本文選題：靈芝孢子粉 + 大鼠腎臟��； 參考：《遵義醫(yī)學(xué)院學(xué)報》2016年06期

【摘要】：目的研究靈芝孢子粉(GLS)對亞急性鎘中毒大鼠腎臟的保護作用。方法雄性SD大鼠50只,按體重分層后,采用隨機數(shù)字法分為5組,每組10只,即正常對照組(C組)、單獨染鎘組(Cd組)和3個給藥組(0.1、0.5、1.0 g/kg GLS+CdCl_2,即GCd_L組、GCd_M組、GCd_H組)。除C組外,其它組大鼠每天灌胃CdCl_2(6.5mg/kg),3個給藥組每天灌胃靈芝孢子粉懸濁液,C組和Cd組灌等體積生理鹽水,持續(xù)42d,定期稱重并觀察動物的一般表現(xiàn)。染毒結(jié)束后麻醉、取血、取腎臟;血生化檢測腎功能相關(guān)指標(biāo);原子吸收光譜法(AAS)檢測腎組織及其線粒體、微粒體等亞細胞器中鎘的含量;腎臟病理HE染色切片觀察病理組織學(xué)改變。結(jié)果與C組比較Cd組肌酐、尿素、尿酸、胱抑素-c均有不同程度升高,給予GLS干預(yù)后,各項指標(biāo)有不同程度降低,GCd_M組、GCd_H組肌酐變化與Cd組比較,差異有統(tǒng)計學(xué)意義(P0.05);給予靈芝孢子粉干預(yù)后,鎘在各亞細胞器中有不同程度降低,差異有統(tǒng)計學(xué)意義(P0.05);HE染色顯示,單獨染鎘組腎組織細胞間質(zhì)充血嚴重,細胞排列雜亂,細胞邊界不清晰,腎小管管腔不明顯,核溶解情況嚴重,而給藥組以上情況均有不同程度改善,并且高劑量組效果優(yōu)于中劑量組。結(jié)論靈芝孢子粉對亞急性鎘中毒大鼠腎臟有保護作用。
[Abstract]:Objective to study the protective effect of Ganoderma lucidum spore powder (GLS) on the kidney of subacute cadmium poisoning rats. Methods 50 male SD rats were divided into 5 groups according to the weight stratification and 10 rats in each group, that is, the normal control group (group C), the cadmium group (group Cd) and the 3 administration groups (0.1,0.5,1.0 g/kg GLS+CdCl_2, GCd_L, GCd_M, GCd_H group). Outside the group, the rats in the other groups were intragastric CdCl_2 (6.5mg/kg) every day. 3 administration groups were filled with Ganoderma lucidum spore powder suspension daily, C group and Cd group were filled with physiological saline, continuous 42d, regular weighing and observation of the general performance of the animals. After the end of the poisoning, the blood was taken and the kidney was taken; the blood biochemical test of renal function related indexes; atomic absorption spectrometry (AAS) detection. The content of cadmium in renal tissue and its mitochondria, microsome and other subcellular organelles; pathological changes of kidney pathology HE staining section. Results compared with group C, the creatinine, urea, uric acid and cystatin -c were elevated in different degrees. The prognosis of GLS was given to the group of GLS, the indexes were reduced in varying degrees, GCd_M and GCd_H groups were compared with that of the Cd group. The difference was statistically significant (P0.05). The prognosis of Ganoderma lucidum spore powder decreased in varying degrees, and the difference was statistically significant (P0.05). HE staining showed that the renal interstitial cell congestion was serious, the cells were arranged and disorderly, the cell boundary was not clear, the renal tubules were not obvious, and the nuclear dissolution was serious. The effect of the high dose group was better than that of the middle dose group. Conclusion Ganoderma lucidum spore powder has protective effect on the kidney of subacute cadmium poisoning rats.
【作者單位】： 遵義醫(yī)學(xué)院公共衛(wèi)生學(xué)院毒理學(xué)教研室;
【基金】：貴州省優(yōu)秀科技教育人才省長專項基金資助項目(NO:黔科教[2009]04)
【分類號】：R285.5

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