本文選題：地塞米松 + 生長(zhǎng)期�。� 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：觀察小劑量地塞米松對(duì)生長(zhǎng)期小鼠骨代謝的影響。 方法：24只4周齡的雌性C57B1/6J小鼠隨機(jī)分兩組,每組各12只：地塞米松組(DEX組),右側(cè)股四頭肌肌注1mg/kg體重地塞米松；對(duì)照組。每周稱量小鼠體重并記錄。所有小鼠干預(yù)4周后予以腹腔注射巴比妥麻醉,頸椎脫臼法處死小鼠后立即摘眼球取血,分離血清,酶聯(lián)免疫吸附法(enzyme linked immunosorbent assay, ELISA)檢測(cè)Ⅰ型前膠原N端前肽(PINP)和骨Ⅰ型膠原交聯(lián)C端頂端肽(CTX-Ⅰ)蛋白的表達(dá)水平；取一側(cè)脛骨行顯微CT掃描分析,掃描完成后進(jìn)行三維重建,用顯微CT自帶的MicroView2.0+ABA軟件對(duì)松質(zhì)骨表觀骨密度、組織骨密度、骨體積分?jǐn)?shù)、骨面積分?jǐn)?shù)、骨小梁厚度、骨小梁間隔、骨小梁數(shù)量、結(jié)構(gòu)模型指數(shù)等指標(biāo)進(jìn)行分析。并建立各組松質(zhì)骨二維圖像；免疫組化(immunohistochemistry IHC)方法檢測(cè)脛骨干骺端骨保護(hù)素(osteoprotegerin OPG)、核因子-κB受體活化因子配體(receptor activator for nuclear factor-κB ligand, RANKL)蛋白表達(dá)；抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase TRAP)染色方法檢測(cè)破骨細(xì)胞。 結(jié)果：與對(duì)照組相比,DEX組小鼠脛骨干骺端表觀骨密度及骨體積分?jǐn)?shù)明顯增高(p0.05),而血清PINP及β-CTX水平明顯下降(p0.05)。地塞米松促進(jìn)小鼠脛骨干骺端OPG蛋白表達(dá),而對(duì)RANKL表達(dá)無明顯影響,導(dǎo)致OPG/RANKL比例顯著升高；與對(duì)照組相比,DEX組小鼠脛骨干骺端破骨細(xì)胞數(shù)量明顯減少。 結(jié)論：本研究發(fā)現(xiàn),小劑量的地塞米松可能對(duì)生長(zhǎng)期小鼠骨量增長(zhǎng)有促進(jìn)作用,其機(jī)制可能在于通過調(diào)節(jié)OPG/RANKL的比例,抑制骨吸收,降低骨轉(zhuǎn)換率。
[Abstract]:Objective: to observe the effect of low dose dexamethasone on bone metabolism in growing mice. Methods Twenty four four-week-old female C57B1/6J mice were randomly divided into two groups: dexamethasone group (n = 12), dexamethasone (1mg/kg) in right quadriceps femoris muscle group, and control group (n = 12). The mice were weighed weekly and recorded. After 4 weeks of intervention, all the mice were given intraperitoneal injection of barbitole anesthesia, and the mice were killed by cervical dislocated method. The blood was taken from the eyeball and the serum was separated immediately after the mice were killed. Enzyme linked immunosorbent assay (linked immunosorbent assay, ELISA) was used to detect the expression of PINP- 鈪，

本文編號(hào)：1894574