【摘要】：目的觀察攜帶人肝細胞生長因子基因的重組腺病毒(Ad-HGF)蛛網(wǎng)膜下腔注射后腦動脈粥樣硬化老齡大鼠大腦皮層血流量變化。方法 60只大鼠中隨機取45只造模,采用高血壓合并高脂飼料喂養(yǎng)制備動脈粥樣硬化模型。取建模成功的40只大鼠,隨機分為模型組、高劑量組、中劑量組、低劑量組。高、中、低劑量組分別給予5×10~8、2.5×10~8、1.25×10~8pfu/10μL的Ad-HGF,藥物經(jīng)小腦延髓池注入至蛛網(wǎng)膜下腔。模型組給予等體積的生理鹽水。分別于給藥后第10、20、30天抽取各組大鼠腦脊液,采用ELISA法檢測腦脊液中的HGF;將各組大鼠以10%水合氯醛腹腔注射麻醉,于大腦中動脈主要供血區(qū)采用激光多普勒血流儀測算腦血流量,以血流儀顯示的電壓皮伏特值(PU)代表大腦皮層血流量。結(jié)果低、中、高劑量組Ad-HGF給藥后第10、20、30天腦脊液中HGF水平逐漸降低,且均高于模型組(P均0.05)。中、高劑量組給藥后相同時間腦脊液中HGF水平高于低劑量組(P均0.05)。低、中、高劑量組Ad-HGF給藥后不同時間大鼠大腦皮層血流量均高于模型組(P均0.05)。低、中、高劑量組Ad-HGF給藥后第20、30天大鼠大腦皮層血流量高于給藥第10天(P均0.05)。中、高劑量組給藥后相同時間大腦皮層血流量高于低劑量組(P均0.05)。結(jié)論 Ad-HGF蛛網(wǎng)膜下腔注射可提高腦動脈粥樣硬化老齡大鼠大腦皮層血流量,作用與給藥劑量、給藥時間有關(guān)。
[Abstract]:Objective to observe the changes of cerebral cortical blood flow after subarachnoid injection of recombinant adenovirus (Ad-HGF) carrying human hepatocyte growth factor gene in aged rats with cerebral atherosclerosis. Methods Forty-five rats were randomly selected and fed with high fat diet to establish atherosclerosis model. Forty rats were randomly divided into three groups: model group, high dose group, middle dose group and low dose group. The high, medium and low dose groups were given 5 脳 10 ~ (8) (2.5 脳 10 ~ (-8) ~ (1.25 脳 10~8pfu/10) 渭 L Ad-HGF, into the subarachnoid space via the cerebellar medulla oblongata cistern. The model group was given saline of equal volume. Cerebrospinal fluid (CSF) was extracted from each group on day 10, 2030 after administration. HGF; in CSF was detected by ELISA method. The rats in each group were anesthetized with 10% chloral hydrate intraperitoneally, and the cerebral blood flow was measured by laser Doppler flow meter in the main blood supply area of the middle cerebral artery. Cortical blood flow was represented by the voltage Pivot value (PU) displayed by the blood flow meter. Results the levels of HGF in cerebrospinal fluid (CSF) in low, medium and high dose groups were gradually decreased at day 10, 20 and 30 after administration of Ad-HGF, and were higher than those in model group (P 0.05). The level of HGF in cerebrospinal fluid of middle and high dose group was higher than that of low dose group at the same time after administration (all P 0. 05). The cortical blood flow in the low, middle and high dose groups was higher than that in the model group at different time after Ad-HGF administration (all P 0. 05). The cerebral cortical blood flow in the low, middle and high dose groups was higher than that in the 10th day after administration of Ad-HGF (P < 0. 05). The cortical blood flow in the middle and high dose group was higher than that in the low dose group at the same time (P 0.05). Conclusion Ad-HGF subarachnoid injection can increase cerebral cortical blood flow in aged rats with atherosclerosis, and the effect is related to dosage and time of administration.
【作者單位】： 新鄉(xiāng)醫(yī)學院第一附屬醫(yī)院;
【基金】：河南省醫(yī)學科技攻關(guān)計劃項目(201401012)
【分類號】：R743.3
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本文編號：2243731