本文選題：地塞米松 + 生長期�。� 參考：《中南大學》2014年碩士論文

【摘要】：目的：觀察小劑量地塞米松對生長期小鼠骨代謝的影響。 方法：24只4周齡的雌性C57B1/6J小鼠隨機分兩組,每組各12只：地塞米松組(DEX組),右側(cè)股四頭肌肌注1mg/kg體重地塞米松；對照組。每周稱量小鼠體重并記錄。所有小鼠干預4周后予以腹腔注射巴比妥麻醉,頸椎脫臼法處死小鼠后立即摘眼球取血,分離血清,酶聯(lián)免疫吸附法(enzyme linked immunosorbent assay, ELISA)檢測Ⅰ型前膠原N端前肽(PINP)和骨Ⅰ型膠原交聯(lián)C端頂端肽(CTX-Ⅰ)蛋白的表達水平；取一側(cè)脛骨行顯微CT掃描分析,掃描完成后進行三維重建,用顯微CT自帶的MicroView2.0+ABA軟件對松質(zhì)骨表觀骨密度、組織骨密度、骨體積分數(shù)、骨面積分數(shù)、骨小梁厚度、骨小梁間隔、骨小梁數(shù)量、結(jié)構(gòu)模型指數(shù)等指標進行分析。并建立各組松質(zhì)骨二維圖像；免疫組化(immunohistochemistry IHC)方法檢測脛骨干骺端骨保護素(osteoprotegerin OPG)、核因子-κB受體活化因子配體(receptor activator for nuclear factor-κB ligand, RANKL)蛋白表達；抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase TRAP)染色方法檢測破骨細胞。 結(jié)果：與對照組相比,DEX組小鼠脛骨干骺端表觀骨密度及骨體積分數(shù)明顯增高(p0.05),而血清PINP及β-CTX水平明顯下降(p0.05)。地塞米松促進小鼠脛骨干骺端OPG蛋白表達,而對RANKL表達無明顯影響,導致OPG/RANKL比例顯著升高；與對照組相比,DEX組小鼠脛骨干骺端破骨細胞數(shù)量明顯減少。 結(jié)論：本研究發(fā)現(xiàn),小劑量的地塞米松可能對生長期小鼠骨量增長有促進作用,其機制可能在于通過調(diào)節(jié)OPG/RANKL的比例,抑制骨吸收,降低骨轉(zhuǎn)換率。
[Abstract]:Objective: to observe the effect of low dose dexamethasone on bone metabolism in growing mice. Methods Twenty four four-week-old female C57B1/6J mice were randomly divided into two groups: dexamethasone group (n = 12), dexamethasone (1mg/kg) in right quadriceps femoris muscle group, and control group (n = 12). The mice were weighed weekly and recorded. After 4 weeks of intervention, all the mice were given intraperitoneal injection of barbitole anesthesia, and the mice were killed by cervical dislocated method. The blood was taken from the eyeball and the serum was separated immediately after the mice were killed. Enzyme linked immunosorbent assay (linked immunosorbent assay, ELISA) was used to detect the expression of PINP- 鈪，

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