發(fā)布時間：2018-07-17 19:45

【摘要】：目的：研究去甲腎上腺素（Norepinephrine、NE)、硝普鈉（Sodium nitroprusside、SNP)及酒精（Alcohol）對麻醉實驗性糖尿病大鼠腸系膜微動、靜脈血管反應(yīng)性的影響。 方法:正常雄性SD大鼠（220～250g）78只，誘導(dǎo)實驗性糖尿病大鼠（STZ）78只。采用BI-2000醫(yī)學(xué)圖像分析系統(tǒng)，采集麻醉大鼠腸系膜微循環(huán)顯微圖像，比較觀察局部滴加不同濃度NE(10-12～10-4mol/L)、SNP(10-10～10-4mol)、酒精（0.025%～3.2%）及灌胃不同濃度酒精（0.375%～48%、5min）對實驗性糖尿病大鼠腸系膜微血管直徑的影響，并且觀察酚妥拉明（Phentolamine）對NE收縮血管作用的影響及左旋硝基精氨酸甲酯（L-NAME）對SNP舒張血管的影響。 1.第一部分，局部分別滴加不同濃度NE(10-12～10-4mol/L)于對照組及實驗性糖尿病大鼠腸系膜微血管，觀察NE對大鼠腸系膜微動、靜脈血管反應(yīng)性的影響；酚妥拉明孵育1min后，NE對大鼠腸系膜微動、靜脈血管收縮作用的影響。 2.第二部分，局部分別滴加不同濃度SNP(10-10～10-4mol/L)于對照組及實驗性糖尿病大鼠腸系膜微血管，觀察SNP對大鼠腸系膜微動、靜脈血管反應(yīng)性的影響；L-NAME孵育1min后，SNP對大鼠腸系膜微動、靜脈血管舒張作用的影響。 3.第三部分，局部滴加不同濃度酒精（0.025%～3.2%）及灌胃不同濃度酒精（0.375％～48％）對大鼠腸系膜微動、靜脈血管反應(yīng)性的影響。 結(jié)果： 1、第一部分，NE(10-12～10-4mol/L)濃度依賴性收縮大鼠腸系膜微動、靜脈直徑。NE誘導(dǎo)微動、靜脈收縮的濃度-效應(yīng)曲線的EC50（收縮達最大收縮50%時的藥物濃度）分別為(4.14±0.35)×10-9mol/L、(2.95±0.48)×10-8mol/L。與對照組相比較，實驗性糖尿病大鼠對NE的反應(yīng)性顯著減弱（P 0.05），NE誘導(dǎo)微動、靜脈收縮EC50分別為(5.39±0.29)×10-8mol/L、(4.78±0.39)×10-8mol/L。酚妥拉明孵育后，NE對對照組大鼠腸系膜微血管的收縮作用明顯強于STZ組。 2、第二部分，SNP(10-10～10-4mol/L)濃度依賴性舒張大鼠腸系膜微動、靜脈直徑，SNP誘導(dǎo)微動、靜脈舒張的EC50分別為（2.44±0.32）×10-8mol/L、(5.85±0.40)×10-8mol/L。與對照組相比較，，實驗性糖尿病大鼠腸系膜微血管對SNP的反應(yīng)性明顯減弱（P 0.05），SNP誘導(dǎo)微動、靜脈舒張EC50分別為(6.66±0.43)×10-8mol/L、(8.78±0.59)×10-8mol/L。L-NAME孵育后，SNP對對照組大鼠腸系膜微血管的舒張率明顯高于STZ組。 3、第三部分，酒精濃度依賴性舒張大鼠腸系膜微動、靜脈直徑（P 0.05），局部滴加酒精誘導(dǎo)微動、靜脈舒張EC50分別為（0.13±0.02）%、（0.17±0.02）%，灌胃酒精誘導(dǎo)微動、靜脈舒張的EC50分別為（2.81±0.22）%、（2.85±0.21）%。與對照組相比較，局部滴加酒精誘導(dǎo)微動、靜脈舒張的EC50分別為(0.25±0.03)%、（0.32±0.03）%，灌胃酒精誘導(dǎo)微動、靜脈舒張的濃度-效應(yīng)曲線的EC50分別為(2.79±0.23)%、（3.25±0.23）%，實驗性糖尿病大鼠腸系膜微血管對酒精的反應(yīng)性明顯減弱。 結(jié)論： 1. STZ誘導(dǎo)實驗性糖尿病大鼠腸系膜微循環(huán)對NE的反應(yīng)性顯著減弱。2.STZ誘導(dǎo)實驗性糖尿病大鼠腸系膜微循環(huán)對SNP的反應(yīng)性明顯減弱。3. STZ誘導(dǎo)實驗性糖尿病大鼠腸系膜微循環(huán)對酒精的反應(yīng)性明顯減弱。
[Abstract]:Objective: To study the effect of Norepinephrine (NE), sodium nitroprusside (Sodium nitroprusside, SNP) and alcohol (Alcohol) on the mesenteric micromovement and venous reactivity of the experimental diabetic rats.
Methods: 78 normal male SD rats (220 ~ 250g) were used to induce 78 rats of experimental diabetic rats (STZ). The microcirculation images of mesenteric microcirculation in anesthetized rats were collected by BI-2000 medical image analysis system. The different concentrations of NE (10-12 ~ 10-4mol/L), SNP (10-10 to 10-4mol), alcohol (0.025% to 3.2%) and different concentration of alcohol in the gavage were observed. The effect of sperm (0.375% ~ 48%, 5min) on the mesenteric microvascular diameter of experimental diabetic rats and the effect of phentolamine (Phentolamine) on the vasoconstrictor effect of NE and the effect of L-nitroarginine methyl ester (L-NAME) on the vasodilatation of SNP were observed.
1. in the first part, the mesenteric microvessels of the control group and experimental diabetic rats were partially added with different concentrations of NE (10-12 ~ 10-4mol/L) in the control group and experimental diabetic rats. The effects of NE on the mesenteric micromovement and venous reactivity of the rats were observed. After the incubation of phentolamine for 1min, the effect of NE on the mesenteric micromovement and venous vasoconstriction in rats.
2. in the second part, the mesenteric microvessels of the control group and experimental diabetic rats were separately added with different concentrations of SNP (10-10 ~ 10-4mol/L) in the control group and experimental diabetic rats. The effects of SNP on the mesenteric micromovement and venous reactivity of the rats were observed. After L-NAME incubation, the effect of SNP on the mesenteric micromovement and the vasodilatation of the rats was induced by SNP.
3. in the third part, the effects of different concentrations of alcohol (0.025% ~ 3.2%) and alcohol (0.375% ~ 48%) on the reactivity of the mesentery and venous reactivity of the rat mesentery (0.375% ~ 48%) were added.
Result:
1, the first part, NE (10-12 ~ 10-4mol/L) concentration dependent contraction rats mesenteric micromovement, venous diameter.NE induced fretting, the concentration effect curve of venous contraction of EC50 (the maximum contraction of 50% when the contraction of 50%) was (4.14 + 0.35) x 10-9mol/L, (2.95 + 0.48) x 10-8mol/L. compared with the control group, experimental diabetic rats N The reactivity of E was significantly decreased (P 0.05), NE induced micromovement and venous systolic EC50 was (5.39 + 0.29) x 10-8mol/L respectively, and (4.78 + 0.39) x 10-8mol/L. phentolamine incubated by NE. The contractile effect of NE on the mesenteric microvessels of the control group was significantly stronger than that of the STZ group.
The 2, second part, SNP (10-10 ~ 10-4mol/L) concentration dependent diastolic rat mesenteric micromovement, venous diameter, SNP induced micromovement, the diastolic EC50 was (2.44 + 0.32) x 10-8mol/L and (5.85 + 0.40) x 10-8mol/L. compared with the control group. The reactivity of mesenteric microvessels to SNP in experimental diabetic rats decreased significantly (P 0.05), SNP induction The diastolic diastolic rate of EC50 was (6.66 + 0.43) x 10-8mol/L and (8.78 + 0.59) x 10-8mol/L.L-NAME respectively. The diastolic rate of the mesenteric microvessel in the control group was significantly higher than that of the STZ group.
The 3 and third parts, the alcohol concentration dependent diastolic rat mesenteric micromovement, the diameter of the vein (P 0.05), the local dripping of alcohol induced micromovement, and the diastolic EC50 were (0.13 + 0.02)%, (0.17 + 0.02)%, the gavage alcohol induced micromovement, the EC50 of the diastolic diastole was (2.81 + 0.22)%, and (2.85 + 0.21)% respectively. Compared with the control group, the local drop of alcohol induced micromanipulation was compared with the control group. The diastolic EC50 was (0.25 + 0.03)% and (0.32 + 0.03)% respectively. The alcohol induced micromovement was induced by intragastric alcohol, and the EC50 of the concentration effect curve of venous diastole was (2.79 + 0.23)% and (3.25 + 0.23)% respectively. The reactivity of mesenteric microvessels to alcohol in experimental diabetic rats decreased significantly.
Conclusion:
1. STZ induced diabetic rat mesenteric microcirculation significantly reduced the reactivity of mesenteric microcirculation to NE induced by.2.STZ induced diabetic rats, the reactivity of mesenteric microcirculation to SNP was significantly reduced by.3. STZ induced diabetic rats, and the reactivity of mesenteric microcirculation to alcohol was significantly weakened in experimental diabetic rats.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R587.1

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