邵氏“五針法”對哮喘模型大鼠a-SMA及FN表達影響的實驗研究
發(fā)布時間：2018-10-14 20:12
【摘要】：目的 本課題通過針刺哮喘模型大鼠“肺俞”、“大椎”、“風門”穴，觀察邵氏“五針法”對各組哮喘模型大鼠肺組織和血清中平滑肌肌動蛋白-a（a-SMA）及纖維連接蛋白（FN）表達的影響以及對肺組織病理變化的影響，探討邵氏“五針法”治療哮喘的機理及其對哮喘模型大鼠氣道重塑的作用機制，為針灸治療哮喘提供科學的實驗依據(jù)。 方法 選取健康雄性清潔級SD大鼠40只（體重140±20g），隨機分成空白對照組、模型對照組、藥物治療組和針刺治療組，每組10只。采用腹腔注射卵蛋白（OVA）致敏，霧化吸入1%的OVA溶液（每日1次，共7次）激發(fā)建立哮喘大鼠模型。針刺治療組于每日激發(fā)后針刺哮喘大鼠“肺俞”(雙)、“大椎”、“風門”(雙)穴治療；藥物治療組于每日激發(fā)后腹腔注射0.05%地塞米松磷酸鈉溶液治療；模型對照組激發(fā)后不做特殊處理；空白對照組腹腔注射及霧化吸入時，用生理鹽水代替卵蛋白進行操作。末次治療后6h內(nèi)水合氯醛麻醉大鼠，取眼球血后脫頸椎處死大鼠，取右中下肺組織，石蠟包埋，制備病理切片，行蘇木精-伊紅（HE）染色及a-SMA、FN肺組織免疫組化染色和血清ELISA檢測，觀察大鼠肺組織形態(tài)學改變以及a-SMA、FN在哮喘模型大鼠肺組織和血清中的表達。 結(jié)果 1.各組大鼠行為體征觀察 模型對照組大鼠與空白對照組比較，出現(xiàn)噴嚏，嗆咳，流涕，喘息，呼吸急促，精神不振，反應遲鈍；空白對照組大鼠行動敏捷，未見異常；藥物治療組、針刺治療組大鼠噴嚏，嗆咳，喘息等癥狀較模型對照組明顯減輕，反應靈敏。 2.各組大鼠肺組織形態(tài)學觀察 模型對照組大鼠氣道黏膜上皮受損脫落，黏膜下層和平滑肌層增生、變形；氣道壁增厚，管腔變窄，管腔內(nèi)大量分泌物；肺間質(zhì)大量炎癥細胞浸潤，肺泡壁破裂變形，肺泡間隔結(jié)構(gòu)紊亂；血管增生，血管管壁增厚。藥物治療組、針刺治療組氣道結(jié)構(gòu)基本完整，肺間質(zhì)炎癥細胞浸潤較少，肺組織結(jié)構(gòu)基本正常�？瞻讓φ战M大鼠氣道結(jié)構(gòu)完整，無明顯脫落和變形，肺間質(zhì)未見炎癥細胞浸潤，肺組織形態(tài)結(jié)構(gòu)正常。 3.各組大鼠肺組織a-SMA的比較 空白對照組大鼠肺組織中少見胞漿呈棕黃色染色的a-SMA陽性細胞；模型對照組大鼠肺組織內(nèi)陽性表達明顯增多，與空白對照組比較，差異具有統(tǒng)計學意義(P＜0.01)；針刺治療組及藥物治療組與模型對照組比較，肺組織內(nèi)陽性表達明顯減少，差異具有統(tǒng)計學意義(P＜0.01)，但仍高于空白對照組；針刺治療組與藥物治療組比較，差異具有統(tǒng)計學意義(P＜0.01)。 4.各組大鼠肺組織FN的比較 空白對照組大鼠肺組織中少見胞漿呈棕黃色染色的FN陽性細胞；模型對照組大鼠肺組織內(nèi)陽性表達明顯增多，與空白對照組比較，，差異具有統(tǒng)計學意義(P＜0.01)；針刺治療組及藥物治療組與模型對照組比較，肺組織內(nèi)陽性表達明顯減少，差異具有統(tǒng)計學意義(P＜0.01)，但仍高于空白對照組；針刺治療組與藥物治療組比較，差異具有統(tǒng)計學意義(P＜0.01)。 5.各組大鼠血清a-SMA的比較 模型對照組與空白對照組相比，大鼠血清中a-SMA濃度顯著增高，差異具有統(tǒng)計學意義(P＜0.01)；針刺治療組及藥物治療組與模型對照組比較，a-SMA濃度明顯降低，差異具有統(tǒng)計學意義(P＜0.01)，但仍高于空白對照組；針刺治療組與藥物治療組比較，差異具有統(tǒng)計學意義(P＜0.01)。 6.各組大鼠血清FN的比較 模型對照組與空白對照組相比，大鼠血清中FN濃度顯著增高，差異具有統(tǒng)計學意義(P＜0.01)；針刺治療組及藥物治療組與模型對照組比較，F(xiàn)N濃度明顯降低，差異具有統(tǒng)計學意義(P＜0.01)，但仍高于空白對照組；針刺治療組與藥物治療組比較，差異具有統(tǒng)計學意義(P＜0.01)。 結(jié)論 1.邵氏“五針法”能夠有效改善哮喘模型大鼠的癥狀和行為體征。 2.邵氏“五針法”能夠有效改善哮喘模型大鼠的肺組織形態(tài)學改變。 3.邵氏“五針法”能夠降低哮喘模型大鼠肺組織中a-SMA和FN的陽性表達。 4.邵氏“五針法”能夠降低哮喘模型大鼠血清中a-SMA和FN的陽性表達，且二者在各組哮喘模型大鼠肺組織和血清中的表達具有一致性，并呈正相關(guān)。 邵氏“五針法”能明顯降低哮喘模型大鼠肺組織和血清中a-SMA及FN的陽性表達，改善氣道重塑，說明降低a-SMA、FN的陽性表達是邵氏“五針法”治療哮喘的作用途徑之一。
[Abstract]:Purpose The objective of this study is to adopt acupuncture and asthma model. Model Rats lung "," Big Vertebra "," Air Door "Point, View Czechsler's Five Effect of Acupuncture on the Expression of Smooth Muscle Actin-a (a-SMA) and Fibronectin (FN) in Lung Tissue and Serum of Rats with Asthma Model Discussion on the Five-year-old Five-year-Five-Year Plan The mechanism of acupuncture therapy for asthma and the mechanism of airway remodeling in asthma model rats. It provides scientific experiments for acupuncture and moxibustion treatment of asthma. Basis Methods Forty SD rats were randomly divided into control group, model control group, drug treatment group and acupuncture treatment group. Treatment group, 10 rats in each group, using intraperitoneal injection of ovalbumin (OVA) to induce sensitivity, atomizing inhalation of 1% solution (once daily, 7 times) Model of asthma rats was established. The acupuncture treatment group was administered daily. Post-excitation needle Staggle asthma rat's lungs" (double)," Dazhui "," air door "(double) acupoint treatment; drug treatment group injected intraperitoneally with 0.05% dexamethasone sodium phosphate solution after daily excitation; no special treatment after excitation of the model control group; when the blank control group was injected and atomized in the abdominal cavity, saline was used for the treatment. The ovalbumin was operated at the end of last treatment. After the last treatment, the rats were anesthetized with chloral hydrate, the rats were sacrificed after taking the eyeball blood, the lung tissue in the right was removed, paraffin was embedded, pathological sections were prepared, hematoxylin-eosin (HE) staining, and a-SMA, FN lung tissue immunohistochemical staining and blood were prepared. The morphological changes of lung tissue of rats and the pulmonary groups of a-SMA and FN in asthmatic rats were observed by ELISA. Weaving and Expression in serum. Results 1. The rats in the control group were compared with the blank control group in the control group of the signs of behavioral signs in each group. The rats were quick and abnormal in action; the drug treatment group, the acupuncture group, the acupuncture group, the group of acupuncture and treatment group, the chronic cough, the wheezing, etc. The symptoms were significantly reduced compared with the model control group, It should be sensitive. 2. The model of lung tissue morphology in each group of rats was observed in the control group, and the mucosal epithelium of the airway was damaged and shed, the submucosa and smooth muscle layer proliferated and deformed, the airway wall was thickened, the tube cavity became narrow, and the tube cavity was large. Keywords secretion; pulmonary interstitial massive inflammatory cell infiltration; alveolar wall rupture Deformation, alveolar space structure disorder, vascular proliferation, vascular wall thickening, drug treatment group, acupuncture treatment group airway structure basically completed There was little infiltration of inflammatory cells in interstitial inflammatory cells, and the structure of lung tissue was basically normal. No inflammatory cell infiltration, lung tissue was found in the interstitial lung Morphologic structure was normal. 3. Compared with the control group of lung tissue a-SMA in each group, the rare cells in the lung tissues of the rats were brown-yellow stained a-SMA positive cells, and the positive expression in the lung tissues of the model control group was obvious. Compared with the blank control group, the difference was statistically significant (P <0.01). Compared with the model control group, the positive expression in the lung tissue was significantly reduced and the difference was statistically significant (P <0.01), but still higher than that of the blank control group. Comparison between treatment group and drug treatment group, difference There was a statistically significant difference (P <0.01). 4. Compared with the control group of lung tissue FN in each group, there were no FN positive cells stained with brown yellow in the lung tissues of the control group; the model control group was in the lung tissue of the rats. There was a significant increase in the positive expression in the lung tissue compared with the control group (P <0.01). Compared with the control group, the positive expression in the lung tissue was significantly reduced, and the difference was statistically significant (P <0.01), but still higher than that in the blank group. Control group; acupuncture treatment group and drug treatment group ratio Compared with the control group, the levels of a-SMA in the serum of rats were significantly higher than that in the control group, and the difference was statistically significant. (P <0.01), the concentration of a-SMA decreased significantly in the acupuncture group and the drug treatment group compared with the model control group (P <0.01). but still higher than blank control group; acupuncture treatment Compared with the control group, the concentration of FN in the serum of rats was significantly higher than that in the control group. The difference was statistically significant (P <0.01); in acupuncture treatment group and drug treatment group, FN concentration decreased significantly compared with the model control group, and the difference was statistically significant (P <0.01). 0. 01), but still higher than empty Comparison and difference between acupuncture treatment group and drug treatment group. with statistics Learning significance (P <0.01)............................................................... the law" can effective improvement of nasal polyps Symptoms and signs of behavior in asthma model rats. effective improvement Asthma model is large Morphological change of lung tissue in rats. The five-stitch "five stitches" can reduce the positive expression of a-SMA and FN in the lung tissues of asthmatic model rats. Law can reduce the temperature and humidity The expression of a-SMA and FN in serum of asthmatic model rats was consistent and positively correlated with the expression of a-SMA and FN in asthmatic model rats. Wu's Five-pin The method can obviously reduce the asthma model
【學位授予單位】：河南中醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R245
[Abstract]:Purpose The objective of this study is to adopt acupuncture and asthma model. Model Rats lung "," Big Vertebra "," Air Door "Point, View Czechsler's Five Effect of Acupuncture on the Expression of Smooth Muscle Actin-a (a-SMA) and Fibronectin (FN) in Lung Tissue and Serum of Rats with Asthma Model Discussion on the Five-year-old Five-year-Five-Year Plan The mechanism of acupuncture therapy for asthma and the mechanism of airway remodeling in asthma model rats. It provides scientific experiments for acupuncture and moxibustion treatment of asthma. Basis Methods Forty SD rats were randomly divided into control group, model control group, drug treatment group and acupuncture treatment group. Treatment group, 10 rats in each group, using intraperitoneal injection of ovalbumin (OVA) to induce sensitivity, atomizing inhalation of 1% solution (once daily, 7 times) Model of asthma rats was established. The acupuncture treatment group was administered daily. Post-excitation needle Staggle asthma rat's lungs" (double)," Dazhui "," air door "(double) acupoint treatment; drug treatment group injected intraperitoneally with 0.05% dexamethasone sodium phosphate solution after daily excitation; no special treatment after excitation of the model control group; when the blank control group was injected and atomized in the abdominal cavity, saline was used for the treatment. The ovalbumin was operated at the end of last treatment. After the last treatment, the rats were anesthetized with chloral hydrate, the rats were sacrificed after taking the eyeball blood, the lung tissue in the right was removed, paraffin was embedded, pathological sections were prepared, hematoxylin-eosin (HE) staining, and a-SMA, FN lung tissue immunohistochemical staining and blood were prepared. The morphological changes of lung tissue of rats and the pulmonary groups of a-SMA and FN in asthmatic rats were observed by ELISA. Weaving and Expression in serum. Results 1. The rats in the control group were compared with the blank control group in the control group of the signs of behavioral signs in each group. The rats were quick and abnormal in action; the drug treatment group, the acupuncture group, the acupuncture group, the group of acupuncture and treatment group, the chronic cough, the wheezing, etc. The symptoms were significantly reduced compared with the model control group, It should be sensitive. 2. The model of lung tissue morphology in each group of rats was observed in the control group, and the mucosal epithelium of the airway was damaged and shed, the submucosa and smooth muscle layer proliferated and deformed, the airway wall was thickened, the tube cavity became narrow, and the tube cavity was large. Keywords secretion; pulmonary interstitial massive inflammatory cell infiltration; alveolar wall rupture Deformation, alveolar space structure disorder, vascular proliferation, vascular wall thickening, drug treatment group, acupuncture treatment group airway structure basically completed There was little infiltration of inflammatory cells in interstitial inflammatory cells, and the structure of lung tissue was basically normal. No inflammatory cell infiltration, lung tissue was found in the interstitial lung Morphologic structure was normal. 3. Compared with the control group of lung tissue a-SMA in each group, the rare cells in the lung tissues of the rats were brown-yellow stained a-SMA positive cells, and the positive expression in the lung tissues of the model control group was obvious. Compared with the blank control group, the difference was statistically significant (P <0.01). Compared with the model control group, the positive expression in the lung tissue was significantly reduced and the difference was statistically significant (P <0.01), but still higher than that of the blank control group. Comparison between treatment group and drug treatment group, difference There was a statistically significant difference (P <0.01). 4. Compared with the control group of lung tissue FN in each group, there were no FN positive cells stained with brown yellow in the lung tissues of the control group; the model control group was in the lung tissue of the rats. There was a significant increase in the positive expression in the lung tissue compared with the control group (P <0.01). Compared with the control group, the positive expression in the lung tissue was significantly reduced, and the difference was statistically significant (P <0.01), but still higher than that in the blank group. Control group; acupuncture treatment group and drug treatment group ratio Compared with the control group, the levels of a-SMA in the serum of rats were significantly higher than that in the control group, and the difference was statistically significant. (P <0.01), the concentration of a-SMA decreased significantly in the acupuncture group and the drug treatment group compared with the model control group (P <0.01). but still higher than blank control group; acupuncture treatment Compared with the control group, the concentration of FN in the serum of rats was significantly higher than that in the control group. The difference was statistically significant (P <0.01); in acupuncture treatment group and drug treatment group, FN concentration decreased significantly compared with the model control group, and the difference was statistically significant (P <0.01). 0. 01), but still higher than empty Comparison and difference between acupuncture treatment group and drug treatment group. with statistics Learning significance (P <0.01)............................................................... the law" can effective improvement of nasal polyps Symptoms and signs of behavior in asthma model rats. effective improvement Asthma model is large Morphological change of lung tissue in rats. The five-stitch "five stitches" can reduce the positive expression of a-SMA and FN in the lung tissues of asthmatic model rats. Law can reduce the temperature and humidity The expression of a-SMA and FN in serum of asthmatic model rats was consistent and positively correlated with the expression of a-SMA and FN in asthmatic model rats. Wu's Five-pin The method can obviously reduce the asthma model
【學位授予單位】：河南中醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R245
【參考文獻】
相關(guān)期刊論文 前10條
1 朱文蓮,劉仁權(quán);艾灸大椎穴對免疫低下小鼠巨噬細胞吞噬功能的影響[J];北京中醫(yī)藥大學學報;2005年01期
2 王延玲;;隔姜灸治療哮喘臨床療效觀察[J];上海針灸雜志;2012年03期
3 邵素菊;李鴻章;李會超;王磊瓊;;“天灸”治療肺系病證80例[J];陜西中醫(yī);2007年04期
4 譚程;趙吉平;張昶;;近十年針刺治療支氣管哮喘的臨床及實驗研究現(xiàn)狀[J];針刺研究;2011年04期
5 吳兆利;劉自力;張慶榮;;針刺足三里對脾虛哮喘大鼠TGF-β和GM-CSF濃度的影響[J];中國中醫(yī)藥信息雜志;2009年12期
6 李s
本文編號：2271520
本文鏈接：http://sikaile.net/yixuelunwen/mazuiyixuelunwen/2271520.html
最近更新
教材專著
