼莫地平組比較無(wú)差異(P0.05)；丹龍醒腦方各組間比較無(wú)明顯差異(P0.05)。(3)缺血海馬區(qū)內(nèi)皮細(xì)胞數(shù)及微血管密度：模型組與假手術(shù)組相比,內(nèi)皮細(xì)胞數(shù)和微血管密度均增加(P0.05);各用藥組與模型組相比內(nèi)皮細(xì)胞數(shù)和微血管密度增加,有統(tǒng)計(jì)學(xué)意義(P0.05);丹龍醒腦方各組和尼莫地平組無(wú)明顯差異(P0.05)；丹龍醒腦方各組間比較無(wú)明顯差異(p0.05)。(4)缺血海馬區(qū)血管新生因子VEGF、Flk-1、Ang-1、bFGF蛋白表達(dá)：模型組與假手術(shù)組相比,VEGF、Flk-1、Ang-1、bFGF蛋白表達(dá)均增強(qiáng)(P0.05)；各用藥組與模型組相比表達(dá)增強(qiáng),有統(tǒng)計(jì)學(xué)意義(P0.05);丹龍醒腦方各組和尼莫地平組相比無(wú)明顯差異(P0.05)；丹龍醒腦方各組間比較無(wú)明顯差異(P0.05)。 結(jié)論：丹龍醒腦方能改善腦缺血再灌注后神經(jīng)功能,縮小梗死面積。腦缺血再灌注損傷后,血管新生因子啟動(dòng),丹龍醒腦方能增加內(nèi)皮細(xì)胞數(shù)及微血管密度,增強(qiáng)血管新生因子VEGF、Flk-1、Ang-1、 bFGF的表達(dá),從而能夠促進(jìn)新生血管的生長(zhǎng)及側(cè)枝循環(huán)的再建立,這可能是其能改善神經(jīng)功能的作用機(jī)制之一。
[Abstract]:Aim: to observe the effects of Danlong Xingnao recipe on cerebral angiogenesis and its factors in rats with cerebral ischemia-reperfusion injury and to explore the effect of Danlong Xingnao recipe on the pathological mechanism of ischemic stroke. Methods: the MCAO method was used to establish the model of cerebral ischemia-reperfusion injury in SD male rats. The surviving rats were randomly divided into 6 groups according to random digital table: large dose group of Danlong Xingnao recipe, middle dose group of Danlong Xingnao prescription, and middle dose group of Danlong Xingnao recipe. Small dose of Danlong Xingnao Fang group, nimodipine positive control group, model group, sham-operation group, 8 rats in each group, except sham-operation group, all the other groups were made model by MCAO method, and the reperfusion was resumed 2 hours after operation, and the external carotid artery was only ligated in the sham-operation group. No ischemia reperfusion. Neurological function score was performed 12 hours after operation. From the first day after operation, drug intervention was performed by intragastric administration, once a day for seven days. Sham-operation group and model group were given distilled water of the same volume. After seven days, the rats were anesthetized and killed, the head was cut off quickly, and the infarct area was measured by TTC staining. The morphology of the patients was observed by paraffin embedded sections, observed under high power microscope, the number of endothelial cells and microvessel density were measured by labeling the local CD34 expression in ischemic brain tissue, and the biochemical indexes such as VEGF,Flk-1,Ang-1,bFGF were determined by immunohistochemical method. Results: (1) the neurological functional score in the model group was significantly higher than that in the sham operation group (P0.01), and the neurological functional score in each medication group was significantly lower than that in the model group (P0.05). There was no difference between Danlong Xingnaofang group and nimodipine group (P0.05), but there was no significant difference between Danlong Xingnao recipe group (p0.05). (2) in cerebral infarction area: model group and sham operation group, The area of cerebral infarction increased significantly (P0.01); compared with the model group, the area of cerebral infarction decreased significantly in each medication group (P0.05); Danlong Xingnao prescription was large, There was significant difference between middle dose group and nimodipine group (P0.05), but there was no difference between small dose group and nimodipine group (P0.05). There was no significant difference in the number of endothelial cells and microvessel density between the three groups (P0.05). (3): compared with the sham operation group, the number of endothelial cells and microvessel density in the ischemic hippocampus in the model group was higher than that in the sham operation group. The number of endothelial cells and microvessel density increased (P0.05); compared with the model group, the number of endothelial cells and microvessel density increased significantly (P0.05); there was no significant difference between Danlong Xingnaofang group and nimodipine group (P0.05). There was no significant difference in the expression of angiogenic factor VEGF,Flk-1,Ang-1,bFGF in ischemic hippocampus between the three groups (p0.05). (4): the expression of VEGF,Flk-1,Ang-1,bFGF protein in model group was higher than that in sham operation group (P0.05); the expression of VEGF,Flk-1,Ang-1,bFGF protein in each medication group was higher than that in model group. There was statistical significance (P0.05); there was no significant difference between the three groups (P0.05); there was no significant difference between the three groups (P0.05). Conclusion: Danlong Xingnao prescription can improve the nerve function and reduce the infarct area after cerebral ischemia and reperfusion. After cerebral ischemia-reperfusion injury, angiogenic factors were activated. Danlong Xingnao Fang could increase the number of endothelial cells and microvessel density, enhance the expression of angiogenic factor VEGF,Flk-1,Ang-1, bFGF, and thus promote the growth of neovascularization and the re-establishment of collateral circulation. This may be one of its mechanisms for improving neural function.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5
本文編號(hào)：2290143
[Abstract]:Aim: to observe the effects of Danlong Xingnao recipe on cerebral angiogenesis and its factors in rats with cerebral ischemia-reperfusion injury and to explore the effect of Danlong Xingnao recipe on the pathological mechanism of ischemic stroke. Methods: the MCAO method was used to establish the model of cerebral ischemia-reperfusion injury in SD male rats. The surviving rats were randomly divided into 6 groups according to random digital table: large dose group of Danlong Xingnao recipe, middle dose group of Danlong Xingnao prescription, and middle dose group of Danlong Xingnao recipe. Small dose of Danlong Xingnao Fang group, nimodipine positive control group, model group, sham-operation group, 8 rats in each group, except sham-operation group, all the other groups were made model by MCAO method, and the reperfusion was resumed 2 hours after operation, and the external carotid artery was only ligated in the sham-operation group. No ischemia reperfusion. Neurological function score was performed 12 hours after operation. From the first day after operation, drug intervention was performed by intragastric administration, once a day for seven days. Sham-operation group and model group were given distilled water of the same volume. After seven days, the rats were anesthetized and killed, the head was cut off quickly, and the infarct area was measured by TTC staining. The morphology of the patients was observed by paraffin embedded sections, observed under high power microscope, the number of endothelial cells and microvessel density were measured by labeling the local CD34 expression in ischemic brain tissue, and the biochemical indexes such as VEGF,Flk-1,Ang-1,bFGF were determined by immunohistochemical method. Results: (1) the neurological functional score in the model group was significantly higher than that in the sham operation group (P0.01), and the neurological functional score in each medication group was significantly lower than that in the model group (P0.05). There was no difference between Danlong Xingnaofang group and nimodipine group (P0.05), but there was no significant difference between Danlong Xingnao recipe group (p0.05). (2) in cerebral infarction area: model group and sham operation group, The area of cerebral infarction increased significantly (P0.01); compared with the model group, the area of cerebral infarction decreased significantly in each medication group (P0.05); Danlong Xingnao prescription was large, There was significant difference between middle dose group and nimodipine group (P0.05), but there was no difference between small dose group and nimodipine group (P0.05). There was no significant difference in the number of endothelial cells and microvessel density between the three groups (P0.05). (3): compared with the sham operation group, the number of endothelial cells and microvessel density in the ischemic hippocampus in the model group was higher than that in the sham operation group. The number of endothelial cells and microvessel density increased (P0.05); compared with the model group, the number of endothelial cells and microvessel density increased significantly (P0.05); there was no significant difference between Danlong Xingnaofang group and nimodipine group (P0.05). There was no significant difference in the expression of angiogenic factor VEGF,Flk-1,Ang-1,bFGF in ischemic hippocampus between the three groups (p0.05). (4): the expression of VEGF,Flk-1,Ang-1,bFGF protein in model group was higher than that in sham operation group (P0.05); the expression of VEGF,Flk-1,Ang-1,bFGF protein in each medication group was higher than that in model group. There was statistical significance (P0.05); there was no significant difference between the three groups (P0.05); there was no significant difference between the three groups (P0.05). Conclusion: Danlong Xingnao prescription can improve the nerve function and reduce the infarct area after cerebral ischemia and reperfusion. After cerebral ischemia-reperfusion injury, angiogenic factors were activated. Danlong Xingnao Fang could increase the number of endothelial cells and microvessel density, enhance the expression of angiogenic factor VEGF,Flk-1,Ang-1, bFGF, and thus promote the growth of neovascularization and the re-establishment of collateral circulation. This may be one of its mechanisms for improving neural function.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 呂威力;邢雪松;趙海;;bFGF對(duì)局灶性腦缺血大鼠腦組織ERK表達(dá)的影響[J];解剖學(xué)研究;2011年02期
2 關(guān)莉;劉微;閆福曼;朱麗娟;周樂(lè)全;李小英;徐進(jìn)文;;補(bǔ)陽(yáng)還五湯對(duì)全腦缺血再灌注不同時(shí)點(diǎn)大鼠皮質(zhì)神經(jīng)細(xì)胞谷氨酸受體2表達(dá)的影響[J];安徽中醫(yī)學(xué)院學(xué)報(bào);2012年04期
3 周小青,劉旺華,李花,羅堯岳,劉建新,陳本美;丹龍醒腦片對(duì)血管性癡呆大鼠學(xué)習(xí)記憶及腦組織興奮性氨基酸影響的實(shí)驗(yàn)研究[J];湖南中醫(yī)學(xué)院學(xué)報(bào);2002年02期
4 周小青,劉旺華,李花,羅堯岳,劉建新,鄧常青;丹龍醒腦片對(duì)血管性癡呆大鼠學(xué)習(xí)記憶及腦組織脂質(zhì)過(guò)氧化和鈣超載的影響[J];湖南中醫(yī)學(xué)院學(xué)報(bào);2002年03期
5 劉旺華,周小青,李花,羅堯岳,劉建新;丹龍醒腦片對(duì)血管性癡呆大鼠血液流變學(xué)影響的實(shí)驗(yàn)研究[J];湖南中醫(yī)學(xué)院學(xué)報(bào);2002年04期
6 馬中建;劉旺華;李花;毛平平;周小青;何倩;;丹龍醒腦片對(duì)大鼠腦缺血再灌注后神經(jīng)細(xì)胞凋亡的影響[J];湖南中醫(yī)藥大學(xué)學(xué)報(bào);2008年03期
7 何倩;李花;周小青;劉旺華;劉建新;張國(guó)民;;丹龍醒腦片對(duì)大鼠腦缺血再灌注后海馬區(qū)AI及Fas/FasL、TNF-α表達(dá)的影響[J];湖南中醫(yī)藥大學(xué)學(xué)報(bào);2009年02期
8 盛西陵,王東明,陳暢,張細(xì)花,許端敏,袁文金,石永英;大鼠心肌缺血再灌注中IL-10、MPO的變化及關(guān)系[J];江西醫(yī)學(xué)院學(xué)報(bào);2003年03期
9 黃愷;姚蘭;黃丹;楊崇哲;李小麗;廖玉華;;急性心肌梗死后1型多聚二磷酸腺苷核糖合成酶的活化與炎癥因子水平的關(guān)系[J];臨床心血管病雜志;2008年05期
10 劉旺華;李花;周小青;何倩;莫莉;張國(guó)民;;丹龍醒腦片對(duì)局灶性腦缺血再灌注大鼠腦梗死體積的影響[J];遼寧中醫(yī)雜志;2008年12期
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