本文選題：下丘神經(jīng)元 + on-BF頻率�。� 參考：《華中師范大學(xué)》2014年碩士論文

【摘要】：頻率是聲信號(hào)的參數(shù)之一,它在傳遞聲音信息方面起著重要作用,頻率調(diào)諧(frequency tuning)成為聽(tīng)覺(jué)神經(jīng)元的一種重要功能。通過(guò)比較外周和中樞聽(tīng)覺(jué)神經(jīng)元頻率調(diào)諧曲線(frequency tuning curve, FTC)的形狀,發(fā)現(xiàn)中樞聽(tīng)覺(jué)神經(jīng)元具有更強(qiáng)的對(duì)頻率的調(diào)諧和分析的能力。由于缺乏來(lái)自興奮性和抑制性突觸輸入之間相互整合在銳化頻率調(diào)諧方面的作用的直接實(shí)驗(yàn)證據(jù),使得銳化頻率調(diào)諧的機(jī)制仍不完全清楚。因而,我們?cè)诒狙芯恐胁捎迷隗w細(xì)胞內(nèi)記錄的方法,探討了由on-BF (on best frequency)和偏離BF,即off-BF (off best frequency)頻率激活的抑制在銳化小鼠下丘(inferior colliculus, IC)神經(jīng)元頻率調(diào)諧方面的作用。 從31只由戊巴比妥鈉(Nembutal,0.02mL/g b.wt.)麻醉的成年健康的昆明小鼠(Mus musculus, Km) IC上總共獲得了87個(gè)聲敏感神經(jīng)元,這些神經(jīng)元的記錄深度(μm)和最佳頻率(kHz)的范圍分別為633-1950(1368.4±364.1)、7-47(22.3±10.9)。通過(guò)對(duì)其中53個(gè)神經(jīng)元的頻率調(diào)諧進(jìn)行分析,它們可以被分成2種類型,一種類型是在興奮性頻率調(diào)諧曲線(excitatory frequency tuning curves, EFTC)低頻邊或/和高頻邊帶有側(cè)抑制區(qū)(lateral inhibitory area, LIA)的神經(jīng)元(n=25),另一種類型則是在EFTC的高、低頻邊均不具有LIA的神經(jīng)元(n=28)。在雙聲刺激條件下,由on-BF和off-BF所激活的抑制均能銳化這些神經(jīng)元的FTC。另外,對(duì)于28個(gè)不具有LIA的神經(jīng)元,它們頻率調(diào)諧的銳化主要依賴于on-BF頻率激活的抑制,或許還有來(lái)自IC下某個(gè)聽(tīng)覺(jué)中樞的側(cè)抑制(lateral inhibition, LI)的參與。 通過(guò)比較由on-BF和off-BF所激活的兩種抑制銳化頻率調(diào)諧的程度,統(tǒng)計(jì)學(xué)分析發(fā)現(xiàn)兩種抑制在銳化的程度上存在有顯著的差別(p0.05)。這些結(jié)果提示由on-BF和off-BF頻率所激活的抑制性突觸輸入共同銳化下丘神經(jīng)元的頻率調(diào)諧。
[Abstract]:Frequency is one of the parameters of acoustic signal, which plays an important role in transmitting sound information. Frequency tuning (frequency tuning) becomes an important function of auditory neurons. By comparing the shape of frequency tuning curves of peripheral and central auditory neurons, it is found that central auditory neurons have stronger ability of frequency tuning and analysis. Due to the lack of direct experimental evidence from the integration of excitatory and inhibitory synaptic inputs in sharpening frequency tuning, the mechanism of sharpening frequency tuning is still unclear. In this study, we investigated the effects of on-BF (on best frequency) and off-BF (off best frequency) frequency activation on the frequency tuning of (inferior colliculus, IC neurons in sharpened mouse inferior colliculus by means of somatic recording. Nembutala (0.02mL / g b 路wt.) A total of 87 acoustic-sensitive neurons were obtained in anesthetized adult healthy Kunming mice (Ms) IC. The recording depth (渭 m) and optimal frequency (kHz) of these neurons ranged from 633-1950 (1368.4 鹵364.1) to 47 (22.3 鹵10.9), respectively. By analyzing the frequency tuning of 53 of these neurons, they can be divided into two types. One type is the neurons with LIA-25 on the low-frequency or / and high-frequency edge of the excitatory frequency tuning curve (excitatory frequency tuning curves,), and the other is the neuron with no LIA-LIA on the high side of the excitatory frequency tuning curve (excitatory frequency tuning curves,). The inhibition activated by on-BF and off-BF can sharpen the FTC of these neurons under the condition of double acoustic stimulation. In addition, for 28 neurons without LIA, the sharpening of their frequency tuning mainly depends on the inhibition of on-BF frequency activation, and maybe the lateral inhibition of (lateral inhibition, Li from an auditory center under IC. By comparing the tuning degree of sharpening frequencies activated by on-BF and off-BF, it was found that there was a significant difference in sharpening degree between the two kinds of inhibition (p0.05). These results suggest that inhibitory synaptic inputs activated by on-BF and off-BF frequencies jointly sharpen the frequency tuning of inferior colliculus neurons.
【學(xué)位授予單位】：華中師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：Q421

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 李安安,任雪萍,吳飛健,劉雙喜,王景玉,陳其才;普通伏翼蝠下丘神經(jīng)元基本聲反應(yīng)特性[J];動(dòng)物學(xué)雜志;2005年04期

2 付子英;唐佳;JEN Philip Hung-Sun;陳其才;;大蹄蝠回聲定位信號(hào)特征與下丘神經(jīng)元頻率調(diào)諧[J];動(dòng)物學(xué)雜志;2009年01期

3 陳其才,PhilipH.S.JEN,吳飛健;銳化蝙蝠聽(tīng)皮層神經(jīng)元頻率調(diào)諧的柱特征[J];動(dòng)物學(xué)研究;2001年03期

4 唐佳,皮建輝,王丹,吳飛健,陳其才;弱噪聲對(duì)小鼠下丘神經(jīng)元頻率調(diào)諧的影響(英文)[J];動(dòng)物學(xué)研究;2004年03期

5 ;Latency represents sound frequency in mouse IC[J];Science in China(Series C:Life Sciences);2007年02期

6 ;Topography of acoustic response characteristics in the midbrain inferior colliculus of Kunming mouse[J];Chinese Science Bulletin;2003年02期

7 白靜;唐佳;;聽(tīng)神經(jīng)元的頻率調(diào)諧[J];生物學(xué)雜志;2011年02期

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本文編號(hào)：2116083