本文選題：更年期綜合征 + 精烏膠囊�。� 參考：《北京中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：更年期綜合征(Climacteric syndrome, CS)是婦女絕經(jīng)前后由于性激素波動(dòng)或減少而引起的一系列以自主神經(jīng)系統(tǒng)功能紊亂為主,伴有神經(jīng)心理癥狀的一組癥候群(包括潮紅潮熱、煩躁易怒、失眠健忘、頭暈、頭疼、腰疼、骨質(zhì)疏松等)。卵巢功能衰退引起的雌激素水平降低,以及相伴隨的神經(jīng)內(nèi)分泌紊亂和植物神經(jīng)功能紊亂直接導(dǎo)致了更年期綜合征的發(fā)生。精烏膠囊主要由制何首烏、黃精(制)、女貞子(制)、墨旱蓮4味中藥組成,具有補(bǔ)肝腎、益精血、壯筋骨之功效,擬用于更年期綜合征的治療。 本研究探索了更年期綜合征動(dòng)物模型,建立了更接近更年期綜合征臨床癥狀的病-證結(jié)合動(dòng)物模型(去勢(shì)加腎虛),模擬了更年期綜合征的臨床表現(xiàn)：血液中下丘腦-垂體-性腺軸的相關(guān)激素發(fā)生改變,植物神經(jīng)功能的紊亂等。并在此模型上,觀察了精烏膠囊對(duì)更年期綜合征的改善作用,為該復(fù)方的臨床應(yīng)用提供實(shí)驗(yàn)依據(jù)。 1觀察去勢(shì)大鼠模型各指標(biāo)的變化及精烏膠囊的干預(yù)作用 目的觀察去勢(shì)(10周)模型大鼠各指標(biāo)的變化及精烏膠囊對(duì)其的影響。方法健康雌性,育齡未孕SD大鼠90只,隨機(jī)留取15只為正常對(duì)照組,其余大鼠均手術(shù)摘除雙側(cè)卵巢。自第6-10天,每日對(duì)大鼠進(jìn)行陰道涂片檢查,以不出現(xiàn)動(dòng)情期反應(yīng)為去勢(shì)成功。大鼠去勢(shì)6周后,隨機(jī)分為：去勢(shì)模型組、精烏膠囊1.62、0.81、0.405g/kg三個(gè)劑量組,坤寶丸組(0.823g/kg)、按照相應(yīng)劑量灌胃給藥,正常對(duì)照組及去勢(shì)模型組給于等量飲用水,連續(xù)4周。給藥期限結(jié)束后,各組動(dòng)物進(jìn)行行為學(xué)指標(biāo)觀察(敞箱活動(dòng)、自主活動(dòng)、對(duì)戊巴比妥鈉誘導(dǎo)睡眠時(shí)間的影響)及直腸溫度測(cè)定；動(dòng)物麻醉后取血,測(cè)全血粘度；部分血液離心取血清,測(cè)定血清孕酮(P)、雌二醇(E2)、黃體生成素(LH)、卵泡刺激素(FSH)、催乳素(PRL)、促性腺激素釋放激素(GnRH)含量；各組動(dòng)物處死取臟器(子宮、腎、腎上腺)稱重,計(jì)算臟器系數(shù)。結(jié)果去勢(shì)(10周)模型組大鼠血清相關(guān)激素的變化是：GnRH顯著下降(P0.01),P含量降低1/2(因標(biāo)準(zhǔn)差大,未見(jiàn)顯著性差異)；子宮系數(shù)顯著下降(P0.01),腎系數(shù)顯著下降(P0.05)；直腸溫度顯著下降(p0.01)；各切率(1s-1、30s-1、70s-1、100s-1、150s-1、200s-1)全血粘度顯著增大(P0.01)。其余指標(biāo)未見(jiàn)明顯變化。與去勢(shì)(10周)模型組比較,精烏膠囊在某些指標(biāo)上有逆轉(zhuǎn)作用,表現(xiàn)在各劑量大鼠直腸溫度回升(P0.05或P0.01),GnRH含量回升。但本次研究中去勢(shì)(10周)模型大鼠血清E2、FSH、LH、PRL水平的變化與文獻(xiàn)報(bào)道有差異,初步分析與指標(biāo)檢測(cè)時(shí)間點(diǎn)有關(guān),文獻(xiàn)中多在去勢(shì)后5-6周。結(jié)論由于本實(shí)驗(yàn)復(fù)制的去勢(shì)模型未顯示更年期綜合征典型臨床表現(xiàn),故不能用于評(píng)價(jià)精烏膠囊的作用,僅作為方法學(xué)的探索。 2去勢(shì)加腎虛類(lèi)更年期綜合征狀態(tài)模型的建立及精烏膠囊的干預(yù)作用 2.1去勢(shì)加腎虛大鼠類(lèi)更年期綜合征狀態(tài)的模型的建立 目的建立去勢(shì)加腎虛大鼠模型,以評(píng)價(jià)該模型的特征。方法健康雌性,育齡未孕SD大鼠50只,隨機(jī)留取15只作為正常對(duì)照組,其余大鼠均手術(shù)摘除雙側(cè)卵巢。大鼠去勢(shì)后,自第5-7天,每日對(duì)大鼠進(jìn)行陰道涂片檢查,將不出現(xiàn)動(dòng)情期反應(yīng)大鼠隨機(jī)分為單去勢(shì)組、去勢(shì)加腎虛組。去勢(shì)加腎虛組大鼠每日肌注氫化可的松(25mg/kg),連續(xù)兩周。每周測(cè)大鼠體重；于建模完成時(shí),測(cè)大鼠尾根、腋下、足底溫度；于建模完成時(shí)、建模4周、建模6周檢測(cè)大鼠血清相關(guān)激素(E2、FSH、LH、GnRH)水平；于建模完成前1周,建模完成時(shí),建模1周,建模2周,建模3周,建模5周,建模6周測(cè)大鼠直腸溫度；于建模6周后,依次觀察大鼠行為(敞箱活動(dòng)、自主活動(dòng)、戊巴比妥鈉誘導(dǎo)睡眠)以及臟器(子宮、腎、腎上腺)系數(shù)。結(jié)果與正常對(duì)照組比較,單去勢(shì)大鼠各指標(biāo)變化如下：建模完成時(shí),E2顯著下降(P0.01),FSH顯著升高(P0.05)；建模4周,GnRH顯著下降(P0.01)；建模6周,E2顯著下降(P0.05),LH顯著升高(P0.05),子宮系數(shù)、腎系數(shù)均顯著降低(P0.01),在敞箱中垂直活動(dòng)次數(shù)顯著升高(P0.05)；體重快速增加,直腸溫度略有下降。去勢(shì)加腎虛大鼠各指標(biāo)變化如下：建模完成時(shí),E2、GnRH均顯著下降(P0.01或P0.05),FSH顯著升高(P0.01),大鼠足底、腋下溫度均顯著升高(P0.05或P0.01)；建模4周,E2、GnRH顯著下降(P0.05或P0.01)；建模6周,E2顯著下降(P0.05),GnRH顯著降低(P0.01)；子宮系數(shù)顯著下降(P0.01),大鼠在敞箱中水平活動(dòng)次數(shù)顯著下降(P0.05)；體重緩慢顯著增加(P0.01),直腸溫度顯著下降(P0.05)。結(jié)論單去勢(shì)(8周)和去勢(shì)加腎虛大鼠,均模擬了更年期激素水平紊亂,子宮萎縮以及體溫和行為方面的變化。與單去勢(shì)(8周)比較,去勢(shì)加腎虛大鼠表現(xiàn)出的類(lèi)更年期下丘腦-垂體-性腺軸激素水平紊亂及植物神經(jīng)功能紊亂狀態(tài)更加典型。 2.2精烏膠囊對(duì)去勢(shì)加腎虛大鼠類(lèi)更年期綜合征狀態(tài)的干預(yù)作用 目的觀察精烏膠囊對(duì)去勢(shì)加腎虛大鼠類(lèi)更年期綜合征狀態(tài)的作用,并初步分析其作用機(jī)制。方法健康雌性,育齡未孕SD大鼠100只,隨機(jī)留取15只作為正常對(duì)照組,按2.1中方法建立去勢(shì)加腎虛大鼠模型,并隨機(jī)分為：模型組、精烏膠囊1.62、0.81、0.405g/kg劑量組,坤寶丸組(0.823g/kg)。按照相應(yīng)劑量每天灌胃給藥,連續(xù)8周后,觀察各組大鼠血清激素(E2、P、PRL、LH、FSH、GnRH)、臟器(子宮、腎、腎上腺、垂體、下丘腦、海馬)系數(shù)、行為(敞箱活動(dòng)、自主活動(dòng)、高架十字迷宮、戊巴比妥鈉誘導(dǎo)睡眠)、直腸溫度、血清氧化產(chǎn)物丙二醛(MDA)水平及腦內(nèi)(下丘腦、皮層)單胺類(lèi)神經(jīng)遞質(zhì)(去甲腎上腺素(NE)、多巴胺(DA)、5-羥吲哚乙酸(5-HIAA)、5-羥色胺(5-HT))水平的變化。結(jié)果模型大鼠各指標(biāo)變化如下：血清P、PRL水平顯著下降(P0.01)；子宮、垂體系數(shù)顯著下降(P0.01)；在敞箱中垂直活動(dòng)次數(shù)顯著下降(P0.05),自主活動(dòng)顯著下降(P0.05),高架十字迷宮中：總路程顯著下降(P0.01)；大鼠直腸溫度顯著降低(P0.01)；大鼠下丘腦NE水平顯著升高(P0.01),皮層5-HIAA水平顯著降低(P0.05)。與模型組比較,精烏膠囊各劑量組各指標(biāo)變化如下：大劑量顯著降低血清GnRH水平(P0.05),小劑量顯著降低血清P水平(P0.05)；大劑量子宮系數(shù)顯著下降(P0.05),大、中、小劑量腎系數(shù)顯著下降(P0.01或P0.05),中劑量腎上腺系數(shù)顯著下降(P0.05),大、中劑量下丘腦系數(shù)顯著下降(P0.05)；大劑量顯著升高水平活動(dòng)次數(shù)(P0.05),中劑量顯著降低水平活動(dòng)次數(shù)(P0.05),中、小劑量顯著升高垂直活動(dòng)次數(shù)(P0.01),大劑量顯著增加自主活動(dòng)次數(shù)(P0.01)；高架十字迷宮中：大劑量顯著增加進(jìn)開(kāi)臂區(qū)停留時(shí)間百分比(P0.05),中劑量顯著增加總路程、開(kāi)臂區(qū)停留時(shí)間百分比(P0.01或P0.05),小劑量顯著增加總路程(P0.01)；各劑量均可顯著降低大鼠血清MDA水平(P0.01)；小劑量下丘腦NE顯著升高(P0.01),大、小劑量顯著降低皮層DA水平(P0.01或P0.05),大、中、小劑量顯著降低皮層5-HIAA水平(P0.01),中劑量顯著降低5-HT水平(P0.01),大劑量顯著降低皮層5-HT(P0.05)結(jié)論精烏膠囊對(duì)去勢(shì)加腎虛動(dòng)物模型的改善作用不是通過(guò)促進(jìn)下丘腦-垂體-性腺軸的分泌,而是側(cè)重于調(diào)節(jié)中樞單胺類(lèi)神經(jīng)遞質(zhì)的失衡,從而改善行為等的異常。 3小結(jié) 1疾病動(dòng)物模型(去勢(shì))與病-證結(jié)合動(dòng)物模型(去勢(shì)加腎虛)相比較,二者均可模擬更年期激素水平紊亂,子宮萎縮、體重以及體溫的變化。但前者需掌握成模的時(shí)間,不宜過(guò)長(zhǎng),后者表現(xiàn)出類(lèi)更年期下丘腦-垂體-性腺軸激素水平紊亂狀態(tài)指標(biāo)、體溫改變,植物神經(jīng)功能紊亂以及腎虛表征等更典型,對(duì)評(píng)價(jià)中藥的作用更適用。 2精烏膠囊具有改善去勢(shì)加腎虛大鼠類(lèi)更年期綜合征狀態(tài)的作用。主要特點(diǎn)表現(xiàn)在以下幾個(gè)方面：對(duì)下丘腦-垂體-性腺軸血清相關(guān)激素?zé)o明顯促進(jìn)分泌作用；增加大鼠自主活動(dòng)次數(shù),消除焦慮情緒；調(diào)節(jié)中樞單胺類(lèi)神經(jīng)遞質(zhì)水平的紊亂。本研究結(jié)果為精烏膠囊的臨床應(yīng)用提供了部分實(shí)驗(yàn)依據(jù),其作用特點(diǎn)及深入的作用機(jī)制有待進(jìn)一步的研究。
[Abstract]:Climacteric syndrome (CS) is a series of syndrome (including tidal red tide fever, irritability, insomnia and forgetfulness, dizziness, headache, pain, low back pain, osteoporosis, etc.). The lowering of the estrogen level caused by the retreat, as well as the accompanying neuroendocrine disorder and the disturbance of the plant nerve function directly lead to the occurrence of menopause syndrome. The Jingwu capsule is mainly composed of 4 Chinese herbs, Radix Polygoni Multiflori, Huang Jing (system), Ligustrum lucidum (system), and dry lotus. It has the function of supplementing the liver and kidney, benefiting the blood and the strong muscles and bones. It is intended to be used in the climacteric synthesis. The treatment of signs.
This study explored the animal model of climacteric syndrome, established a disease syndrome combined animal model (castration plus kidney deficiency) which was closer to the clinical symptoms of climacteric syndrome, and simulated the clinical manifestations of menopause syndrome: changes in the hypothalamus pituitary - gonadal axis related hormones in the blood and the disorder of plant nerve function. The effects of Jing Wu Capsule on climacteric syndrome were observed, providing experimental basis for the clinical application of the compound.
1 to observe the changes of indexes in castrated rat models and the intervention effect of Jing Wu capsule.
Objective To observe the changes of the indexes of the castrated (10 week) model rats and the effect of Jing Wu Capsule on it. Methods healthy female and 90 SD rats of childbearing age were randomly left to take 15 normal controls. The other rats were removed from the bilateral ovary by operation. From day 6-10, the rats were smeared with vaginal smears every day, with no estrus reaction as castration. After 6 weeks of castration, rats were randomly divided into two groups: castration model group, three dose group of Jing Wu capsule 1.62,0.81,0.405g/kg, Kun Bao pill group (0.823g/kg), gavage according to the corresponding dosage, normal control group and castrated model group were given equal amount of drinking water for 4 weeks. Action, autonomic activity, the influence of pentobarbital sodium induced sleep time induced by sleep time and rectal temperature determination; after anaesthesia, blood was taken and whole blood viscosity was measured; some blood centrifuged serum, serum progesterone (P), estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), gonadotropin releasing hormone (GnRH) content, and the content of gonadotropin releasing hormone (GnRH). The changes of serum related hormones in the model group of the castrated (10 weeks) model group were: GnRH significantly decreased (P0.01), P content decreased 1/2 (the standard difference was large, no significant difference); the uterine coefficient decreased significantly (P0.01), the renal coefficient decreased significantly (P0.05); rectal temperature was significantly lower. The whole blood viscosity increased significantly (1s-1,30s-1,70s-1100s-1150s-1200s-1) (P0.01), and the whole blood viscosity increased significantly (P0.01). There was no obvious change in other indexes. Compared with the castrated (10 week) model group, the capsule of Jing Wu had a reversal effect on some indexes, showing the recovery of rectal temperature in each dose (P0.05 or P0.01), and the increase of GnRH content. The changes of serum E2, FSH, LH and PRL levels in the model rats were different from those in the literature. The preliminary analysis was related to the time point of the test, and the literature was more than 5-6 weeks after the castration. Conclusion because the castration model of this experiment did not show the typical clinical manifestation of the climacteric syndrome, it can not be used to evaluate the effect of Jing Wu capsule, only as a recipe. The exploration of law.
2 Establishment of a climacteric syndrome model with castration plus kidney deficiency and intervention of Jing Wu capsule
2.1 establishment of climacteric syndrome model with castration plus kidney deficiency rats
Objective to establish the model of castration and kidney deficiency rats to evaluate the characteristics of the model. Methods 50 healthy female SD rats and 15 rats of childbearing age were randomly selected as the normal control group. The other rats were removed by operation. After the castration, the rats were smeared with vaginal smears on day 5-7, and no estrus reaction was observed in rats. The rats were divided into single castration group, castration plus kidney deficiency group. The rats were injected with hydrocortisone (25mg/kg) daily for two weeks. The rats' body weight was measured every week. The rat tail root, underarm and foot temperature were measured at the completion of modeling. When Yu Jianmo finished, the model was modeled for 4 weeks, and the model of rat serum related hormone (E2, FSH, LH, GnRH) was established for 6 weeks; and the construction of the rat model was built. 1 weeks before the completion of the model, modeling was completed, modeling for 1 weeks, modeling for 2 weeks, modeling for 3 weeks, modeling for 5 weeks, modeling for 6 weeks and measuring rectal temperature in rats. After modeling for 6 weeks, the rats' behavior (open box activity, autonomic activity, pentobarbital induced sleep) and organs (uterus, kidney, adrenal) coefficient were observed in turn. The results were compared with the normal control group, and the single castrated rats were compared with the normal control group. The changes of the index were as follows: when the modeling was completed, the E2 was significantly decreased (P0.01) and the FSH was significantly increased (P0.05); the modeling was 4 weeks, and GnRH decreased significantly (P0.01); the modeling for 6 weeks, E2 significantly decreased (P0.05), LH significantly increased (P0.05), the uterus coefficient, the kidney coefficient significantly decreased (P0.01), the number of vertical movements in the open box increased significantly (P0.05); the weight of the rectum was rapidly increased and rectum increased. There was a slight decrease in temperature. The changes in the parameters of the ovariectomized and Kidney Asthenia Rats were as follows: when the modeling was completed, E2 and GnRH were significantly decreased (P0.01 or P0.05), FSH was significantly increased (P0.01), the underarm temperature of the rats increased significantly (P0.05 or P0.01), and the modeling for 4 weeks, E2 and GnRH decreased significantly (P0.05 or P0.01); modeling for 6 weeks .01); the uterine coefficient decreased significantly (P0.01), the level of horizontal activity in the open box decreased significantly (P0.05), the body weight slowed significantly (P0.01), and the rectal temperature decreased significantly (P0.05). Conclusion the single castration (8 weeks) and the ovariectomized and Kidney Asthenia Rats all simulated the changes in the hormone levels in the more years, the atrophy of the uterus, and the changes in body temperature and behavior. Compared with the single castration (8 weeks), the hypothalamus - hypophysis - gonadotropin level disorder and the state of the autonomic nervous dysfunction were more typical in the ovariectomized and kidney deficiency rats.
2.2 intervention effects of Jing Wu Capsule on climacteric syndrome in ovariectomized rats and kidney deficiency rats
Objective To observe the effect of Jing Wu Capsule on the state of menopause syndrome in castration and kidney deficiency rats and preliminary analysis of its mechanism. Methods 100 healthy female SD rats and 15 rats of childbearing age without pregnancy were randomly selected as normal control group, and the castrated and kidney deficiency rats were established by 2.1 methods and randomly divided into model group and Jing Wu capsule 1.62,0.81 0.405g/kg dose group and Kun Bao pill group (0.823g/kg). The serum hormone (E2, P, PRL, LH, FSH, GnRH) and the coefficient of the organs (uterus, kidney, adrenal, hypophysis, hypothalamus, hippocampus) of rats were observed for 8 weeks after the corresponding dose of the stomach, and the rectal temperature was (open box activity, elevated cross maze, pentobarbital induced sleep), and rectal temperature. The level of serum oxidation product malondialdehyde (MDA) and the level of monoamine neurotransmitter (norepinephrine (NE), dopamine (DA), 5- hydroxyindolo acetic acid (5-HIAA), 5- hydroxytryptamine (5-HT)) in the brain (hypothalamus, cortex). The results of the model rats were as follows: the serum P, the PRL level decreased significantly (P0.01), the uterus, the pituitary coefficient was significantly lower. Drop (P0.01); the number of vertical activities in the open box decreased significantly (P0.05), autonomous activity decreased significantly (P0.05), in the elevated cross maze, the total path decreased significantly (P0.01), the rectal temperature in rats decreased significantly (P0.01), the level of NE in the hypothalamus of rats was significantly increased (P0.01), and the level of cortical 5-HIAA decreased significantly (P0.05). The changes of the indexes in each dose group were as follows: the large dose significantly reduced the serum GnRH level (P0.05), the small dose significantly decreased the serum P level (P0.05), the large dose of the uterus coefficient decreased significantly (P0.05), the large, medium and small dose renal coefficient decreased significantly (P0.01 or P0.05), the medium dose adrenal coefficient decreased significantly (P0.05), and the medium dose hypothalamic coefficient was significant. Decrease (P0.05); large dose significantly increased level of activity (P0.05), middle dose significantly reduced the frequency of horizontal activity (P0.05), small dose significantly increased the number of vertical activity (P0.01), large dose significantly increased the number of autonomous activities (P0.01), high dose of the cross maze: large dose significantly increased the percentage of stay in the open arm area (P0.05). The median dose increased significantly, the percentage of staying time in the open arm area (P0.01 or P0.05), the small dose significantly increased the total road distance (P0.01), and the serum MDA level (P0.01) was significantly reduced in each dose, and the low dose of NE in the hypothalamus increased significantly (P0.01), and the small dose significantly decreased the level of DA (P0.01 or P0.05) in the cortex (P0.01 or P0.05), and the small dose decreased significantly. Cortical 5-HIAA level (P0.01), middle dose significantly reduced 5-HT level (P0.01), large dose significantly decreased cortical 5-HT (P0.05). Conclusion the effect of Jing Wu Capsule on castration and kidney deficiency animal model is not by promoting the secretion of hypothalamus pituitary gonadal axis, but focusing on regulating the imbalance of the central monoamine neurotransmitters, thus improving the behavior and so on. Abnormality.
3 Summary
1 the animal model of disease (castration) compared with the animal model of disease and syndrome (castration plus kidney deficiency), the two can simulate changes in hormone level disorder, uterine atrophy, body weight and body temperature in menopause. However, the former needs to master the time of mold formation and should not be too long, the latter shows the index of the level disorder of hypothalamus pituitary gonadal hormone levels in the more years. Changes in body temperature, autonomic nervous function and kidney deficiency are more typical, and are more suitable for evaluating the role of traditional Chinese medicine.
The 2 Jing Wu capsule has the effect of improving the state of menopause syndrome of rats with castration and kidney deficiency. The main characteristics are as follows: there is no obvious promotion of the secretion of serum related hormones in the hypothalamus pituitary gonadal axis, increasing the frequency of autonomic activity and eliminating anxiety, and regulating the turbulence of the level of monoamine neurotransmitters in the central nervous system. The results of this study provide some experimental evidence for clinical application of Jing Wu capsule, and its action characteristics and in-depth mechanism need to be further studied.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R271.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 梁文珍,劉道芳;紫參顆粒治療圍絕經(jīng)期綜合征臨床研究[J];安徽中醫(yī)學(xué)院學(xué)報(bào);2004年02期

2 趙勝;孔德明;;女貞子、淫羊藿、女貞子合淫羊藿對(duì)更年期模型大鼠作用的實(shí)驗(yàn)研究[J];貴陽(yáng)中醫(yī)學(xué)院學(xué)報(bào);2007年02期

3 董莉,呂嶸,洪巖,孟煒;補(bǔ)腎解郁清心方對(duì)更年期抑郁癥模型大鼠HPO軸及單胺類(lèi)神經(jīng)遞質(zhì)的影響[J];上海中醫(yī)藥大學(xué)學(xué)報(bào);2003年02期

4 劉福春;張家慶;李菊仙;丁光霞;朱德增;;羥基脲制造“陽(yáng)虛”動(dòng)物模型的研究[J];吉林中醫(yī)藥;1984年05期

5 黃淑瓊,林麗明,陳效暢;滋腎寧心湯治療絕經(jīng)前后諸癥療效觀察[J];吉林中醫(yī)藥;2003年08期

6 馮利,葛嘉峰,孟曉潤(rùn),王秀霞,褚維亞;坤寧安對(duì)去勢(shì)雌性大鼠血清生殖內(nèi)分泌值含量影響的實(shí)驗(yàn)研究[J];中醫(yī)藥信息;2000年04期

7 馬春瑜;;中醫(yī)治療更年期綜合征經(jīng)驗(yàn)及研究概況[J];中醫(yī)研究;2012年09期

8 汪文來(lái);趙紅霞;金香蘭;張玲;王震;盧賀起;岳廣欣;于崢;張立石;;大補(bǔ)陰丸及加減方對(duì)去卵巢更年期模型大鼠血清FSH、LH及體質(zhì)量、腎上腺指數(shù)的影響[J];中國(guó)中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志;2013年03期

9 李文艷;吳昆侖;姚佳晨;都樂(lè)亦;劉艷輝;;滋腎養(yǎng)陰湯對(duì)去卵巢大鼠性激素水平及抗氧化能力的影響[J];中成藥;2013年04期

，

本文編號(hào)：1958273