本文選題：中樞疲勞 + 運動性疲勞　； 參考：《山東體育學院學報》2015年02期

【摘要】：目的:探討大鼠海馬組織膠質細胞源性神經(jīng)營養(yǎng)因子(glial cell line-derived neurotrophic factor,GDNF)、GDNF家族受體α-1信使RNA(GDNF?family receptorα-1mRNA,GFRα-1mRNA)及其蛋白表達在中樞疲勞后恢復期不同時相的動態(tài)變化特征。方法:雄性2月齡SD大鼠32只,隨機分為對照組(C組,n=8)、實驗組(E組,n=24);E組進行7周疲勞模型運動后,又隨機分為運動后即刻組(E0組,n=8)、恢復期12 h組(E1組,n=8)、恢復期24 h組(E2組,n=8)。C組正�；\養(yǎng),E組進行7周遞增負荷跑臺訓練。經(jīng)7周疲勞模型運動后即刻,C組和E0組腹腔注射10%水合氯醛糖漿(0.3m L/100g)麻醉后取海馬組織,用于測定5-羥色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、GDNF、GFRα-1mRNA及其蛋白表達;E1組和E2組分別在恢復期12 h、24 h做上述同樣取材和測試。結果:1)大鼠經(jīng)7周遞增負荷跑臺訓練后即刻,海馬組織5-HT升高(P0.01),DA下降(P0.01),DA/5-HT下降(P0.05)。2)運動后即刻海馬組織GDNF、GFRα-1mRNA相對表達率和GDNF、GFRα-1平均蛋白表達水平升高;恢復期12 h高于C組和E0組(P0.01);恢復期24 h降低并低于E1組(P0.01)。結論:1)經(jīng)7周疲勞模型運動后大鼠已經(jīng)出現(xiàn)中樞疲勞。2)運動性中樞疲勞后大鼠海馬組織GDNF、GFRα-1mRNA及其蛋白表達水平上升,提示GDNF和GFRα-1可能參與了中樞疲勞恢復的神經(jīng)生物學調控過程。
[Abstract]:Aim: to investigate the dynamic changes of GDNF family receptor 偽 -1mRNA-GFR 偽 -1mRNA and its protein expression in the recovery phase after central fatigue in rat hippocampal tissue with glial cell-derived neurotrophic factor (glial cell line-derived neurotrophic factor-GDNF). Methods: Thirty-two male 2-month-old SD rats were randomly divided into control group (group C, n = 8) and experimental group (group E, n = 24) after 7 weeks of fatigue exercise. They were randomly divided into three groups: immediate exercise group (E0 group), 12 h recovery group (E1 group) and 24 h recovery period group (E2 group). Group C was given 7 weeks incremental load treadmill training. After 7 weeks of fatigue exercise, hippocampal tissue was taken out in group C and group E0 by intraperitoneal injection of 10% chloral hydrate syrup (0.3m L / 100g). To determine the expression of 5-hydroxytryptamine-5-HT, dopamine (DA), GDNF-GFR 偽 -1 mRNA and their protein expression in E1 group and E2 group, the same materials were obtained and tested at 12 h and 24 h after recovery, respectively. Results (1) immediately after 7 weeks of increasing load treadmill training, the hippocampal 5-HT increased (P0.01) DA decreased (P0.01) and DA-5-HT decreased (P0.05). Immediately after exercise, the relative expression rate of GDNFGFR 偽 -1 mRNA and the average expression level of GDNFGFR 偽 -1 protein in hippocampal tissue were increased. The recovery period was 12 h higher than that of C group and E0 group (P0.01), and 24 h of convalescence period was lower than that of E1 group (P0.01). Conclusion (1) after 7 weeks of exercise, the rats have developed central fatigue. 2) after exercise induced fatigue, the expression of GDNFF GFR 偽 -1 mRNA and its protein in hippocampal tissue of rats increased, suggesting that GDNF and GFR 偽 -1 may be involved in the neurobiological regulation of central fatigue recovery.
【作者單位】： 山東體育學院;山東省微山縣實驗中學;山東省體育科學研究中心;
【基金】：山東省自然科學基金(ZR2009CQ032)
【分類號】：G804.7

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【共引文獻】

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1 胡柏平;，

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