本文選題：內(nèi)毒素血癥 + 脂肪酸　； 參考：《營(yíng)養(yǎng)學(xué)報(bào)》2015年05期

【摘要】：目的明確內(nèi)毒素血癥中心臟功能改變和可能涉及的脂肪酸代謝途徑變化的機(jī)制,探討AMPK(單磷酸腺苷活化蛋白激酶AMP-activated protein kinase)及下游靶點(diǎn)在心肌組織能量貯存與消耗調(diào)節(jié)中的作用。方法 SD大鼠尾靜脈注射LPS(5mg/kg)建立內(nèi)毒素血癥模型,12h后麻醉,經(jīng)頸總動(dòng)脈置管監(jiān)測(cè)心功能:左心室內(nèi)壓(LVSP)、左心室舒張末壓(LVEDP)、左心室壓力上升/下降最大速率(±dp/dtmax),進(jìn)行心肌和血清游離脂肪酸檢測(cè),應(yīng)用western blot方法測(cè)定AMPKα,p-AMPKα,肌型-肉毒堿脂酰轉(zhuǎn)移酶1(muscle carnitine pamitoyltransferase1,m-CPT1),乙酰輔酶A羧化酶(acety1 coenzyme A carboxylase,ACCase),p-ACCase蛋白變化。結(jié)果內(nèi)毒素血癥組(LPS組)大鼠在注射LPS 12h后,+dp/dtmax,-dp/dtmax,和LVSP與正常對(duì)照組相比明顯降低,而LVEDP則明顯上升。內(nèi)毒素血癥組血清及心肌游離脂肪酸均高于正常組。兩組之間AMPKα和ACCase蛋白表達(dá)無顯著差異,而p-AMPKα和p-ACCase表達(dá)在內(nèi)毒素血癥大鼠組中明顯增高,同時(shí)m-CPT1蛋白表達(dá)明顯下降。結(jié)論內(nèi)毒素血癥心肌損傷與脂肪酸代謝紊亂有關(guān),涉及到m-CPT1,p-ACCase和p-AMPKα蛋白表達(dá)的變化。
[Abstract]:Objective to identify the changes in the changes in the central function of endotoxemia and the possible changes in the metabolic pathway of fatty acids, and to explore the role of AMPK (adenosine monophosphate activated protein kinase AMP-activated protein kinase) and the downstream target in the regulation of energy storage and consumption in myocardial tissue. LPS (5mg/kg) was injected into the tail vein of SD rats to establish endotoxin. The hypotheemia model, after 12h anesthesia, was used to monitor cardiac function through the common carotid artery catheterization: left ventricular pressure (LVSP), left ventricular end diastolic pressure (LVEDP), the maximum rate of left ventricular pressure rising / decreasing (+ dp/dtmax), myocardial and serum free fatty acid detection, AMPK a, p-AMPK a, and muscle type carnitine transferase 1 (muscle) by Western blot method. Carnitine pamitoyltransferase1, m-CPT1), acetyl coenzyme A carboxylase (acety1 coenzyme A carboxylase, ACCase), and p-ACCase protein changes. Results endotoxemia group (LPS group) rats were significantly lower than normal control group after injection. There was no significant difference in the expression of AMPK alpha and ACCase protein between the two groups, but the expression of p-AMPK alpha and p-ACCase was significantly increased in the rats with endotoxemia, and the expression of m-CPT1 protein was significantly decreased. Conclusion the myocardial injury of endotoxemia is related to the disorder of fatty acid metabolism, which involves m-CPT1, p-ACCase and p-AMPK alpha. Changes in protein expression.

【作者單位】： 浙江大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院重癥監(jiān)護(hù)室;浙江大學(xué)醫(yī)學(xué)院附屬邵逸夫醫(yī)院全科醫(yī)學(xué)科;
【基金】：國(guó)家自然基金(No.81101445) 浙江省衛(wèi)生廳醫(yī)藥衛(wèi)生項(xiàng)目(No.2013KYA063)
【分類號(hào)】：R541

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