本文選題：跖疣 + HPV��； 參考：《南京中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：跖疣是臨床上常見的,好發(fā)于足底的尋常疣。西醫(yī)對于多發(fā)性跖疣缺乏滿意療法。迅速有效地消除疣體,控制疼痛,減少復(fù)發(fā)率,是從事皮膚科工作的臨床醫(yī)生急切需要解決的重要課題。本課題以中醫(yī)基礎(chǔ)理論為指導(dǎo),結(jié)合導(dǎo)師臨床經(jīng)驗(yàn)總結(jié)解毒祛疣方,通過實(shí)驗(yàn)觀察解毒祛疣方對HPV感染表皮中朗格漢斯細(xì)胞的影響,為臨床治療提供一定的理論依據(jù)。 方法：8例HPV感染的尖銳濕疣(CA)患者均為我院皮膚科門診患者,均為初發(fā)患者,未經(jīng)過任何治療。對照組：8例對照為同時期我院男科包皮手術(shù)非CA患者。征得患者知情同意后,局部消毒麻醉,分別切取疣體／正常組織各8例,每例組織等分為10份。其中一份直接10%甲醛溶液固定,石蠟包埋,將9份真皮側(cè)向下,放入少量RPMI-1640培養(yǎng)液及濃度分別為0.025g/ml、0.05g/ml的藥液,只將疣體底部浸于培養(yǎng)液中。分別以37℃℃、42℃℃和45℃三個溫度照射30mmin后,PBS沖洗后,將疣體全部浸于RPMI-1640培養(yǎng)液,37℃C培養(yǎng)箱孵育12小時后將組織取出,10%甲醛溶液固定,石蠟包埋。常規(guī)石蠟切片,免疫組化染色后,觀察LC形態(tài)變化,數(shù)量變化。 結(jié)果：1.解毒祛疣方治療后HPV感染表皮中LC形態(tài)發(fā)生變化,細(xì)胞結(jié)構(gòu)不完整,樹突狀結(jié)構(gòu)減少、變短或消失。2.不同溫?zé)�、中藥濃度治療�?表皮內(nèi)LC的數(shù)量變化,隨溫度、濃度升高,LC數(shù)量逐漸減少,差異具有顯著性(P0.01)；HPV感染組織比正常組織表皮內(nèi)LC減少更明顯,也隨溫度、濃度變化而變化。正常人和HPV感染組織相同溫度、濃度之間的LC數(shù)量經(jīng)統(tǒng)計學(xué)處理,也具有顯著性差異(P0.01)。
[Abstract]:Objective: metatarsal warts are common in clinic. Western medicine lacks satisfactory treatment for multiple warts. It is urgent for clinicians engaged in dermatology to eliminate warts quickly and effectively, control pain and reduce recurrence rate. Under the guidance of the basic theory of traditional Chinese medicine, combined with the tutor's clinical experience, the author summarized the prescription of detoxification and dispelling warts, observed the effect of jiedu Quyoufang on Langerhans cells in HPV infected epidermis, and provided some theoretical basis for clinical treatment. Methods eight cases of HPV infected condyloma acuminatum were all outpatients in dermatology department of our hospital. The control group consisted of 8 cases of non-CA patients undergoing male prepuce surgery in the same period. After obtaining the informed consent of the patient, 8 cases of warts and 8 cases of normal tissue were cut out, and each case was divided into 10 parts. One of them was immobilized in 10% formaldehyde solution and embedded in paraffin wax. Nine parts of dermis were placed sideways down into a small amount of RPMI-1640 medium and 0.025 g / ml of 0.05 g / ml respectively. The verruca was only immersed in the medium at the bottom of the verruca. The warts were incubated in RPMI-1640 incubator at 37 鈩，

本文編號：1866019