發(fā)布時間：2018-07-08 10:12

  本文選題：亞低溫 + 缺氧缺血性腦損傷��； 參考：《山西醫(yī)科大學》2014年碩士論文

【摘要】：目的利用亞低溫干預(yù)新生大鼠缺氧缺血性腦損傷（hypoxic-ischemic braindamage, HIBD）動物模型，觀察亞低溫干預(yù)缺氧缺血性腦損傷后腦組織在不同時間點NF-B基因的表達含量，探討亞低溫干預(yù)HIBD后NF-B基因表達的變化，為亞低溫治療缺氧缺血性腦病提供理論依據(jù)和新的治療途徑。 方法7日齡SD大鼠120只，雌雄不限，體重12~18g，隨機分為A組（假手術(shù)組），B組（常溫組），C組（亞低溫組），每組根據(jù)處死時間不同又分為5個亞組：1h組，6h組，12h組，24h組，48h組各8只，A組乙醚吸入麻醉后切開頸部皮膚，分離但不結(jié)扎一側(cè)頸總動脈；B組乙醚吸入麻醉后切開頸部皮膚，分離結(jié)扎一側(cè)頸總動脈后置于缺氧倉中（含8%氧氣）2h；C組乙醚麻醉后切開頸部皮膚，分離結(jié)扎一側(cè)頸總動脈并置于缺氧倉（含8%氧氣）2h，并在缺血缺氧后即刻給予亞低溫干預(yù)。利用RT-PCR技術(shù)在mRNA水平檢測各組新生大鼠腦組織各組時間點的NF-κB基因含量。 結(jié)果（1）HIBD后新生大鼠均表現(xiàn)出不同程度行為異常改變。（2）B組不同時間點腦標本，外觀形態(tài)均表現(xiàn)出不同程度的水腫、萎縮，甚至可出現(xiàn)液化壞死。亞低溫組干預(yù)缺氧缺血性腦損后不同時間點腦標本的損傷程度較常溫組減輕，僅出現(xiàn)輕度水腫。（3）假手術(shù)組腦組織中NF-κBmRNA表達量甚微，常溫組隨著時間的延長，NF-κBmRNA表達逐漸增加，24h達到高峰，，48h后仍高于假手術(shù)組，亞低溫組各時間點NF-κBmRNA含量均低于常溫組（p＜0.01），24h組下降最為顯著，亞低溫組和常溫組各時間點NF-κBmRNA含量與假手術(shù)組比較，差異有統(tǒng)計學意義（p＜0.01）。 結(jié)論（1）亞低溫干預(yù)的新生大鼠缺氧缺血性腦損傷的腦組織標本損害的病理改變程度減輕，說明亞低溫治療對新生大鼠缺氧缺血性腦損傷的腦組織起到了保護作用。(2)常溫組腦組織中，NF-B的含量隨著時間的延長逐漸增多，24h達到高峰，48h仍明顯高于假手術(shù)組，表明NF-B參與缺氧缺血性腦損傷的全過程并起著重要的作用，而在亞低溫干預(yù)后的腦組織中，NF-B的含量明顯降低，24h下降最為顯著，此結(jié)果表明亞低溫的治療作用可能通過抑制NF-κB的基因表達來起到神經(jīng)保護的作用。
[Abstract]:Objective to observe the expression of NF-B gene in brain tissue of neonatal rats after mild hypothermia treatment with hypoxic-ischemic brain damage (HIBD) at different time points. To explore the changes of NF-B gene expression after mild hypothermia intervention in HIBD, and to provide a theoretical basis and a new therapeutic approach for mild hypothermia treatment of hypoxic-ischemic encephalopathy. Methods 120 SD rats of 7 days old, male and female, were used. The rats were randomly divided into two groups: group A (sham operation group), group B (normal temperature group) and group C (mild hypothermia group). Each group was divided into 5 subgroups according to the time of death. Each group was divided into 5 subgroups: 1: 1 h group, 12 h group, 24 h group, 48 h group, 8 rats in group A, 8 rats in group A after inhaled anesthesia. One side of common carotid artery was seperated but not ligated. In group B, the neck skin was cut by ether inhalation anesthesia. The common carotid artery was seperated and ligated and placed in anoxic chamber (containing 8% oxygen) for 2 h. The common carotid artery was isolated and ligated and placed in anoxic chamber (containing 8% oxygen) for 2 h and then treated with mild hypothermia immediately after ischemia and hypoxia. The content of NF- 魏 B gene in brain tissue of neonatal rats at different time points was detected by RT-PCR at mRNA level. Results (1) the neonatal rats after HIBD showed different degrees of abnormal behavior. (2) in group B, brain morphology showed edema, atrophy, and even liquefaction and necrosis at different time points. The degree of brain injury in mild hypothermia group was less than that in normothermic group at different time points after hypoxic-ischemic brain injury. (3) the expression of NF- 魏 B mRNA in brain tissue of sham operation group was very small. The expression of NF- 魏 B mRNA in normothermic group was increased gradually with time, and the expression of NF- 魏 B mRNA was still higher than that in sham operation group after 24 hours. The content of NF- 魏 B mRNA in mild hypothermia group was lower than that in normothermic group (p < 0.01). The content of NF- 魏 B mRNA in mild hypothermia group and normal temperature group was significantly higher than that in sham operation group (p < 0.01). Conclusion (1) the pathological changes of brain tissue in neonatal rats treated with mild hypothermia were alleviated after hypoxic-ischemic brain injury. The results showed that mild hypothermia therapy could protect the brain tissue from hypoxic-ischemic brain injury in neonatal rats. (2) the content of NF-B in brain tissue of normothermic group gradually increased with time and reached the peak at 24h and 48h, which was still significantly higher than that in sham operation group. The results indicated that NF-B was involved in the whole process of hypoxic-ischemic brain injury and played an important role, but the content of NF-B in brain tissue decreased significantly after mild hypothermia intervention for 24 hours. These results suggest that mild hypothermia may play a neuroprotective role by inhibiting NF- 魏 B gene expression.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R743.31

【相似文獻】

相關(guān)期刊論文 前10條

1 李奮強,羅毅,黃文龍,李松年,韋剛;應(yīng)用亞低溫治療原發(fā)性腦干損傷10例報告[J];右江民族醫(yī)學院學報;2000年01期

2 張鯤鵬,季曉俠;亞低溫治療重型顱腦損傷病人的護理對策[J];臨床護理雜志;2003年01期

3 蘇萬東,吉訓明,吳浩,凌鋒;亞低溫對局灶性腦缺血治療時間窗的實驗研究進展[J];中國腦血管病雜志;2005年09期

4 江基堯,朱誠,盧亦成,張光霽,于明琨,陳左權(quán),梁玉敏,高國一,文淑華,俞美定,董萍;亞低溫治療重型顱腦傷患者的臨床療效(論著摘要)[J];中華創(chuàng)傷雜志;1997年01期

5 鄧其峻,吳敏,王國福;亞低溫治療急性重型顱腦損傷的臨床研究[J];中國急救醫(yī)學;2000年07期

6 洪素風,蔡淑萍;重型顱腦損傷急性期亞低溫治療的護理[J];護理學雜志;2001年12期

7 王惠芬,何曉華;以顱腦損傷為主多發(fā)傷病人亞低溫治療的護理[J];中華急診醫(yī)學雜志;2001年06期

8 袁遠;亞低溫治療腦損傷并發(fā)肺部感染的原因分析及護理對策[J];現(xiàn)代護理;2002年06期

9 陳漢民,張誠華,廖圣芳;重型顱腦損傷中止亞低溫治療原因分析[J];中國臨床神經(jīng)外科雜志;2003年03期

10 尹波,許t

本文編號：2107094