【摘要】：研究表示AD患者的腦內(nèi)鐵水平異常升高,這可能與腦組織內(nèi)鐵代謝及蛋白表達異常,腦內(nèi)自由基和炎癥因子水平升高,外周鐵代謝失衡及臟器功能衰退有關(guān)。降低腦鐵的含量是防治AD的一個方法之一。鐵螯合劑如去鐵胺可以明顯改善AD的臨床癥狀,但其副作用也十分的明顯,且口服效果較差,因此吸收好,生物利用度高的中藥成為治療AD鐵超載的首選,具有巨大的潛力。中醫(yī)認為AD是本虛標實之癥,其中腎虛精虧,氣血不足為本,痰瘀互結(jié),濁毒內(nèi)生為標。因此,AD的中醫(yī)治療應以補腎健脾,益氣養(yǎng)血為本。淫羊藿具補腎填精、生精養(yǎng)髓之功效,黃芪具補氣健脾、行血化瘀之功效,葛根具有清熱排毒之功效三藥合用,共同作用,起滋補肝腎、補脾養(yǎng)血、活血化瘀、清熱解毒、健腦益智的功效。從淫羊藿,黃芪,葛根提取的有效成分具有清除自由基,降低炎癥反應等多個功效,可以從多方面抑制鐵超載,揚長避短。因此本課題以淫羊藿,黃芪,葛根有效成分進行組方對AD腦鐵超載的干預和作用切入,為中藥防治AD提供科學的依據(jù)�，F(xiàn)代藥理對這三種成分治療AD的機制進行了大量研究,但多數(shù)從神經(jīng)遞質(zhì)含量,氧化應激水平,Aβ代謝等方面著手,對腦鐵超載關(guān)注不足。因此,本課題以鐵代謝為切入點,以補腎補精,祛痰化瘀,補血益氣為原則,將淫羊藿,黃芪,葛根三種藥物的有效成分進行組方,以APP/PS1雙轉(zhuǎn)基因小鼠為動物模型,觀察藥物對動物模型的治療療效,以及腦鐵的干預效果,并深入探討其可能的機制,為AD的藥物治療開辟一個新的方向,為中藥防治AD提供科學的依據(jù)。第一部分淫羊藿、黃芪、葛根有效成分組方對阿爾茨海默病轉(zhuǎn)基因小鼠腦鐵含量的氧化應激、炎性因子水平影響的研究目的:觀察淫羊藿,黃芪,葛根及其有效成分組方對APP/PS1雙轉(zhuǎn)基因小鼠腦鐵水平以及氧化應激、炎性因子水平的影響。方法:六月齡雄性app/ps1雙轉(zhuǎn)基因小鼠60只,隨機分為模型組,復方組,淫羊藿組,黃芪組,葛根組,dfo組,其中復方組灌胃給予淫羊藿苷,黃芪甲苷,葛根素組方,淫羊藿組灌胃給予淫羊藿苷,黃芪組灌胃給予黃芪甲苷,葛根組灌胃給予葛根素,dfo組灌胃給予dfo(去鐵胺),以上灌胃時長均為三個月。c57bl/6j小鼠10只作為陰性對照組,模型組及對照組給與等量生理鹽水灌胃3個月。灌胃結(jié)束后,灌流后快速取出大腦皮層及海馬,一側(cè)皮層用火焰原子吸收法測定鐵元素含量,海馬用等離子質(zhì)譜法(icp-ms)測定鐵元素含量,另一側(cè)皮層用生化法測定氧化因子(sod、mda、gsh-px)及炎性因子(il-1β、il6、tnf-α)的含量。結(jié)果:火焰原子吸收法結(jié)果顯示模型組小鼠與對陰性照組小鼠相比皮質(zhì)鐵含量明顯增加(p0.05),復方組小鼠與模型組小鼠相比皮質(zhì)鐵含量明顯下降(p0.05);等離子質(zhì)譜法(icp-ms)顯示模型組小鼠與對陰性照組小鼠相比海馬鐵含量明顯增加(p0.05),復方組小鼠與模型組小鼠相比海馬鐵含量明顯下降(p0.05);elisa法結(jié)果顯示模型組小鼠與對陰性照組小鼠相比皮質(zhì)il-1β、il6、tnf三者含量明顯增加(p0.05),復方組小鼠與模型組小鼠相比皮質(zhì)il-1β、il-6、tnf三者含量明顯下降(p0.05);模型組小鼠與對陰性照組小鼠相比皮質(zhì)mda含量明顯增加(p0.05),復方組小鼠與模型組小鼠相比皮質(zhì)mda含量明顯下降(p0.05)。模型組小鼠與對陰性照組小鼠相比皮質(zhì)sod、gsh-px兩者含量明顯下降(p0.05),復方組小鼠與模型組小鼠相比皮質(zhì)sod、gsh-px兩者含量明顯增加(p0.05)。第二部分淫羊藿、黃芪、葛根有效成分組方對阿爾茨海默病轉(zhuǎn)基因小鼠血清鐵、外周組織器官鐵水平及氧化應激水平的影響目的:本實驗以app/ps1雙轉(zhuǎn)基因小鼠為動物模型,來觀察淫羊藿,黃芪,葛根有效成分組方對小鼠血清鐵、外周組織器官鐵水平及氧化應激水平的影響,進而探討對淫羊藿、黃芪、葛根有效成分組分對腦鐵干預是否與改善外周鐵代謝失衡及臟腑功能衰退相關(guān)。方法:雄性的六月齡雄性app/ps1雙轉(zhuǎn)基因小鼠60只,隨機分為模型組,復方組,淫羊藿組,黃芪組,葛根組,dfo組,其中復方組灌胃給予淫羊藿苷,黃芪甲苷,葛根素組方,淫羊藿組灌胃給予淫羊藿苷,黃芪組灌胃給予黃芪甲苷,葛根組灌胃給予葛根素,dfo組灌胃給予dfo(去鐵胺),以上灌胃操作1次/日,時長均為三個月。中藥淫羊藿,黃芪,葛根3種藥物的有效成分按照比例3:2:2,即淫羊藿苷120mg/kg,黃芪甲苷80mg/kg,,葛根素80mg/kg體重給予小鼠灌胃。c57bl/6j小鼠10只作為陰性對照組,模型組及對照組給與等量生理鹽水灌胃3個月。采用火焰原子吸收光譜技術(shù)測定肝臟,腎臟,心臟,脾臟和肺臟的鐵含量。酶聯(lián)免疫吸附試驗法(elisa)檢測小鼠血清鐵(si),鐵蛋白(fn)和轉(zhuǎn)鐵蛋白(tf)水平。生化試劑盒檢測小鼠肝臟組織,腎臟組織,心臟組織,肺臟組織丙二醛(mda)含量,超氧化物歧化酶(sod)活力,谷胱甘肽過氧化物酶(gsh-px)活力。結(jié)果:火焰原子吸收光譜技術(shù)顯示,與對照組小鼠相比,模型組小鼠外周鐵含量明顯升高(p0.05);與ad模型組相比,黃芪組、葛根組、復方組和dfo組鐵含量降低(p0.05);復方組與各單方組各組小鼠相比,鐵含量明顯降低(p0.05),dfo組與復方組無明顯差異(p0.05)。結(jié)果提示app/ps1雙轉(zhuǎn)基因小鼠腎臟組織,脾臟組織,心臟組織和肺臟組織等外周組織內(nèi)存在鐵超載,復方組可以顯著降低各組織器官的鐵含量,其效果優(yōu)于單方組。與對照組小鼠相比,ad模型組小鼠si和fn含量明顯升高(p0.05),tf水平明顯下降;與ad模型組相比,復方組和dfo組si和fn含量降低,tf水平升高;復方組與各單方組各組小鼠相比,si和fn含量降低,tf水平升高,表明復方效果優(yōu)于單方;dfo組與復方組無明顯差異(p0.05)。為了深入探討淫羊藿、黃芪、葛根有效成分組方對ad雙轉(zhuǎn)基因模型小鼠外周組織抗氧化能力的影響,本部分實驗分別檢測了ad模型雙轉(zhuǎn)基因小鼠肝臟組織,腎臟組織,心臟組織和肺臟組織內(nèi)的sod、mda、gsh-px含量。與對照組小鼠相比,ad模型組肝臟、腎臟、心臟sod、gsh-px活力均有所下降,mda含量有所上升(p0.05);與ad模型組相比,淫羊藿組、黃芪組、葛根組、復方組和dfo組肝臟、腎臟、心臟中sod、gsh-px活力均有所升高(p0.05),mda含量有所降低,復方組與各單方組各組小鼠相比,sod、gsh-px活力均有所升高(p0.05),mda含量明顯降低(p0.05),復方組優(yōu)于單方組;dfo組與復方組無明顯差異(p0.05)。第三部分淫羊藿、黃芪、葛根有效成分組方對阿爾茨海默病轉(zhuǎn)基因小鼠血清epo及紅細胞生成的影響目的:本實驗以app/ps1雙轉(zhuǎn)基因小鼠為動物模型,來觀察淫羊藿,黃芪,葛根有效成分組方對小鼠血清epo生成及紅細胞的影響,并對其引起腦鐵超載的可能機制進行探討。方法:給藥處理同前部分實驗。分為模型組,復方組,淫羊藿組,黃芪組,葛根組,dfo組,c57bl/6j小鼠作為陰性對照組。從各組10只小鼠中隨機選取4只,20%烏拉坦腹腔麻醉后快速摘眼球取血約1ml,靜置后,冰上靜置后4℃,3000r/min,離心15min,得血清,分裝后-80℃保存用于小鼠血清促紅細胞生成素(epo)的檢測。各組另取小鼠3只,麻醉后快速摘眼球取血至加有肝素抗凝的ep管中,上下顛倒混勻,低溫保存,迅速進行血常規(guī)檢測。結(jié)果:與對照組小鼠相比,ad模型組小鼠epo含量明顯降低(p0.05),結(jié)果提示app/ps1雙轉(zhuǎn)基因小鼠血清中存在epo生成減少。與ad模型組相比,淫羊藿組,黃芪組、葛根組、復方組和dfo組腦epo含量明顯升高(p0.05),復方組和dfo組與各單方組各組小鼠相比,epo含量上升明顯(p0.05)。小鼠血常規(guī)紅細胞(rbc)檢測結(jié)果顯示:與對照組小鼠相比,ad模型組小鼠紅細胞數(shù)量明顯減少(p0.05),與ad模型組相比,淫羊藿組,黃芪組、葛根組、復方組和dfo組紅細胞數(shù)量有所增加(p0.05),復方組和dfo組與各單方組各組小鼠相比,紅細胞數(shù)量有明顯增加(p0.05);復方組和dfo組相比無明顯差異(p0.05)。血常規(guī)血紅蛋白(hb)檢測結(jié)果顯示:淫羊藿組,黃芪組、葛根組、復方組和dfo組與ad模型組相比血紅蛋白含量有所增加(p0.05),其中復方組和dfo組明顯增加(p0.05),與c57組小鼠相比,ad模型組小鼠血紅蛋白含量明顯減少(p0.05)。紅細胞壓積(hct)結(jié)果顯示,相比對照組,模型組小鼠的hct/百分比減少(p0.05),與模型組相比,復方組hct水平明顯上升(p0.05)。ad模型組小鼠白細胞(wbc)數(shù)量增加(p0.05),淫羊藿組,黃芪組、葛根組、復方組和dfo組白細胞數(shù)量有所降低(p0.05)。其中復方組和dfo組相比無明顯差異,使白細胞數(shù)量下降的較為明顯。結(jié)果提示,ad模型小鼠體內(nèi)有炎癥的存在,經(jīng)藥物治療后體內(nèi)炎性水平好轉(zhuǎn)。第四部分淫羊藿、黃芪、葛根有效成分組方對鐵超載阿爾茨海默病轉(zhuǎn)基因小鼠血脂的影響目的:研究淫羊藿,黃芪,葛根三者有效成分組方對血脂的影響,并進一步探討血脂代謝紊亂對腦鐵升高的影響。方法:六月齡雄性app/ps1雙轉(zhuǎn)基因小鼠,隨機分為模型組,復方組,淫羊藿組,黃芪組,葛根組,dfo組,其中復方組灌胃給予淫羊藿,黃芪,葛根組方,淫羊藿組灌胃給予淫羊藿,黃芪組灌胃給予黃芪,葛根組灌胃給予葛根,dfo組灌胃給予dfo(去鐵胺),以上灌胃時長均為三個月。c57bl/6j小鼠作為陰性對照組,模型組及對照組給與等量生理鹽水灌胃3個月。各組隨機選取3只,末次給藥結(jié)束后,禁食禁水16小時,20%烏拉坦麻醉摘眼球取血后,4℃,3000r/min,離心15分鐘獲得血清后,用全自動生化分析儀測得各組的甘油三酯(tg),總膽固醇(tc),高密度脂蛋白(hdl)低密度脂蛋白(ldl)的含量。采用rt-pcr對肝臟hepcidinmrna表達進行測定。結(jié)果:與對照組小鼠相比,模型組小鼠tg和ldl含量明顯增加(p0.05),hdl含量有所下降(p0.05),tc含量無明顯變化(p0.05);與模型組小鼠相比,復方組小鼠tg和ldl含量明顯下降,hdl含量有所增加(p0.05),tc含量無明顯變化(p0.05)。結(jié)論:app/ps1雙轉(zhuǎn)基因小鼠的海馬及皮質(zhì)的鐵水平均顯著升高,血清鐵水平升高,血清鐵轉(zhuǎn)運能力降低,外周器官組織內(nèi)存在鐵超載,小鼠體內(nèi)炎性因子水平及自由基含量均升高,血清epo水平下降,血常規(guī)異常,血脂異常。應用淫羊藿,黃芪,葛根有效成分組方可以有效的改善轉(zhuǎn)基因小鼠腦鐵超載現(xiàn)象,使其腦內(nèi)的炎癥水平降低,氧化反應減少,且降低了外周鐵含量,有效改善血管及外周器官的氧化損傷,提高AD雙轉(zhuǎn)基因模型小鼠血清EPO的水平,增加RBC數(shù)量,Hb和HTC含量增加,促進鐵利用,降低鐵超載,降低血脂,從而達到抗動脈粥樣硬化,改善血液循環(huán),防止外周鐵沉積進而改善腦鐵超載的作用。
[Abstract]:The abnormal increase in iron levels in the brain of AD patients may be related to the abnormal iron metabolism and protein expression in the brain, the increase of free radicals and inflammatory factors in the brain, the imbalance of peripheral iron metabolism and the deterioration of organ function. The reduction of iron content in the brain is one of the ways to prevent and control AD. Iron chelating agent, such as iron amine, can obviously improve AD. The clinical symptom, but its side effect is also very obvious, and the oral effect is poor, so good absorption, high bioavailability of traditional Chinese medicine has become the first choice for the treatment of AD iron overload, with great potential. Traditional Chinese medicine thinks that AD is the symptom of this deficiency, among which the deficiency of kidney is deficient, Qi and blood is insufficient, phlegm and blood stasis, and the endogeny of turbidity and poison is the standard. Therefore, the TCM treatment of AD It should make up the kidney and invigorate the spleen, nourish the blood and nourish the blood as the basis. The herba Epimedium has the efficacy of nourishing the kidney and nourishing the marrow. The Astragalus membranaceus has the effect of enriching the spleen and invigorating the spleen, making the blood stasis and removing blood stasis. The Pueraria has three drugs combined with the efficacy of clearing heat and detoxification. It plays the function of nourishing the liver and kidney, nourishing the spleen and nourishing blood, promoting blood circulation and removing stasis, clearing heat and detoxifying the brain and improving the wisdom of the brain. Extract from Epimedium, Astragalus Astragalus and pueraria lobata The effective components of the active ingredients can remove the free radical and reduce the inflammatory reaction and so on. It can inhibit the overloading of iron from many aspects, so the effective components of epimedium, Astragalus membranaceus and pueraria lobata are used for the intervention and effect of the AD brain iron overload, which provides scientific basis for the prevention and treatment of AD by traditional Chinese medicine. The mechanism of AD treatment has been a great deal of research, but most from the content of neurotransmitters, the level of oxidative stress, A beta metabolism and so on, it is not enough to pay attention to the overloading of the brain iron. Therefore, this subject takes iron metabolism as the breakthrough point, with the principle of supplementing the kidney, removing phlegm and removing blood stasis, enriching blood and enriching qi, and organizing the effective components of the three kinds of drugs, such as epimedium, astragalus, and pueraria lobata. APP/PS1 double transgenic mice were used as animal models to observe the therapeutic effect of drugs on animal models and the effect of cerebral iron intervention, and to explore the possible mechanism in order to open a new direction for the drug treatment of AD and to provide scientific basis for the prevention and control of AD by traditional Chinese medicine. Study on the effects of oxidative stress on iron content in the brain of transgenic mice with Alzheimer's disease and the effect of inflammatory factors: the effects of epimedium, Astragalus membranaceus, pueraria root and its effective components on the level of iron brain, oxidative stress and inflammatory factors in APP/PS1 double transgenic mice. Methods: 6 month old male app/ps1 double transgenic mice were randomly divided into two groups. The model group, the compound group, the Epimedium group, the Astragalus group, the puerarin group and the DFO group, of which the compound group was given the icariin, astragaloside, puerarin group, the Epimedium group gavage with icariin, the Astragalus group gavage with Huang Qijia glycoside, the GAGG group gavage puerarin, and the group DFO was given DFO (de ferric amine), and the length of the gavage was three months.C. 10 57bl/6j mice were used as negative control group. The model group and the control group were given the same amount of saline for 3 months. After the end of the gavage, the cerebral cortex and hippocampus were quickly removed. The iron content was measured by flame atomic absorption spectrometry. The content of iron was determined by plasma mass spectrometry (ICP-MS) in the hippocampus, and the other side of the cortex was used for biochemistry. Results: the contents of SOD, MDA, GSH-Px and inflammatory factors (IL-1 beta, IL6, tnf- alpha) were measured. Results: the results of flame atomic absorption spectrometry showed that the content of iron in the model group was significantly increased compared with the negative group (P0.05), and the content of iron in the compound group was significantly lower than that in the model group (P0.05), and the plasma mass spectrometry (icp-m) (icp-m). S) showed that the iron content in the hippocampus of the model mice was significantly increased compared with the negative mice (P0.05). The content of iron in the hippocampus of the compound group mice was significantly lower than that in the model group (P0.05). The results of the ELISA method showed that the contents of the model mice and the negative group were significantly increased (P0.05) in the cortex of IL-1 beta, IL6 and TNF three (P0.05), and the mice in the compound group were compared with those in the negative group. Compared with the model group, the content of IL-1 beta, IL-6, TNF three decreased significantly (P0.05), and the cortical MDA content of the model mice was significantly increased (P0.05) compared with the negative group mice (P0.05). The content of the cortical MDA in the compound group mice was significantly lower than that in the model group (P0.05). The mice in the model group and the negative irradiated mice were compared with the cortical SOD and GSH-Px. The content of the compound group mice was significantly increased (P0.05). The contents of SOD and GSH-Px in the mice were significantly increased (P0.05). The effect of the second part of epimedium, Astragalus membranaceus and Radix Puerariae on the serum iron, the level of iron and oxidative stress in the peripheral tissues of transgenic mice with Alzheimer's disease The gene mice were animal models to observe the effect of the effective components of epimedium, Astragalus membranaceus and pueraria lobata on the iron levels and the level of iron and oxidative stress in the peripheral tissue, and then to explore whether the intervention of the effective components of epimedium, astragalus, and pueraria lobata on the imbalance of the peripheral iron metabolism and the function decline of the viscera was related to the effect of the effective components of epimedium, astragalus, and pueraria. 60 male app/ps1 male app/ps1 double transgenic mice were randomly divided into model group, compound group, Epimedium group, Astragalus group, puerarin group, group DFO, of which the compound group gavage was given icariin, astragaloside, puerarin group, Epimedium group gavage to icariin, Astragalus group gavage to astragaloside, puerarin group gavage to puerarin, D Group fo was given DFO (de ferric amine) for 1 times a day for three months. The effective components of the 3 drugs of epimedium herb, Astragalus membranaceus and pueraria lobata were in proportion to 3:2:2, namely, icariin 120mg/kg, astragaloside 80mg/kg, and 80mg/kg weight of puerarin to mice,.C57bl/6j mice were given as negative control group, model group and control The content of iron in liver, kidney, heart, spleen and lung was measured by flame atomic absorption spectroscopy for 3 months. The serum iron (SI), ferritin (FN) and transferrin (TF) level of mice were detected by enzyme linked immunosorbent assay (ELISA). The liver tissue, kidney tissue and heart tissue of mice were detected by biochemical kit. The content of malondialdehyde (MDA), superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity in lung tissues. Results: flame atomic absorption spectrometry showed that the content of peripheral iron in the model group was significantly higher than that of the control group (P0.05). Compared with the ad model group, the content of iron in the Astragalus group, the Pueraria group, the compound group and the DFO group decreased. The iron content of the compound group was significantly lower (P0.05) than the mice in each group (P0.05), and there was no significant difference between the DFO group and the compound group (P0.05). The results suggested that the renal tissue, the spleen tissue, the heart tissue and the lung tissues of the app/ps1 double transgenic mice were overloaded with iron, and the compound group could significantly reduce the iron content in the tissues and organs. Compared with the control group, the content of Si and FN in the ad model group increased significantly (P0.05) and the level of TF decreased obviously. Compared with the ad model group, the Si and FN content of the compound group and DFO group decreased and the TF level increased, and the content of Si and FN was lower and the level of FN in the compound group was compared with the mice in each group. The compound effect was superior to the compound group. There was no significant difference between the DFO group and the compound group (P0.05). In order to investigate the effect of epimedium, Astragalus membranaceus and Radix Puerariae on the antioxidant capacity of the peripheral tissue of ad double transgenic mice, the experiment was carried out to detect the SOD, MDA in the liver tissue, the renal tissue, the heart tissue and the lung tissues of the ad model double transgenic mice. GSH-Px content. Compared with the control group, the liver, kidney, SOD, GSH-Px activity of the heart of the ad model group decreased and the MDA content increased (P0.05). Compared with the ad model group, the livers of epimedium, astragalus, kudzu, the liver, the kidneys, the kidneys, the heart, the SOD and GSH-Px activity of the heart were all elevated (P0.05), the MDA content was reduced, and compound group was reduced. The activity of SOD and GSH-Px increased (P0.05) and the content of MDA decreased significantly (P0.05) in each group, and the compound group was superior to the unilateral group; there was no significant difference between the group DFO and the compound group (P0.05). The effect of the third part of epimedium, Astragalus membranaceus and Radix Puerariae on the formation of serum EPO and erythrocyte in the Alzheimer's disease transgenic mice: In this experiment, app/ps1 double transgenic mice were used as animal models to observe the effect of epimedium, Astragalus membranaceus and Radix Puerariae on the formation of EPO and red blood cells in the serum of mice, and to discuss the possible mechanism of the overloading of brain iron. Group, group DFO and c57bl/6j mice were selected as negative control group. 4 of 10 mice were randomly selected from each group. 20% uran abdominal anesthesia was used to pick up the blood for 1ml. After statically placed, the serum was placed at 4, 3000r/min, centrifugation and 15min. The serum was stored at -80 C for the detection of erythropoietin (EPO) in mice serum. Another 3 mice were taken from each group. Only after anaesthesia, the blood was picked up quickly in the EP tube with heparin anticoagulant, mixed up and down and mixed up and down, preserved at low temperature, and carried out the blood test quickly. Results: compared with the control group, the content of EPO in the ad model mice decreased significantly (P0.05). The results suggested that the EPO generation in the serum of the app/ps1 double transgenic mice decreased. Compared with the ad model group, the prostitution of the mice was more than that of the ad model group. The content of EPO in the brain of the group of Astragalus membranaceus, the radix puerariae, the compound group and the DFO group increased significantly (P0.05). The content of EPO in the compound group and the DFO group increased significantly (P0.05). The test results of the blood routine red blood cell (RBC) in mice showed that the number of red blood cells in the mice of the ad model group decreased significantly (P0.05), and the ad model was compared with the control group. The number of red blood cells in the herba Epimedium group, the Astragalus group, the Puerariae group, the compound group and the DFO group increased (P0.05). The number of red blood cells in the compound group and the DFO group was significantly increased (P0.05) compared with the mice in each group (P0.05). The blood routine hemoglobin (HB) detection results showed that the Epimedium group and the Astragalus group were the result of the blood routine hemoglobin (HB) test. The hemoglobin content of the Pueraria group, the compound group and the DFO group increased (P0.05) compared with the ad model group, and the compound group and the DFO group increased significantly (P0.05). Compared with the C57 group, the hemoglobin content of the ad model mice decreased significantly (P0.05). The result of the hematocrit (HCT) showed that the percentage of hct/ in the model group decreased (p0.0) compared with the control group (p0.0). 5), compared with the model group, the HCT level of the compound group increased significantly (P0.05) in the.Ad model group, the number of white blood cells (WBC) increased (P0.05). The number of white blood cells in the herba Epimedium group, the Astragalus group, the Pueraria group, the compound group and the DFO group decreased (P0.05). The number of white cells in the compound group and the DFO group was not significantly different, and the number of leukocytes decreased obviously. The results suggested ad. The effect of the fourth part of epimedium, Astragalus membranaceus and Radix Puerariae on the blood lipid of transgenic mice with Alzheimer's disease: the effect of the effective components of epimedium, Astragalus and kudzu on the blood lipid, and to further explore the blood lipid generation. Methods: 6 month old male app/ps1 double transgenic mice were randomly divided into model group, compound group, Epimedium group, Astragalus group, Pueraria group and DFO group. The compound group was given Epimedium with epimedium, Radix Astragali, Puerariae group, Epimedium gavage to Herba epimedium, Astragalus group gavage to Astragalus membranaceus, Pueraria group gavage to pueraria. The root, group DFO was given DFO (deferamine), the length of.C57bl/6j mice was three months as the negative control group. The model group and the control group were given the same amount of saline for 3 months. 3 rats were randomly selected. After the last administration, the fasting was 16 hours, and 20% uranan was taken from the eyeball, 4, 3000r/min, and 15 minutes. After the serum was obtained, the content of triglyceride (TG), total cholesterol (TC) and high density lipoprotein (HDL) low density lipoprotein (LDL) were measured by the automatic biochemical analyzer. The expression of hepcidinmrna in the liver was measured by RT-PCR. Results: compared with the control group, the content of TG and LDL in the model group increased significantly (P0.05), and the HDL content was lower. There was no significant change in the content of TC (P0.05) in P0.05 (P0.05). Compared with the model group, the content of TG and LDL in the compound group decreased significantly, and the HDL content increased (p0.0).
【學位授予單位】：河北醫(yī)科大學
【學位級別】：博士
【學位授予年份】：2016
【分類號】：R749.16

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