本文選題：慢性前列腺炎 + 神經(jīng)內(nèi)分泌細胞��； 參考：《安徽醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的通過對神經(jīng)內(nèi)分泌細胞分泌物P物質(zhì)的研究來探討大鼠神經(jīng)內(nèi)分泌細胞與慢性前列腺炎之間的相關(guān)性。 方法選取40只2月齡的SPF級健康雄性Sprague-Dawley（SD）大鼠作為實驗對象,天平秤重，體重均在220-275g之間，，然后按體重排序編號，完全隨機分為兩組：模型組和對照組，每組20只，均在無菌環(huán)境中常規(guī)飼養(yǎng)。其中模型組SD大鼠經(jīng)10%的水合氯醛麻醉后，在無菌條件下經(jīng)腹行去勢手術(shù)，手術(shù)當(dāng)天及術(shù)后連續(xù)2天給予青霉素5萬U/(kg·d)肌肉注射,一周后觀察傷口無感染，愈合良好，開始背部皮下注射17β-雌二醇0.25mg/kg，連續(xù)注射30天；對照組SD大鼠未行去勢手術(shù)，而在同一時間于背部皮下連續(xù)注射0.9%生理鹽水一月。在最后一次注射17β-雌二醇和生理鹽水的24小時以內(nèi)，及時在無菌條件下運用心臟穿刺采血法收集5ml全血，然后迅速取出前列腺組織，放置于D-Hanks液中，再仔細剔除前列腺包膜及周圍脂肪、血管等組織，以獲得較為純凈的前列腺組織，最后用4%的多聚甲醛固定前列腺組織，并放入-80℃冰箱中備用。其中一部分前列腺組織經(jīng)過固定、石蠟包埋、脫水、透明、浸蠟和切片后，行HE染色，光鏡觀察，發(fā)現(xiàn)模型組前列腺間質(zhì)充血水腫，腺腔細胞不均勻增生，腺泡腔內(nèi)和周圍見大量炎癥細胞浸潤；而對照組SD大鼠的前列腺間質(zhì)無水腫,無炎癥細胞浸潤，腺泡上皮排列整齊，無增生現(xiàn)象。這表明SD大鼠慢性前列腺炎模型建造成功；而另一部分前列腺組織則經(jīng)過上述的一系列處理后用于免疫組織化學(xué)實驗；運用心臟穿刺法收集的全血經(jīng)過離心后，取其上清液移入無菌瓶內(nèi)，然后采用雙抗體夾心ELISA法測定兩組SD大鼠前列腺組織中P物質(zhì)表達的差異，并觀察此差異在統(tǒng)計學(xué)意義上是否具有顯著性。 結(jié)果免疫組化結(jié)果：模型組中P物質(zhì)染色陽性細胞數(shù)較對照組顯著增加且大都圍繞在前列腺管周圍，而對照組散在存在少量的P物質(zhì)染色陽性細胞，其中模型組及對照組中P物質(zhì)的陽性表達率分別為85%(其中弱陽性3例,陽性8例,強陽性6例)和20%(其中弱陽性2例,陽性2例)，進行組間比較，發(fā)現(xiàn)差異具有統(tǒng)計學(xué)意義(P0.01)；ELISA結(jié)果：模型組中P物質(zhì)的含量為(113.5±17.5)ng/ml；對照組中P物質(zhì)的含量為(98.0±18.1)ng/ml，組間比較，差異在統(tǒng)計學(xué)上具有顯著性（t=2.742,P=0.009）。結(jié)論本實驗結(jié)果顯示模型組SD大鼠血清及前列腺組織中的P物質(zhì)表達明顯高于對照組，這表明神經(jīng)內(nèi)分泌細胞與慢性前列腺炎之間存在著密切的關(guān)系，其中的作用機制可能為神經(jīng)內(nèi)分泌細胞通過其活性分泌產(chǎn)物P物質(zhì)（SP）、嗜鉻蛋白A（CgA）、神經(jīng)元特異性烯醇化酶（NSE）及神經(jīng)生長因子（NGF）等作用于前列腺上皮細胞及平滑肌細胞并影響其功能，從而引起一系列慢性前列腺炎樣癥狀。因此神經(jīng)內(nèi)分泌細胞對前列腺的生長、分化和分泌功能等起著一定的調(diào)節(jié)作用，它可能參與慢性前列腺炎的病因和發(fā)病過程。
[Abstract]:Objective to investigate the relationship between neuroendocrine cells and chronic prostatitis by studying substance P from neuroendocrine cells. Methods Forty healthy SPF male Sprague-Dawley SD rats aged 2 months were selected as experimental subjects, weighing between 220 and 275 g, and then randomly divided into two groups: model group and control group, 20 rats in each group, and 20 rats in each group were randomly divided into two groups: model group and control group, each group was divided into two groups: model group (n = 20), control group (n = 20) and control group (n = 20). All of them were fed in aseptic environment. SD rats in the model group were anesthetized by 10% chloral hydrate, castrated by abdominal castration under aseptic conditions. Penicillin was injected intramuscularly on the day of operation and 2 days after operation. No infection was observed after one week, and the wound healed well. 17 尾 -estradiol 0.25 mg / kg was injected subcutaneously into the back for 30 days, while the control group did not undergo ovariectomized, but at the same time, 0.9% saline was injected subcutaneously on the back for one month. Within 24 hours of the last injection of 17 尾 -estradiol and normal saline, the whole blood of 5ml was collected by cardiac puncture in time under aseptic condition, and then the prostate tissue was quickly removed and placed in D-Hanks fluid. Then carefully remove the prostate capsule, surrounding fat, blood vessels and other tissues in order to obtain more pure prostate tissue, and finally fixed the prostate tissue with 4% paraformaldehyde, and put it in the refrigerator at -80 鈩

本文編號：1811843