本文選題：認(rèn)知障礙 + 應(yīng)激免疫反應(yīng)　； 參考：《山東中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：通過麻醉行脾切除術(shù)建立POCD大鼠模型，探討POCD的發(fā)病機(jī)制，以及評(píng)價(jià)術(shù)后應(yīng)用四逆湯與多奈哌齊對(duì)POCD的療效。 方法：選取符合實(shí)驗(yàn)要求的健康老齡雄性SD大鼠48只，體重550-650g，隨機(jī)分為3組，為四逆湯組(A組)、多奈哌齊組(B組)和對(duì)照組(C組)。術(shù)前連續(xù)六天進(jìn)行水迷宮訓(xùn)練，曠場試驗(yàn)。在相同的條件下用異氟醚麻醉行脾切除術(shù)。麻醉過程持續(xù)4h。術(shù)后待大鼠清醒后給予相應(yīng)的藥物灌胃，術(shù)后注意保溫。術(shù)后連續(xù)灌胃七天。術(shù)后1d行水迷宮及曠場試驗(yàn)，于術(shù)前1天、術(shù)后即刻、術(shù)后24h靜脈取血。術(shù)后3，7，21d只行水迷宮及曠場試驗(yàn)。最后取腦并切片，用免疫組化法檢測β-AP的表達(dá)。抽取的血液進(jìn)行ACTH、ALD、TNF-α水平及NSE、S-100β的含量測定。 結(jié)果：手術(shù)過程中大鼠生命體征平穩(wěn)，水迷宮實(shí)驗(yàn)結(jié)果表明，手術(shù)結(jié)束后第1、3天，A組大鼠逃避潛伏期明顯短于B組和C組(P0.01)。曠場實(shí)驗(yàn)結(jié)果：術(shù)后1d，A組的跨格總數(shù)、站立次數(shù)、排便次數(shù)與另兩組相比具有明顯差異，均具有統(tǒng)計(jì)學(xué)意義。而對(duì)于TNF-α，ACTH，ALD，NSE，S100β指標(biāo)，三組較術(shù)前均有增加，C組較A、B組的增加更明顯；β-AP的沉積亦如此。 結(jié)論：結(jié)果術(shù)后應(yīng)用四逆湯可有效抑制術(shù)后應(yīng)激反應(yīng)的發(fā)生，減少大腦中β-淀粉樣蛋白的沉積；降低術(shù)后認(rèn)知障礙發(fā)生率；術(shù)后應(yīng)用多奈哌齊可增加大鼠腦海馬區(qū)膽堿乙酰轉(zhuǎn)移酶及煙堿型乙酰膽堿受體的表達(dá)，，亦可減少大腦中β一淀粉樣蛋白的沉積，對(duì)術(shù)后認(rèn)知功能障礙可起到一定的防治作用。
[Abstract]:Objective: to establish a rat model of POCD by splenectomy under anesthesia, to explore the pathogenesis of POCD and to evaluate the effect of Sini decoction and Donepezil on POCD after operation. Methods: 48 male Sprague-Dawley (SD) rats, weighing 550-650 g, were randomly divided into three groups: Sini decoction group (group A), Donepezil group (group B) and control group (group C). Water maze training and open field test were performed for 6 days before operation. Splenectomy was performed with isoflurane anesthesia under the same conditions. The anesthesia lasted for 4 hours. After operation, the rats were given the appropriate medicine after waking up, and the heat preservation was noticed after operation. Postoperative gastric perfusion for 7 days. Water maze test and open field test were performed 1 day after operation, 1 day before operation, immediately after operation and 24 hours after operation. Water labyrinth and open field test were performed only 3 days and 7 days after operation. Finally, the expression of 尾-AP was detected by immunohistochemical method. The level of TNF- 偽 and the content of NSES-100 尾 in ACTHG ALDN were determined. Results: the vital signs of rats were stable during the operation. The results of water maze test showed that the escape latency of rats in group A was significantly shorter than that in group B and group C on the 1st day after operation. The results of open field experiment showed that there were significant differences in the total number of translattice, standing times and defecation times between group A and the other two groups on the 1st day after operation, all of which were statistically significant. But for TNF- 偽 ACTHN, NSES100 尾, the increase of S100 尾 in all three groups was more obvious than that in group A B, and so was the deposition of 尾 -AP. Conclusion: the post-operative application of Sini decoction can effectively inhibit the occurrence of post-operative stress response, reduce the accumulation of 尾 -amyloid protein in the brain, and reduce the incidence of postoperative cognitive impairment. The expression of choline acetyltransferase and nicotinic acetylcholine receptor and 尾 -amyloid protein deposition in the brain of rats were increased after operation with Donepezil, which may play a role in the prevention and treatment of postoperative cognitive dysfunction.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R619

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 吳文春;董敏;曹雅軍;;老年患者圍手術(shù)期NSE、S-100β蛋白水平變化與POCD的關(guān)系[J];臨床醫(yī)學(xué)工程;2012年10期

本文編號(hào)：2035126