本文選題：芫花根醇提顆粒 + 甲基潑尼松龍�。� 參考：《中國中西醫(yī)結(jié)合雜志》2015年08期

【摘要】：目的研究芫花根醇提顆粒(金腰帶)聯(lián)合甲基潑尼松龍對脊髓損傷(spinal cordinjury,SCI)大鼠行為學(xué)、腦源性神經(jīng)營養(yǎng)因子(brain derived neurotrophic factor,BDNF)表達(dá)及N-甲基-D-門冬氨酸(N-methyl-D-aspartate,NMDA)水平的影響。方法 SD成年雄性大鼠,隨機分為假手術(shù)組、脊髓挫傷組(模型組)、金腰帶組[灌胃金腰帶50 mg/(kg·d),共5天]、甲基潑尼松龍組(脊髓鈍挫傷術(shù)后8 h內(nèi)肌肉注射50 mg/kg甲基潑尼松龍,此后甲基潑尼松龍劑量每天減少10 mg/kg,共5天)及聯(lián)合組(金腰帶和甲基潑尼松龍聯(lián)合干預(yù))。各組大鼠均在術(shù)后(0、3、7、28天)進(jìn)行后肢行為學(xué)評價[神經(jīng)行為學(xué)(Basso Beattie and Bresnahan,BBB)評分]。術(shù)后13天,每組取8只大鼠,麻醉后取損傷脊髓T_(8-10)液氮中保存,采用[~3H]MK-801放射性配基分析法測定NMDA受體親和力(Kd)和最大結(jié)合數(shù)量(Bmax);取術(shù)后28天大鼠損傷脊髓,采用免疫組織化學(xué)法檢測BDNF表達(dá)。結(jié)果與假手術(shù)組比較,模型組腹角和背角BDNF陽性神經(jīng)元數(shù)量升高,Bmax(470±34)升高,Kd降低,術(shù)后3~28天BBB評分降低,差異有統(tǒng)計學(xué)意義(P0.05)。與模型組比較,各給藥組BDNF陽性神經(jīng)元數(shù)量及Kd升高,術(shù)后3~28天BBB評分升高(P0.05),Bmax[甲基潑尼松龍組:660±15,金腰帶組:646±25,聯(lián)合組:510±21]降低(P0.05)。與甲基潑尼松龍組比較,金腰帶組及聯(lián)合組BDNF陽性神經(jīng)元數(shù)量及Kd升高(P0.05),術(shù)后7~28天BBB評分升高(P0.05),Bmax降低(P0.05)。與金腰帶組比較,聯(lián)合組BDNF陽性神經(jīng)元數(shù)量及Kd升高(P0.05),Bmax降低(P0.05)。結(jié)論金腰帶能有效改善SCI大鼠后肢運動功能,增加脊髓組織中BDNF的表達(dá),通過影響脊髓NMDA受體減輕脊髓繼發(fā)性損傷,對SCI有一定的治療與保護(hù)作用,效果優(yōu)于甲基潑尼松龍且與其有協(xié)同作用。
[Abstract]:Objective to study the effects of Daphne genkwa root alcohol extract granule (gold belt) combined with methylprednisolone on behavior, brain derived neurotrophic factor (BDNF) expression and N-methyl-Daspartate N-methyl-D-aspartate (NMDAs) level in spinal cord injury (sci) rats. Methods Sprague-Dawley adult male rats were randomly divided into sham operation group, spinal cord contusion group (model group), golden belt group [intragastric infusion of 50 mg/(kg belt for 5 days] and methylprednisolone group (50 mg/kg methylprednisolone intramuscularly injected within 8 hours after spinal cord contusion). After that, the dosage of methylprednisolone was reduced by 10 mg / kg per day for 5 days) and combined group (combined with gold belt and methylprednisolone). All the rats in each group were evaluated for the behavior of the hind limbs on the 28th day after operation. [the neurobehavioral score was Basso Beattie and Bresnahan BBB]. On the 13th day after operation, 8 rats in each group were taken from each group. The injured spinal cord was preserved in liquid nitrogen after anesthesia. The affinity of NMDA receptor (KdD) and the maximum binding amount (Bmax1) were determined by [3H] MK-801 radioligand assay, and the injured spinal cord was harvested 28 days after operation. The expression of BDNF was detected by immunohistochemistry. Results compared with the sham-operated group, the number of BDNF positive neurons in the ventral horn and dorsal horn of the model group was increased and the number of BDNF positive neurons in the ventral horn and dorsal horn of the model group was increased. The number of BDNF positive neurons in the model group was significantly higher than that in the sham operation group (P < 0.05). Compared with the model group, the number of BDNF positive neurons and KD increased in each administration group, and the BBB score increased 328 days after operation [methylprednisolone group: 660 鹵15, gold belt group: 646 鹵25, combined group: 510 鹵21] decreased P0.05. Compared with methylprednisolone group, the number of BDNF positive neurons and KD in gold belt group and combined group were increased (P 0.05). Compared with the gold belt group, the number of BDNF positive neurons and the increase of KD in the combined group decreased P0.05B _ (max). Conclusion Gold belt can effectively improve the motor function of hind limbs and increase the expression of BDNF in spinal cord tissue of sci rats. It can reduce the secondary injury of spinal cord by affecting NMDA receptor of spinal cord and has a certain therapeutic and protective effect on sci. The effect was superior to and synergistic with methylprednisone.
【作者單位】： 云南省玉溪市中醫(yī)醫(yī)院;昆明醫(yī)學(xué)院神經(jīng)科學(xué)研究所;云南省昆明市第一人民醫(yī)院神經(jīng)外科;
【基金】：玉溪市科技合作專項計劃項目
【分類號】：R285.5

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