發(fā)布時間：2018-05-24 19:01

  本文選題：針灸 + 慢性鼻竇炎��； 參考：《成都中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：觀察針刺結(jié)合關(guān)元灸對小鼠慢性鼻竇炎(CRS)模型鼻竇黏膜中NF-kBp50蛋白及IFN-γ表達(dá)水平的影響,探索針刺結(jié)合關(guān)元灸治療CRS的可能機(jī)制以及慢性鼻竇炎發(fā)生發(fā)展的分子生物學(xué)機(jī)制。 方法：選用C57BL/6J小鼠160只,適應(yīng)性喂養(yǎng)一周后,按入室順序先后編號,采用DPS軟件將160小鼠隨機(jī)分為：正常組,模型組,假手術(shù)組,針灸組,西藥組,中藥常規(guī)劑量組,中藥低劑量組,中藥高劑量組,共8組,每組20只(本論文只納入針灸組、西藥組、假手術(shù)組、正常組、模型組5組數(shù)據(jù))。隨后進(jìn)行小鼠慢性鼻竇炎模型建立。造模后第1天進(jìn)行觀察,造模后第8周開始,針灸組給予針刺足三里與灸關(guān)元交替治療,西藥組給予克拉霉素治療。治療14天后,將小鼠麻醉后處死,在超凈冰面臺上取出鼻黏膜組織。在光鏡下觀察各組鼻黏膜的病理形態(tài)變化。以免疫組化SABC法測定NF-kBp50蛋白表達(dá)及丫-干擾素(IFN-γ)表達(dá)水平。 結(jié)果：1、癥狀及體征：模型組的鼻塞、流涕、噴嚏、搔抓鼻部癥狀仍然存在,并伴有溢淚及結(jié)膜充血水腫癥狀。針灸組、西藥組噴嚏、流涕癥狀有所改善,噴嚏及抓鼻次數(shù)較少,無溢淚及結(jié)膜充血水腫表現(xiàn)。2、病理HE切片示：模型組鼻黏膜呈慢性炎癥表現(xiàn),黏膜炎細(xì)胞浸潤,上皮細(xì)胞明顯增生,纖毛及上皮細(xì)胞排列紊亂,部分區(qū)域變性、壞死、脫落,形成多個小潰瘍。針灸組鼻黏膜上皮修復(fù)良好,炎細(xì)胞浸潤、腺體及杯狀細(xì)胞增生情況不明顯。3、模型組NF-κ Bp50蛋白在鼻黏膜中表達(dá)的平均光密度值增大,相比正常組及假手術(shù)組有顯著性差異(P0.01)；治療后,針灸組、西藥組NF-κ Bp50蛋白在鼻黏膜中表達(dá)的平均光密度值減小,較模型組有顯著性差異(P0.01)；2、模型組IFN-γ在鼻黏膜中表達(dá)水平相比正常組及假手術(shù)組無差異(P0.05)。治療后,針灸組、西藥組IFN-γ在鼻黏膜中表達(dá)的平均光密度值增大,較模型組有差異(P0.05)。 結(jié)論：針刺結(jié)合關(guān)元灸能夠有效改善慢性鼻竇炎模型小鼠的全身癥狀,降低慢性鼻竇炎小鼠鼻黏膜中NF-κBp50蛋白的表達(dá)水平,促進(jìn)IFN-γ的表達(dá),從而抑制多種炎性因子的產(chǎn)生,糾正Th1/Th2的失衡狀態(tài),提高慢性鼻竇炎模型小鼠的機(jī)體免疫能力,減輕鼻黏膜的炎癥反應(yīng),對炎性反應(yīng)導(dǎo)致的鼻-鼻黏膜損傷有一定修復(fù)作用；
[Abstract]:Objective: to observe the effect of acupuncture combined with Guanyuan moxibustion on the expression of NF-kBp50 protein and IFN- 緯 in sinusoidal mucosa of mice with chronic sinusitis. To explore the possible mechanism of acupuncture combined with Guanyuan moxibustion in the treatment of CRS and the molecular biological mechanism of the occurrence and development of chronic sinusitis. Methods: 160 C57BL/6J mice were randomly divided into normal group, model group, sham operation group, acupuncture group, western medicine group and traditional Chinese medicine group. There were 20 rats in each group in the low dose group and high dose group of traditional Chinese medicine (this paper only included acupuncture group, western medicine group, sham-operation group, normal group and model group). Then the model of chronic sinusitis in mice was established. The model was observed on the first day after the model was made. The acupuncture group was treated with acupuncture Zusanli alternately with moxibustion Guanyuan and the western medicine group was treated with clarithromycin from the 8th week after the model making. After 14 days of treatment, the mice were anesthetized and killed, and nasal mucosa was removed from the super-clean ice table. The pathological changes of nasal mucosa in each group were observed under light microscope. The expression of NF-kBp50 and IFN- 緯 were detected by immunohistochemical SABC method. Results 1, symptoms and signs: nasal congestion, runny, sneezing, scratching and scratching nose were still present in the model group, accompanied by tears and conjunctival hyperemia and edema. In acupuncture group and western medicine group, the symptoms of sneezing and runny were improved, the number of sneezing and scratching nose was less, there were no tears and conjunctival hyperemia edema. The pathological HE section showed that the nasal mucosa of model group showed chronic inflammation and mucositis cell infiltration. Epithelial cells proliferated, cilia and epithelial cells were arranged in disorder, some areas were denatured, necrotized, shed, and many small ulcers were formed. In the acupuncture and moxibustion group, the nasal mucosal epithelium was repaired well, inflammatory cells infiltrated, glandular and goblet cell hyperplasia was not obvious. The average optical density of NF- 魏 Bp50 protein expression in the nasal mucosa in the model group was increased. After treatment, the average optical density of NF- 魏 Bp50 protein expression in nasal mucosa of acupuncture group and western medicine group decreased. Compared with the model group, there was a significant difference between the model group and the model group (P 0.01). The expression level of IFN- 緯 in the nasal mucosa of the model group was not different from that of the normal group and the sham operation group (P 0.05). After treatment, the mean optical density of IFN- 緯 expression in nasal mucosa of acupuncture group and western medicine group was increased, which was different from that of model group (P 0.05). Conclusion: acupuncture combined with Guanyuan moxibustion can effectively improve the systemic symptoms of mice with chronic sinusitis, decrease the expression of NF- 魏 Bp50 protein in nasal mucosa of chronic sinusitis mice, promote the expression of IFN- 緯, and thus inhibit the production of various inflammatory factors. To correct the imbalance of Th1/Th2, to improve the immune ability of mice with chronic sinusitis, to alleviate the inflammatory reaction of nasal mucosa, and to repair the rhino-nasal mucosal injury caused by inflammatory reaction.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R246.81

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