發(fā)布時間：2018-04-30 05:13

  本文選題：左氧氟沙星注射液 + 纖毛　； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的 研究氣管內(nèi)注入左氧氟沙星注射液后大鼠氣管粘膜纖毛受損情況和肺組織病理改變及肺組織中IL-6蛋白的表達(dá)變化，明確氣管內(nèi)注入左氧氟沙星注射液對大鼠氣管粘膜超微結(jié)構(gòu)和肺組織的損傷及鹽酸氨溴索注射液對這種損傷的保護(hù)作用。方法 將健康雄性清潔級SD大鼠90只隨機(jī)分為三組，分別為正常對照組30只，，左氧氟沙星組30只，左氧氟沙星+氨溴索干預(yù)組30只。大鼠腹腔麻醉后，左氧氟沙星組、左氧氟沙星+氨溴索干預(yù)組各大鼠按2.8mg/100g經(jīng)環(huán)甲膜穿刺向氣管內(nèi)一次性緩慢注入左氧氟沙星注射液，氨溴索干預(yù)組在向氣管內(nèi)注入左氧氟沙星注射液前6h按70mg／kg向腹腔內(nèi)一次性注入鹽酸氨溴索注射液。氣管內(nèi)注藥后2小時、1天、2天、3天、5天、7天6個不同時間點分別放血處死5只大鼠，迅速剖取大鼠中下段氣管及右肺后葉組織。電鏡觀察中下段氣管纖毛的受損情況，光鏡觀察右肺后葉組織病理變化，免疫組化方法檢測肺組織IL-6蛋白的表達(dá)情況。 結(jié)果 1.氣管粘膜纖毛的損傷 1.1觀察正常對照組纖毛排列整齊、致密，無倒伏、水腫、壞死脫落，左氧氟沙星組和氨溴索干預(yù)組除第7天外，其余各時間點纖毛均出現(xiàn)不同程度倒伏、水腫、壞死脫落。 1.2左氧氟沙星組在第2小時、1天、2天、3天、5天與正常對照組比較，氣管粘膜纖毛均存在不同程度損傷，具有顯著性差異（P0.05）。 1.3左氧氟沙星組中，隨著時間的推移，氣管粘膜纖毛損傷程度逐漸加重，在氣管內(nèi)給藥后第3天時受損程度達(dá)最嚴(yán)重程度，各時間點間均具有統(tǒng)計學(xué)差異（P0.05）。 1.4氨溴索干預(yù)組在第2小時、2天、3天、5天時纖毛損傷程度與左氧氟沙星組比較，均有所減輕（P0.05）。 2.肺組織病理改變 2.1正常對照組支氣管上皮細(xì)胞排列整齊，支氣管腔內(nèi)無滲出物。左氧氟沙星組出現(xiàn)不同程度支氣管上皮細(xì)胞破壞，肺泡間隔內(nèi)可見明顯炎性細(xì)胞浸潤，肺泡腔內(nèi)出現(xiàn)滲出液、紅細(xì)胞、炎癥細(xì)胞，第7天基本恢復(fù)正常。 2.2左氧氟沙星組各時間點肺組織損傷程度比較中，第1天明顯重于第2小時（P0.05），第2天達(dá)到最嚴(yán)重程度，第3天起開始下降，第7天基本恢復(fù)正常。 2.3氨溴索干預(yù)組各時間點中，在第2小時、2天、3天、5天與左氧氟沙星比較，肺組織損傷均有明顯減輕（P0.05）。 3.IL-6蛋白的表達(dá)變化 3.1正常對照組肺組織IL-6蛋白少量表達(dá)，主要表達(dá)于支氣管上皮細(xì)胞細(xì)胞質(zhì)中，染色呈淡黃。左氧氟沙星組除第7天外，其余各時間點與正常對照組比較均具有顯著性差異（P0.05）。 3.2左氧氟沙星組從第1天起大部分支氣管上皮細(xì)胞細(xì)胞質(zhì)均染色，呈棕褐色，第5天起染色變淺，第7天著色呈淡黃色。 3.3氨溴索干預(yù)組第1天、7天IL-6蛋白表達(dá)量與左氧氟沙星組相比無統(tǒng)計學(xué)意義，其余各時間點比較均有明顯減少（P0.05）。 結(jié)論 1.氣管內(nèi)注入左氧氟沙星注射液對大鼠氣管粘膜和肺組織均能產(chǎn)生損傷作用，引起肺組織IL-6蛋白表達(dá)增加，IL-6蛋白表達(dá)增加可能參與了這種損傷過程。 2.鹽酸氨溴索注射液對保護(hù)氣管粘膜和肺組織的損傷有一定的作用，能降低肺組織IL-6蛋白的表達(dá)量。
[Abstract]:objective
The damage of trachea mucociliary, pathological changes of lung tissue and the expression of IL-6 protein in lung tissue were studied in rats after intratracheal injection of Levofloxacin Injection, and the damage of tracheal ultrastructure and lung tissue in the trachea and the protection of Ambroxol Hydrochloride Injection on the injury by intratracheal injection of Levofloxacin Injection were studied. Action. Method
90 healthy male clean SD rats were randomly divided into three groups, 30 in the normal control group, 30 in the levofloxacin group and 30 in the left ofloxacin + ambroxol intervention group. After the abdominal anesthesia, the left ofloxacin group and the left ofloxacin + ambroxol intervention group were injected into the trachea by 2.8mg/ 100g through the paracentesis to the trachea at one time. Levofloxacin Injection, ambroxol intervention group injected Levofloxacin Injection into the abdominal cavity before injection of Levofloxacin Injection into the trachea and injected Ambroxol Hydrochloride Injection into the abdominal cavity at one time. After 2 hours, 1 days, 2 days, 3 days, 5 days, 7 days and 6 different time points, 5 rats were injected into the trachea, and the middle and lower middle trachea and right lung of the rat were quickly dissected. The damage of the endotracheal cilium in the middle and lower middle segment was observed by electron microscopy. The pathological changes of the posterior lobe of the right lung were observed by light microscopy. The expression of IL-6 protein in the lung tissue was detected by immunohistochemical method.
Result
1. injury of trachea mucociliary
1.1 the cilia in the normal control group was arranged neatly, dense, no lodging, edema, necrosis and shedding, and the left ofloxacin group and ambroxol intervention group had different degrees of lodging, edema, necrosis and shedding in the other time points except seventh days.
1.2 levofloxacin group in second hours, 1 days, 2 days, 3 days, 5 days compared with the normal control group, the tracheal cilium had different degrees of damage, with significant difference (P0.05).
In 1.3 levofloxacin group, the damage degree of cilium in trachea mucous membrane was gradually increased with time, and the degree of damage reached the most serious degree at third days after administration in trachea, and there was statistical difference between each time point (P0.05).
1.4 in the ambroxol intervention group, the ciliary damage was reduced in second hours, 2 days, 3 days, 5 days compared with levofloxacin group (P0.05).
2. pathological changes of lung tissue
2.1 the bronchial epithelial cells in the normal control group were arranged neatly and there were no exudates in the bronchoalveolar cavity. There were different degrees of bronchial epithelial cell destruction in the levofloxacin group, obvious infiltration of inflammatory cells in the alveolar septum, exudation in the alveolar cavity, red blood cells and inflammatory cells, and the seventh days were basically restored to normal.
The lung tissue damage at each time point of 2.2 levofloxacin group was compared in the first days (P0.05), the second day was the most serious, the third days began to decline, and the seventh days were basically restored to normal.
2.3 in Ambroxol intervention group, lung injury was significantly reduced at second hours, 2 days, 3 days, 5 days compared with levofloxacin (P0.05).
Expression changes of 3.IL-6 protein
3.1 the expression of IL-6 protein in the lung tissue of the normal control group was mainly expressed in the cytoplasm of the bronchial epithelial cells, and the staining was yellowish. The left ofloxacin group was significantly different from that of the normal control group (P0.05) except for the normal control group (P0.05).
3.2 from the first day, the cytoplasm of most of the bronchial epithelial cells was stained brown, fifth days, and the coloring was pale yellow in seventh days.
3.3 on the first day of ambroxol intervention group, the expression of IL-6 protein was not significantly different from that of levofloxacin group on the 7 day, while the rest time points decreased significantly (P0.05).
conclusion
1. intratracheal injection of Levofloxacin Injection can damage the trachea mucous membrane and lung tissue of rats. The expression of IL-6 protein in lung tissue is increased. The increase of IL-6 protein may be involved in the damage process.
2. Ambroxol Hydrochloride Injection has a certain effect on the protection of tracheal mucosa and lung tissue damage, and can reduce the expression of IL-6 protein in lung tissue.

【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965

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