本文選題：蝦青素 + 精子�。� 參考：《南方醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:觀察蝦青素(AST)對(duì)奧硝唑(ORN)致雄性大鼠生殖功能損傷的保護(hù)作用,并探討其初步作用機(jī)制,為其臨床治療不育提供理論依據(jù)。方法:取40只成年雄性SD大鼠,隨機(jī)分成5組,每組8只,分別為:A組,0.5%羧甲基纖維素鈉溶液+1 ml玉米油;B組,ORN 400 mg/(kg · d);C組,ORN 800 mg/(kg · d);D 組,ORN 400 mg/(kg · d)+ AST 20 mg/(kg · d);E 組,ORN 800 mg/(kg · d)+ AST 20 mg/(kg · d),灌胃。實(shí)驗(yàn)期間記錄大鼠一般情況,3周后水合氯醛腹腔麻醉,剝離大鼠睪丸、附睪、精囊腺組織并稱重,取一側(cè)附睪尾部檢測(cè)精子濃度和活力,剩余部分和一側(cè)睪丸組織做病理切片觀察;另一側(cè)附睪組織勻漿,檢測(cè)其GSH-Px、GR、CAT、SOD活性及MDA含量;另一側(cè)睪丸組織勻漿后做組織8-OHdG含量和LDH、SDH、γ-GT活性檢測(cè)。結(jié)果:1.實(shí)驗(yàn)期間A組大鼠活動(dòng)反應(yīng)正常,體重增加明顯,皮毛有光澤。B、C組兩組大鼠逐漸出現(xiàn)精神萎靡、反應(yīng)變慢、嗜睡等多種癥狀,且隨給藥時(shí)間增加而加重。D、E組大鼠給予AST治療后癥狀均得到改善。2.大鼠睪丸附睪HE結(jié)果顯示,A組睪丸生精小管管腔內(nèi)各級(jí)生精細(xì)胞排列緊密整齊,結(jié)構(gòu)清晰,核染色致密均勻;附睪管管腔內(nèi)見(jiàn)大量正常精子,纖毛排列整齊緊密。B、D組大鼠的睪丸和附睪組織形態(tài)與A組相比基本無(wú)差異。C組大鼠睪丸生精小管內(nèi)的生精細(xì)胞明顯減少,層次不明,細(xì)胞間隙變大,空泡增多;附睪管管腔內(nèi)精子數(shù)量明顯減少、排列疏松紊亂,可見(jiàn)多種非精子細(xì)胞成分和大片空白無(wú)染區(qū)域。E組大鼠睪丸組織結(jié)構(gòu)有所恢復(fù),附睪管內(nèi)精子數(shù)量有所增加,但仍與A組有一定差距。3.與A組相比,B組的附睪尾精子活力,附睪GSH-Px、SOD活性顯著降低,MDA含量顯著上升(P0.05),其他指標(biāo)改變不明顯;C組的附睪尾精子活力、精子濃度、睪丸系數(shù),附睪組織GSH-Px、GR、CAT、SOD活性和睪丸組織LDH、SDH、γ-GT活性均顯著降低,而附睪MDA和睪丸8-OHdG含量顯著上升(P0.05)。AST治療后:與B組相比,D組附睪尾精子活力和附睪組織SOD活性顯著增加,而MDA含量顯著下降(P0.05);與C組相比,E組實(shí)驗(yàn)前后體重增量、精子活力、睪丸LDH、SDH活性顯著增加,而MDA含量顯著下降(P0.05),其他指標(biāo)有所好轉(zhuǎn)但無(wú)統(tǒng)計(jì)學(xué)差異。結(jié)論:AST可提高ORN所致的生殖損傷大鼠的精子質(zhì)量,尤其是精子活力,其機(jī)制可能與提高大鼠附睪組織的抗氧化能力和睪丸標(biāo)志酶的活性及抑制細(xì)胞NDA損傷有關(guān)。
[Abstract]:Objective: to observe the protective effect of astaxanthin (AST) on reproductive function injury induced by ornidazole (ORN) in male rats, and to explore its primary mechanism, and to provide theoretical basis for clinical treatment of sterility. Methods: forty adult male SD rats were randomly divided into 5 groups with 8 rats in each group. The rats in group B were treated with 0.5% sodium carboxymethylcellulose solution of 0.5% sodium carboxymethyl cellulose. Group B was given oral administration of ORN400 mg/(kg DX, group C, ORN800 mg/(kg DX, group D, ORN400 mg/(kg, AST 20 mg/(kg, group E, AST 20 mg/(kg d, AST 20 mg/(kg dl, respectively. During the experiment, the rats were anesthetized with chloral hydrate for 3 weeks, the testis, epididymis and seminal vesicle were removed and weighed. The sperm concentration and motility were measured by the tail of one epididymis. The rest of testis and one side of testis were observed by pathological section, the other side of epididymis homogenate was examined for the activity of SOD and MDA in GRCATU, and the content of 8-OHdG and activity of 8-OHdG and 緯 -GT in homogenate of the other side of testis were detected. The result is 1: 1. During the experiment, the rats in group A had normal activity reaction, weight gain was obvious, and the rats of group C with lustrous fur gradually developed many symptoms, such as mental retardation, slow reaction, drowsiness and so on. With the increase of administration time, the symptoms of rats in group .DnE were improved after AST treatment. The results of HE showed that the spermatogenic cells in the seminiferous tubule of the testis in group A were arranged neatly, the structure was clear, the nucleus staining was dense and uniform, and a large number of normal spermatozoa were found in the cavities of the epididymis. The morphology of testis and epididymis in group D was similar to that in group A. the spermatogenic cells in testicular seminiferous tubules of group C were obviously decreased, the level of spermatogenic tubules was not clear, the intercellular space became larger and the vacuoles increased. The number of spermatozoa in the epididymal duct was decreased obviously and the arrangement was loose. It was found that the structure of testis in group E was recovered and the number of spermatozoa in epididymal duct was increased. However, there was still a certain gap between group A and group A. Compared with group A, the sperm motility of cauda epididymidis in group B and the activity of GSH-PxX SOD in epididymal cauda significantly decreased the content of MDA increased significantly (P 0.05). The other indexes did not change significantly in group C, such as sperm motility, sperm concentration and testis coefficient. The activity of SOD in epididymal tissue and the activity of LDH- SDH, 緯 -GT in testicular tissue decreased significantly, while the contents of MDA and 8-OHdG in testis increased significantly after treatment with P0.05. AST: compared with group B, the sperm motility of caudal epididymis and the activity of SOD in epididymal tissue in group D were significantly increased. Compared with group C, the weight increment, sperm motility and the activity of MDA in testis increased significantly in group E, while the content of MDA decreased significantly (P 0.05), but there was no statistical difference between group E and group C. Conclusion the effects of ORN on sperm quality, especially sperm motility, may be related to the increase of antioxidant capacity and testicular marker enzyme activity of rat epididymis and the inhibition of NDA damage.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R698.2

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